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  • Organism:mus musculus (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pLHCX-Flag-mPKM2
 
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RRID:Addgene_42512 Pyruvate Kinase M2 Mus musculus Ampicillin PMID:22052977 Plasmid constructed by Heather Christofk in Lew Cantley's Lab. Ref: Christofk, H. R., Vander Heiden, M. G., Harris, M. H., Ramanathan, A., Gerszten, R. E., Wei, R., Fleming, M. D., et al. (2008). The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth. Nature, 452(7184), 230–233. doi:10.1038/nature06734 Backbone Marker:Clontech; Backbone Size:6800; Vector Backbone:pLHCX; Vector Types:Retroviral; Bacterial Resistance:Ampicillin 2026-08-15 01:14:54 0
pLHCX-Flag-mPKM1
 
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1+ mentions
RRID:Addgene_42511 Pyruvate Kinase M1 Mus musculus Ampicillin PMID:22052977 Plasmid constructed by Heather Christofk in Lew Cantley's Lab. Ref: Christofk, H. R., Vander Heiden, M. G., Harris, M. H., Ramanathan, A., Gerszten, R. E., Wei, R., Fleming, M. D., et al. (2008). The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth. Nature, 452(7184), 230–233. doi:10.1038/nature06734 The depositing scientist notes: NM_011099.3 is PKM2 and NM_001253883.1 is PKM1, based on sequence homology of the respective translated products to the human PKM isoforms. The isoform annotations in the corresponding GeneBank entries are incorrect. Backbone Marker:Clontech; Backbone Size:6800; Vector Backbone:pLHCX; Vector Types:Retroviral; Bacterial Resistance:Ampicillin 2026-08-15 01:14:54 2
pcDNA 3.1/V5-His TOPO TA mouse Mns1
 
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RRID:Addgene_42899 Mns1 Mus musculus Ampicillin PMID:22396656 The cDNA encoding full-length mouse Mns1, complete CDS with Kozak sequence, was cloned into pcDNA 3.1/V5-His TOPO TA (Invitrogen) for V5 tagging. Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA 3.1/V5-His TOPO TA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:58 0
GST-ENLYFQC-PNT(46-138)-His6
 
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RRID:Addgene_42919 Ets-1 PNT domain (46-138) Mus musculus Ampicillin PMID:19681083 The gene fragment encoding residues 46-138 of Ets-1 (including the PNT domain) was amplified from an Ets-1 plasmid using primers to insert 5’ EcoR1 and 3’ Not1 restriction sites for incorporation into a pGEX-4T-2 vector. The primers also encoded an amino-terminal TEV protease cleavage site (ENLYFQ//C) and a carboxy-terminal hexahistidine tag. For this amplification the following PCR primers were used: 5’- GCC GGA ATT CGT GAA AAC CTG TAT TTT CAG TGC TCC CAA GCC TTG AAA GCT -3’ 5’- GCC CCC TTT TGC GGC CGC CTA GTG ATG GTG ATG GTG ATG ACC TTT CAC ATC CTC TTT CTG -3’ The PCR-amplified fragments were digested with Not1 and EcoR1 and ligated to Not1/EcoR1- digested and CIP-treated pGEX-4T-2. The protein coding sequence of the Ets-1 PNT domain is as follows: SQALK ATFSGFTKEQ QRLGIPKDPR QWTETHVRDW VMWAVNEFSL KGVDFQKFCM NGAALCALGK DCFLELAPDF VGDILWEHLE ILQKEDVK Alternate plasmid names: GST-TEV-PNT(46-138)-His6, GST-PNT, pGEX-PNT, GST-PNT-pGEX, GST-ENLYFQC-Ets-1(46-138)-His6, GST-TEV-Ets-1(46-138)-His6, GST-Ets-1(46-138), pGEX-Ets-1(46-138), GST-Ets-1(46-138)-pGEX Backbone Marker:Amersham; Backbone Size:4970; Vector Backbone:pGEX-4T-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Contains the PNT domain (Serine 46 through Lysine 138) 2026-08-15 01:14:58 0
MLM3636-Prnp-CDS-Neg
 
