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Species: Homo sapiens
Genetic Insert: Trinucleotide repeat containing 6C
Vector Backbone Description: Backbone Size:5421; Vector Backbone:pFRT/TO/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18978028
Proper citation: RRID:Addgene_19885 Copy
Species: Homo sapiens
Genetic Insert: trinucleotide repeat containing 6B
Vector Backbone Description: Backbone Size:5421; Vector Backbone:pFRT/TO/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18978028
Proper citation: RRID:Addgene_19884 Copy
Species: Homo sapiens
Genetic Insert: Eukaryotic translation initiation factor 2C, 1
Vector Backbone Description: Backbone Size:5421; Vector Backbone:pFRT/TO/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18978028
Proper citation: RRID:Addgene_19887 Copy
Species: Homo sapiens
Genetic Insert: Eukaryotic translation initiation factor 2C, 3
Vector Backbone Description: Backbone Size:5421; Vector Backbone:pFRT/TO/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18978028
Proper citation: RRID:Addgene_19889 Copy
Species: Homo sapiens
Genetic Insert: Eukaryotic translation initiation factor 2C, 2
Vector Backbone Description: Backbone Size:5421; Vector Backbone:pFRT/TO/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18978028
Proper citation: RRID:Addgene_19888 Copy
Species: Homo sapiens
Genetic Insert: Cyclin D1
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9465025
Proper citation: RRID:Addgene_19927 Copy
Species: Homo sapiens
Genetic Insert: PCTAIRE-1
Vector Backbone Description: Backbone Size:6550; Vector Backbone:pCMV-neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8266103
Comments: See scanned map.
Proper citation: RRID:Addgene_1999 Copy
Species: Mus musculus
Genetic Insert: M33
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14967148
Comments: Kozak modified mouse M33 cDNA, N-terminal flag. (kingston #1390)
Proper citation: RRID:Addgene_1992 Copy
Species: Mus musculus
Genetic Insert: Ring1A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14967148
Comments: Kozak modified mouse RING1A cDNA, N-terminal flag. (kingston #1393)
Proper citation: RRID:Addgene_1995 Copy
Species: Homo sapiens
Genetic Insert: SYN-EGFP-WPRE
Vector Backbone Description: Backbone Size:7383; Vector Backbone:pTYF; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18485188
Proper citation: RRID:Addgene_19973 Copy
Species: Homo sapiens
Genetic Insert: PCTAIRE-1
Vector Backbone Description: Backbone Size:6550; Vector Backbone:pCMV-neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8266103
Comments: See scanned map.
Proper citation: RRID:Addgene_1996 Copy
Species: Homo sapiens
Genetic Insert: Rpl26
Vector Backbone Description: Backbone Size:5270; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18951086
Comments: Made by Yaara Ofir-Rosenfeld
Proper citation: RRID:Addgene_19972 Copy
Species: Homo sapiens
Genetic Insert: PCTAIRE-1
Vector Backbone Description: Backbone Size:6550; Vector Backbone:pCMV-neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8266103
Comments: See scanned map.
Proper citation: RRID:Addgene_1998 Copy
Species: Homo sapiens
Genetic Insert: GfaABC1D-tTA
Vector Backbone Description: Backbone Size:7408; Vector Backbone:pTYF; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18324737
Proper citation: RRID:Addgene_19977 Copy
Species: Homo sapiens
Genetic Insert: Fyn
Vector Backbone Description: Backbone Size:4657; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8206991
Proper citation: RRID:Addgene_19979 Copy
Species: tobacco etch virus
Genetic Insert: TEV protease
Vector Backbone Description: Backbone Marker:Herman Bujard; Backbone Size:4300; Vector Backbone:pZS*24-MCS1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10873547
Comments: This TEV protease expression vector is intended to be used for controlled intracellular processing of fusion proteins (TEV protease substrates) in E. coli. It is supplied in BL21-Pro cells so that transcription of TEV protease can be regulated by anhydrotetracycline.
Replicon: pSC101
Promoter: Synthetic ªPL/tetO
Inducer: anhydrotetracycline (100 ºg/liter)
Notes: Expression of the TEV protease gene on pKM586 will be constitutive unless the host strain produces Tet repressor. Regulated expression can be achieved in DH5alphaPro or BL21Pro cells (Clontech). For best results, perform intracellular processing experiments at 30 °C.
Proper citation: RRID:Addgene_19978 Copy
Species: E.coli
Genetic Insert: SYN-tTA
Vector Backbone Description: Backbone Size:7431; Vector Backbone:pTYF; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18324737
Proper citation: RRID:Addgene_19980 Copy
Species: Mus musculus
Genetic Insert: 2xGfaABC1D-tTA
Vector Backbone Description: Backbone Size:7425; Vector Backbone:pTYF; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18324737
Proper citation: RRID:Addgene_19981 Copy
Species: Discosoma sp
Genetic Insert: Tretight-DsRed2
Vector Backbone Description: Backbone Size:7462; Vector Backbone:pTYF; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18324737
Proper citation: RRID:Addgene_19983 Copy
Species: Homo sapiens
Genetic Insert: SGK1 3'UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6249; Vector Backbone:psiCHECK-2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18978028
Proper citation: RRID:Addgene_19865 Copy
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