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Species: Rabies
Genetic Insert: Rabies virus CVS N2c glycoprotein
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pCAG vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_244251 Copy
Species: Arabidopsis thaliana
Genetic Insert: Arabidopsis thaliana RPS5A promoter
Vector Backbone Description: Backbone Size:8151; Vector Backbone:pK7WG2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:42290098
Comments: Please visit https://doi.org/10.1101/2025.11.01.685978 for bioRxiv preprint.
Proper citation: RRID:Addgene_248055 Copy
Species: Synthetic
Genetic Insert: 5'UTR-Pa01-bpNLS-3'UTR-polyA
Vector Backbone Description: Backbone Marker:Addgene #207455; Vector Backbone:IVT-nCas9(H840A); Vector Types:Mammalian Expression, CRISPR, in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41813887
Proper citation: RRID:Addgene_248218 Copy
Species: Bacteriophage T7
Genetic Insert: T7 RNA polymerase
Vector Backbone Description: Backbone Marker:Backbone is plex_307-ccdB-blasticidin (Addgene plasmid #201136, Alejandro Chavez Lab).; Vector Backbone:pLEX307-blast; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_255667 Copy
Species: Homo sapiens
Genetic Insert: PSMF1
Vector Backbone Description: Backbone Marker:James Bradner and Behnam Nabet, addgene #91797; Vector Backbone:pLEX_305-N-dTAG; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_251199 Copy
Species: Homo sapiens
Genetic Insert: PSMF1
Vector Backbone Description: Backbone Marker:James Bradner and Behnam Nabet, addgene #91797; Vector Backbone:pLEX_305-N-dTAG; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_251197 Copy
Species: Homo sapiens
Genetic Insert: PSMF1
Vector Backbone Description: Backbone Marker:James Bradner and Behnam Nabet, addgene #91797; Vector Backbone:pLEX_305-N-dTAG; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_251196 Copy
Species: Homo sapiens
Genetic Insert: PSMF1
Vector Backbone Description: Backbone Marker:James Bradner and Behnam Nabet, addgene #91797; Vector Backbone:pLEX_305-N-dTAG; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_251195 Copy
Species: Homo sapiens
Genetic Insert: PSMF1
Vector Backbone Description: Backbone Marker:Stephen Elledge Lab; Vector Backbone:pHAGE- pEF1alpha, pGK BlastR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_251192 Copy
Species: Other
Genetic Insert: sfGFP Dropout Cassette
Vector Backbone Description: Vector Backbone:pS797; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: To enable the conjugal transformation of Geobacillus species. plasmid pS797 was engineered to include an origin of transfer (ORI T) that incorporates the Nic region and traJ gene from the RP4 conjugal plasmid. Additionally, it features two replication origins-ColE for E. coli and BST1 for Geobacillus-to support propagation in both hosts. The plasmid also carries two antibiotic resistance markers, which allow for selection with ampicillin in E. coli and kanamycin in Geobacillus.
Proper citation: RRID:Addgene_235443 Copy
Species: Synthetic
Genetic Insert: PE8a prime editor
Vector Backbone Description: Vector Backbone:pT7; Vector Types:Template for in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42168701
Proper citation: RRID:Addgene_257698 Copy
Species: Other
Genetic Insert: lacZ Dropout Cassette
Vector Backbone Description: Vector Backbone:pS797; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: To enable the conjugal transformation of Geobacillus species. plasmid pS797 was engineered to include an origin of transfer (ORI T) that incorporates the Nic region and traJ gene from the RP4 conjugal plasmid. Additionally, it features two replication origins-ColE for E. coli and BST1 for Geobacillus-to support propagation in both hosts. The plasmid also carries two antibiotic resistance markers, which allow for selection with ampicillin in E. coli and kanamycin in Geobacillus.
Proper citation: RRID:Addgene_235445 Copy
Genetic Insert: M. alvus pyrrolysyl synthetase-ASV
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38947215
Proper citation: RRID:Addgene_253745 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Vtc2p_SPX_1-182
Vector Backbone Description: Backbone Size:5206; Vector Backbone:pMH (pET24d-based); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40879985
Proper citation: RRID:Addgene_237893 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Vtc2p_SPX_1-182
Vector Backbone Description: Backbone Size:5206; Vector Backbone:pMH (pET24d-based); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40879985
Proper citation: RRID:Addgene_237894 Copy
Species: Synthetic
Genetic Insert: PE8d prime editor
Vector Backbone Description: Vector Backbone:pT7; Vector Types:Template for in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42168701
Proper citation: RRID:Addgene_257700 Copy
Species: Synthetic
Genetic Insert: FRT-mScarlet-attP(GA)-PuroR-F3
Vector Backbone Description: Vector Backbone:pBR322/pUC; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42263534
Proper citation: RRID:Addgene_231943 Copy
Vector Backbone Description: Backbone Size:4017; Vector Backbone:pTRK829; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin
Defining Citation: PMID:41556308
Proper citation: RRID:Addgene_252705 Copy
Vector Backbone Description: Backbone Size:4047; Vector Backbone:pSpy3C; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38911550
Comments: The multiple cloning site (EcoRI, XbaI, SpeI and PstI) contains the Plac_mrfp cassette. The mrfp reporter allows for red-white screening during sub-cloning in E. coli (DH5a). When the Plac_mrfp cassette is replaced with the insert of interest, colonies will appear white.
Resource Information: To create pSpy3C, we took the minimal pWV01* replicon from pBAV1K-T5-gfp (Plasmid #26702), the chloramphenicol resistance cassette from pIB166 (Plasmid #90189) and the MCS featuring the Plac_mrfp cassette from pBS3Clux (#55172).
Please download the file linked below to obtain a well annotated map of the plasmid.
Proper citation: RRID:Addgene_220253 Copy
Genetic Insert: sgRNA - cas9
Vector Backbone Description: Backbone Size:6603; Vector Backbone:pNORg10; Vector Types:E. coli - R. gnavus shuttle vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41820342
Comments: Please visit https://doi.org/10.1101/2025.08.12.669022 for bioRxiv preprint.
Proper citation: RRID:Addgene_249345 Copy
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