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RRID:Addgene_61856 PrP guiding sequence Mus musculus Ampicillin PMID:25490046 Backbone Marker:Joung lab MLM3636 ; Backbone Size:2278; Vector Backbone:MLM3636; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:17:47 0
MLM3636-Prnp-CDS-Pos
 
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RRID:Addgene_61857 PrP guiding sequence Mus musculus Ampicillin PMID:25490046 Backbone Marker:Joung lab MLM3636 ; Backbone Size:2278; Vector Backbone:MLM3636; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:17:46 0
pInducer21 Flag-Sesn3
 
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RRID:Addgene_61866 Sesn3 Mus musculus Ampicillin PMID:25259925 Backbone Size:13000; Vector Backbone:pInducer21; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin none 2026-08-15 01:17:47 0
pLJM1 Flag-Sesn2 419A/422A/426A
 
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RRID:Addgene_61872 Sesn2 Mus musculus Ampicillin PMID:25259925 Backbone Size:7000; Vector Backbone:pLJM1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 419A/422A/426A 2026-08-15 01:17:47 0
pGEX-6p-1 Sesn2
 
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RRID:Addgene_61873 Sesn2 Mus musculus Ampicillin PMID:25259925 Backbone Size:6000; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin none 2026-08-15 01:17:47 0
pGEX-6p-1 Sesn3
 
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RRID:Addgene_61875 Sesn3 Mus musculus Ampicillin PMID:25259925 Backbone Size:6000; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin none 2026-08-15 01:17:47 0
pLJM1 Flag-Sesn2
 
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RRID:Addgene_61871 Sesn2 Mus musculus Ampicillin PMID:25259925 Backbone Size:7000; Vector Backbone:pLJM1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin none 2026-08-15 01:17:47 0
pcDNA3.1(+) neo with Smad5 CDS
 
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RRID:Addgene_61800 Smad5 CDS Mus musculus Ampicillin PMID:25798844 Backbone Size:6000; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:17:46 0
pInducer21 Flag-Sesn2
 
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RRID:Addgene_61829 Sesn2 Mus musculus Ampicillin PMID:25259925 Backbone Size:13000; Vector Backbone:pInducer21; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin none 2026-08-15 01:17:47 0
KHLH31 pEGFP-N2 (1089)
 
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RRID:Addgene_62032 KHLH31 Mus musculus Kanamycin PMID:20876400 Backbone Marker:Clontech; Vector Backbone:pEGFP-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:17:48 0
NET29A.Mm pEGFP-N2 (1326)
 
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RRID:Addgene_62045 NET29A Mus musculus Kanamycin assembled from 2 pieces Backbone Marker:Clontech; Vector Backbone:pEGFP-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:17:49 0
NET29b.Mm pEGFP-N2 (1327)
 
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RRID:Addgene_62046 NET29b Mus musculus Kanamycin Backbone Marker:Clontech; Vector Backbone:pEGFP-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:17:48 0
Proinsulin-NanoLuc in pLX304
 
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10+ mentions
RRID:Addgene_62057 Proinsulin-NanoLuc Mus musculus Ampicillin PMID:25565210 Backbone Size:9377; Vector Backbone:pLX304; Vector Types:Mammalian Expression, Lentiviral, Luciferase; Bacterial Resistance:Ampicillin Inserted NanoLuc into C-peptide of mouse Ins2 coding sequence, with added PC2 sites flanking the luciferase 2026-08-15 01:17:49 11
pET28-p53 (Mouse 1-87)
 
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RRID:Addgene_62061 p53 Mus musculus Kanamycin PMID:22195023 Includes a thrombin cleavage site. Backbone Marker:Novagen; Backbone Size:5639; Vector Backbone:pET28A; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin contains TAD residues 1-87 2026-08-15 01:17:49 0
Teto Ngn1
 
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RRID:Addgene_62222 Neurog1 Mus musculus Ampicillin PMID:25420069 Vector Backbone:pFU Teto; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:17:51 0
Teto Ngn2
 
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RRID:Addgene_62223 Neurog2 Mus musculus Ampicillin PMID:25420069 Vector Backbone:pFU Teto; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:17:51 0

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