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Species: Homo sapiens
Genetic Insert: TARDBP
Vector Backbone Description: Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40149017
Proper citation: RRID:Addgene_235499 Copy
Species: Escherichia coli
Genetic Insert: NOT gate
Vector Backbone Description: Vector Backbone:pColA; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40234414
Comments: JM101 (working strain for phagemid transfer). Requires the dCas9 plasmid, pJ1996v2 (Addgene plasmid #140664). Please visit https://doi.org/10.1101/2024.08.28.610043 for bioRxiv preprint.
Proper citation: RRID:Addgene_235485 Copy
Species: Homo sapiens
Genetic Insert: SMAD3 LSD-CSA
Vector Backbone Description: Backbone Size:3155; Vector Backbone:pDonr-253; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_234310 Copy
Species: Homo sapiens
Genetic Insert: NOLC1 sgRNA
Vector Backbone Description: Vector Backbone:pU6-pegRNA-GG-acceptor (Addgene #132777); Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40205048
Proper citation: RRID:Addgene_234833 Copy
Species: Homo sapiens
Genetic Insert: tev-Hs TGFBR2 iso1 E526Q
Vector Backbone Description: Backbone Size:3155; Vector Backbone:pDonr-253; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Comments: No signaling through either Smad pathway
Proper citation: RRID:Addgene_234311 Copy
Species: Mus musculus
Genetic Insert: Acvr1b
Vector Backbone Description: Backbone Size:3155; Vector Backbone:pDonr-253; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_234279 Copy
Species: Homo sapiens
Genetic Insert: tev-Hs TGFBR2 iso1 R537P
Vector Backbone Description: Backbone Size:3155; Vector Backbone:pDonr-253; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Comments: Constitutive activation of Smad1/5 and loss of Smad2/3 signaling
Proper citation: RRID:Addgene_234312 Copy
Species: Homo sapiens
Genetic Insert: TARDBP
Vector Backbone Description: Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40149017
Proper citation: RRID:Addgene_235488 Copy
Species: Homo sapiens
Genetic Insert: TGFB3
Vector Backbone Description: Backbone Size:3155; Vector Backbone:pDonr-253; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Comments: Generated synthetically as no full length clones were available
Proper citation: RRID:Addgene_234298 Copy
Species: Homo sapiens
Genetic Insert: TGFB3 C227S/C229S
Vector Backbone Description: Backbone Size:3155; Vector Backbone:pDonr-253; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_234299 Copy
Species: Homo sapiens
Genetic Insert: SMAD1 S462A/S463A/S465A
Vector Backbone Description: Backbone Size:3155; Vector Backbone:pDonr-253; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_234294 Copy
Species: Homo sapiens
Genetic Insert: NPY-pHLeon
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39504473
Proper citation: RRID:Addgene_235176 Copy
Species: Escherichia coli
Genetic Insert: NOT gate
Vector Backbone Description: Vector Backbone:pColA; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40234414
Comments: JM101 (working strain for phagemid transfer). Requires the dCas9 plasmid, pJ1996v2 (Addgene plasmid #140664). Please visit https://doi.org/10.1101/2024.08.28.610043 for bioRxiv preprint.
Proper citation: RRID:Addgene_235451 Copy
Species: Escherichia coli
Genetic Insert: NOT gate
Vector Backbone Description: Vector Backbone:pColA; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40234414
Comments: JM101 (working strain for phagemid transfer). Requires the dCas9 plasmid, pJ1996v2 (Addgene plasmid #140664). Please visit https://doi.org/10.1101/2024.08.28.610043 for bioRxiv preprint.
Proper citation: RRID:Addgene_235454 Copy
Species: Mus musculus
Genetic Insert: Ezh2
Vector Backbone Description: Vector Backbone:pPB-TRE3G; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38724747
Comments: This is a piggyBAC plasmid that integrates into the genome and can be selected for via Neomycin resistance. Expression of epigenetic effector is induced via adding doxycyline to media. Succesful activiation of the epigenetic editing system is indicated by sfGFP expression.
Proper citation: RRID:Addgene_235575 Copy
Species: Mus musculus
Genetic Insert: Ring1b
Vector Backbone Description: Vector Backbone:pPB-TRE3G; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38724747
Comments: This is a piggyBAC plasmid that integrates into the genome and can be selected for via Neomycin resistance. Expression of epigenetic effector is induced via adding doxycyline to media. Succesful activiation of the epigenetic editing system is indicated by sfGFP expression.
Proper citation: RRID:Addgene_235574 Copy
Species: Escherichia coli
Genetic Insert: NOT gate
Vector Backbone Description: Vector Backbone:pColA; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40234414
Comments: JM101 (working strain for phagemid transfer). Requires the dCas9 plasmid, pJ1996v2 (Addgene plasmid #140664). Please visit https://doi.org/10.1101/2024.08.28.610043 for bioRxiv preprint.
Proper citation: RRID:Addgene_235450 Copy
Species: Synthetic
Genetic Insert: sgRNA backbone
Vector Backbone Description: Vector Backbone:pPB-U6; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38724747
Comments: Drives expression of a sub-cloned single guide RNA (gRNA) with enhanced scaffold to target epigenetic editing to a desired locus. gRNA are cloned using the BlpI and BstXI sites. This is a piggyBAC plasmid that integrates into the genome and can be selected for via Puromycin resistance. An additional tagBFP insert is expressed constituitively and indirectly indicates presence of the gRNA.
Proper citation: RRID:Addgene_235571 Copy
Species: Aeromonas phage Aes123
Genetic Insert: N-terminal Fragment of the CLm Split Intein
Vector Backbone Description: Backbone Size:5445; Vector Backbone:pMAL-c2X; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40108172
Comments: Please visit https://doi.org/10.1101/2025.01.22.634254 for bioRxiv preprint.
Proper citation: RRID:Addgene_234487 Copy
Species: Mus musculus
Genetic Insert: Prdm9
Vector Backbone Description: Vector Backbone:pPB-TRE3G; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38724747
Comments: This is a piggyBAC plasmid that integrates into the genome and can be selected for via Neomycin resistance. Expression of epigenetic effector is induced via adding doxycyline to media. Succesful activiation of the epigenetic editing system is indicated by sfGFP expression.
Proper citation: RRID:Addgene_235577 Copy
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