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Species: Homo sapiens
Genetic Insert: nNOS1 - CTEV
Vector Backbone Description: Vector Backbone:pTag4C; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39405910
Proper citation: RRID:Addgene_223294 Copy
Species: Synthetic
Genetic Insert: luxOFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5372; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40417169
Proper citation: RRID:Addgene_229950 Copy
Genetic Insert: Malibu-mScarlet
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39386450
Comments: Please visit ttps://doi.org/10.1101/2024.09.27.615526 for bioRxiv preprint.
Proper citation: RRID:Addgene_229554 Copy
Species: Synthetic
Genetic Insert: luxKate
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3955; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40417169
Proper citation: RRID:Addgene_229946 Copy
Species: Synthetic
Genetic Insert: luxKOk
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5372; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40417169
Proper citation: RRID:Addgene_229949 Copy
Species: Homo sapiens
Genetic Insert: GRIN2A - CTEV
Vector Backbone Description: Vector Backbone:pTag4C; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39405910
Proper citation: RRID:Addgene_223290 Copy
Species: Homo sapiens
Genetic Insert: GTSE1
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38979260
Comments: Please visit https://doi.org/10.1101/2024.06.26.600797 for bioRxiv preprint.
Proper citation: RRID:Addgene_224725 Copy
Vector Backbone Description: Backbone Size:5387; Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: "Empty" vector for rapid cloning via DNA Assembly (i.e. Gibson, HiFi, etc.) via SwaI linearization.
Proper citation: RRID:Addgene_228485 Copy
Species: E. coli
Genetic Insert: IraD
Vector Backbone Description: Backbone Size:5368; Vector Backbone:pSKB2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30975721
Proper citation: RRID:Addgene_229455 Copy
Species: E. coli
Genetic Insert: IraM
Vector Backbone Description: Backbone Size:5368; Vector Backbone:pSKB2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30975721
Proper citation: RRID:Addgene_229454 Copy
Species: E. coli
Genetic Insert: IraD (R67A)
Vector Backbone Description: Backbone Size:5368; Vector Backbone:pSKB2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30975721
Proper citation: RRID:Addgene_229457 Copy
Species: E. coli
Genetic Insert: IraD (aa 17-130 only)
Vector Backbone Description: Backbone Size:5368; Vector Backbone:pSKB2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30975721
Proper citation: RRID:Addgene_229456 Copy
Species: Homo sapiens
Genetic Insert: GTSE1
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38979260
Comments: Please visit https://doi.org/10.1101/2024.06.26.600797 for bioRxiv preprint.
Proper citation: RRID:Addgene_224727 Copy
Species: Homo sapiens
Genetic Insert: GTSE1
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38979260
Comments: Please visit https://doi.org/10.1101/2024.06.26.600797 for bioRxiv preprint.
Proper citation: RRID:Addgene_224728 Copy
Species: Synthetic
Genetic Insert: pol
Vector Backbone Description: Backbone Size:5630; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Bacterial expression of 10xHis-SUMO tagged Pfu polymerase fused to Sso7d processivity domain (commercially sold as Phusion Polymerase). Polymerase domain contains the A408S amino acid substitution for enhanced fidelity.
Kennedy EM, Hergott C, Dewhurst S, Kim B. The mechanistic architecture of thermostable Pyrococcus furiosus family B DNA polymerase motif A and its interaction with the dNTP substrate. Biochemistry. 2009 Dec 1;48(47):11161-8. doi: 10.1021/bi9010122. PMID: 19817489; PMCID: PMC3097049.
Proper citation: RRID:Addgene_228494 Copy
Species: E. coli
Genetic Insert: IraD (W64R)
Vector Backbone Description: Backbone Size:3787; Vector Backbone:pOKD5; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30975721
Proper citation: RRID:Addgene_229461 Copy
Vector Backbone Description: Backbone Size:4899; Vector Backbone:pLib; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Derived from biGBac System. Uses SmaI restriction enzyme cleavage for linearization for rapid cloning via DNA Assembly methods (e.g. Gibson, HiFi, In-Fusion, etc.)
Original citation:
Weissmann F, Petzold G, VanderLinden R, Huis In 't Veld PJ, Brown NG, Lampert F, Westermann S, Stark H, Schulman BA, Peters JM. biGBac enables rapid gene assembly for the expression of large multisubunit protein complexes. Proc Natl Acad Sci U S A. 2016 May 10;113(19):E2564-9. doi: 10.1073/pnas.1604935113. Epub 2016 Apr 25. PMID: 27114506; PMCID: PMC4868461.
Proper citation: RRID:Addgene_228479 Copy
Species: Synthetic
Genetic Insert: IL2SP-Aro6-LaM8-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39633047
Proper citation: RRID:Addgene_229999 Copy
Species: Gaussia princeps
Genetic Insert: Gaussia luciferase
Vector Backbone Description: Backbone Marker:Stephen Elledge; Vector Backbone:pINDUCER10-PheS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24889111
Comments: Please note: This plasmid contains a T nucleotide deletion in the LoxP just upstream of the IRES. This deletion is not expected to impact plasmid function.
Proper citation: RRID:Addgene_228704 Copy
Species: E. coli
Genetic Insert: RssB (E229C)
Vector Backbone Description: Backbone Size:3787; Vector Backbone:pOKD5; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30975721
Proper citation: RRID:Addgene_229477 Copy
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