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Species: Mus musculus
Genetic Insert: 14-3-3 zeta
Vector Backbone Description: Backbone Size:6100; Vector Backbone:pEBG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_1942 Copy
Species: Mus musculus
Genetic Insert: DNAJB10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Part of the HSP40 gene family
Disclaimer:
For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene.
Proper citation: RRID:Addgene_19560 Copy
Species: Mus musculus
Genetic Insert: DNAJB10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Part of the HSP40 gene family
Disclaimer:
For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene.
Proper citation: RRID:Addgene_19533 Copy
Species: Mus musculus
Genetic Insert: oligo targeting p53
Vector Backbone Description: Backbone Size:7926; Vector Backbone:MSCV FLIP; Vector Types:Mammalian Expression, Retroviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
References:
Comments: Constitutively expresses puromycin-resistance and the surface molecule Thy1.1. Recombines to express GFP and miR30-based RNAi.
There are minor discrepancies between the depositor's sequence and Addgene's sequencing results. The mismatches are either coding joints or a sequencing difference between the actual and the published miR30 vector sequence.
See FLIP vector protocols (PDF from this page) for more information.
Proper citation: RRID:Addgene_19745 Copy
Species: Mus musculus
Genetic Insert: Fly engrailed 36240
Vector Backbone Description: Backbone Size:4798; Vector Backbone:CAGEN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19714 Copy
Species: Mus musculus
Genetic Insert: Merlin I
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pXJ40-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19700 Copy
Species: Mus musculus
Genetic Insert: Nore1
Vector Backbone Description: Backbone Size:4657; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_1979 Copy
Species: Mus musculus
Genetic Insert: Oct4
Vector Backbone Description: Backbone Size:32900; Vector Backbone:pAd-ClaI; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
References:
Comments: From article: For the cloning of adenoviral vectors, EcoRI fragments containing the respective cDNAs
for c-myc (constitutively active human T58A variant), Klf4, Oct4 and Sox2 were
integrated into the EcoRI site of pHIHG-Ad2. A fragment containing the cDNA sequence
was isolated from the resulting plasmids by MfeI/PacI digestion and used for
electroporation of BJ5183 cells together with linearized vector pAd-ClaI. Correct clones
were identified by HindIII digestion.
Proper citation: RRID:Addgene_19768 Copy
Species: Mus musculus
Genetic Insert: Sox2
Vector Backbone Description: Backbone Size:32900; Vector Backbone:pAd-ClaI; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
References:
Comments: From article: For the cloning of adenoviral vectors, EcoRI fragments containing the respective cDNAs
for c-myc (constitutively active human T58A variant), Klf4, Oct4 and Sox2 were
integrated into the EcoRI site of pHIHG-Ad2. A fragment containing the cDNA sequence
was isolated from the resulting plasmids by MfeI/PacI digestion and used for
electroporation of BJ5183 cells together with linearized vector pAd-ClaI. Correct clones
were identified by HindIII digestion.
Proper citation: RRID:Addgene_19767 Copy
Species: Mus musculus
Genetic Insert: RASSF1C
Vector Backbone Description: Backbone Size:4657; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_1981 Copy
Species: Mus musculus
Genetic Insert: mPh1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: C-terminal flag-tagged full-length mouse PH1 cDNA for protein expression (kingston #1388)
Proper citation: RRID:Addgene_1985 Copy
Species: Mus musculus
Genetic Insert: M33
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: Kozak modified mouse M33 cDNA, untagged. (kingston #1389)
Proper citation: RRID:Addgene_1986 Copy
Species: Mus musculus
Genetic Insert: Telomere-Repeat binding Factor 1
Vector Backbone Description: Backbone Marker:Scott Lowe; Backbone Size:5600; Vector Backbone:pWzl-hygro; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19834 Copy
Species: Mus musculus
Genetic Insert: Oct3/4-2A-Klf4-2A-Sox2
Vector Backbone Description: Backbone Marker:Jun-ichi Miyazaki; Backbone Size:4778; Vector Backbone:pCX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Addgene has verified the insert size with an EcoRI digest. To see a picture of the digest, click on "Reviews" under Plasmid Links, to the right of this page.
Proper citation: RRID:Addgene_19771 Copy
Species: Mus musculus
Genetic Insert: Klf4
Vector Backbone Description: Backbone Size:32900; Vector Backbone:pAd-ClaI; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
References:
Comments: From article: For the cloning of adenoviral vectors, EcoRI fragments containing the respective cDNAs
for c-myc (constitutively active human T58A variant), Klf4, Oct4 and Sox2 were
integrated into the EcoRI site of pHIHG-Ad2. A fragment containing the cDNA sequence
was isolated from the resulting plasmids by MfeI/PacI digestion and used for
electroporation of BJ5183 cells together with linearized vector pAd-ClaI. Correct clones
were identified by HindIII digestion.
Proper citation: RRID:Addgene_19770 Copy
Species: Mus musculus
Genetic Insert: c-Myc
Vector Backbone Description: Backbone Marker:Jun-ichi Miyazaki; Backbone Size:4778; Vector Backbone:pCX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19772 Copy
Species: Mus musculus
Genetic Insert: M33
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: Kozak modified mouse M33 cDNA, C-terminal flag. (kingston #1391)
Proper citation: RRID:Addgene_1993 Copy
Species: Mus musculus
Genetic Insert: Ring1A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: Kozak modified mouse RING1A cDNA, untagged. (kingston #1392)
Proper citation: RRID:Addgene_1994 Copy
Species: Mus musculus
Genetic Insert: AID 3' UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2000; Vector Backbone:pGEM-luc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19988 Copy
Species: Mus musculus
Genetic Insert: AID 3' UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2000; Vector Backbone:pGEM-luc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19987 Copy
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