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Species: Mus musculus
Genetic Insert: mNgn2
Vector Backbone Description: Vector Backbone:pMXs-gw; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40086435
Proper citation: RRID:Addgene_233191 Copy
Species: Mus musculus
Genetic Insert: c-Myc
Vector Backbone Description: Vector Backbone:pMXs-gw; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40086434
Comments: Please visit https://doi.org/10.1101/2023.11.26.568736 for bioRxiv preprint.
Proper citation: RRID:Addgene_233183 Copy
Species: Mus musculus
Genetic Insert: mNgn2
Vector Backbone Description: Vector Backbone:pMXs-gw; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40086435
Proper citation: RRID:Addgene_233189 Copy
Species: Mus musculus
Genetic Insert: Grik1 promoter
Vector Backbone Description: Vector Backbone:AAV-RedO-GFP; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39903280
Comments: Cloned at Cepko Lab by Sean K. Wang based on a Grik1 enhancer previously identified by Sylvain W. Lapan (PMID: 31110282).
Please visit https://doi.org/10.1101/2023.11.02.565326 for bioRxiv preprint.
Proper citation: RRID:Addgene_233645 Copy
Species: Mus musculus
Genetic Insert: Ascl1
Vector Backbone Description: Vector Backbone:pMXs-gw; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40086434
Comments: Please visit https://doi.org/10.1101/2023.11.26.568736 for bioRxiv preprint.
Proper citation: RRID:Addgene_233152 Copy
Species: Mus musculus
Genetic Insert: NIL
Vector Backbone Description: Vector Backbone:pMXs-gw; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40086434
Comments: Please visit https://doi.org/10.1101/2023.11.26.568736 for bioRxiv preprint.
Proper citation: RRID:Addgene_233154 Copy
Species: Mus musculus
Genetic Insert: Lhx3
Vector Backbone Description: Vector Backbone:pMXs-gw; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40086434
Comments: Please visit https://doi.org/10.1101/2023.11.26.568736 for bioRxiv preprint.
Proper citation: RRID:Addgene_233150 Copy
Species: Mus musculus
Genetic Insert: Brn2
Vector Backbone Description: Vector Backbone:pMXs-gw; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40086434
Comments: Please visit https://doi.org/10.1101/2023.11.26.568736 for bioRxiv preprint.
Proper citation: RRID:Addgene_233151 Copy
Species: Mus musculus
Genetic Insert: mNgn2x3HA-P2A-mRuby2
Vector Backbone Description: Vector Backbone:pMXs-gw; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40086434
Comments: Please visit https://doi.org/10.1101/2023.11.26.568736 for bioRxiv preprint.
Proper citation: RRID:Addgene_233157 Copy
Species: Mus musculus
Genetic Insert: Isl1
Vector Backbone Description: Vector Backbone:pMXs-gw; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40086434
Comments: Please visit https://doi.org/10.1101/2023.11.26.568736 for bioRxiv preprint.
Proper citation: RRID:Addgene_233149 Copy
Species: Mus musculus
Genetic Insert: Ngn2
Vector Backbone Description: Vector Backbone:pMXs-gw; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40086434
Comments: Please visit https://doi.org/10.1101/2023.11.26.568736 for bioRxiv preprint.
Proper citation: RRID:Addgene_233148 Copy
Species: Mus musculus
Genetic Insert: mSox2 HMG
Vector Backbone Description: Vector Backbone:pHAGE2-TetO; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39543096
Proper citation: RRID:Addgene_231532 Copy
Species: Mus musculus
Genetic Insert: p53DD
Vector Backbone Description: Backbone Size:1600; Vector Backbone:pENTR4; Vector Types:in vitro transcription; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42062565
Comments: The 5' and 3' beta globin UTR sequences flanking the ORF were based on previous literature for effective modRNA expression in hiPSCs: 10.1016/j.crmeth.2022.100290
To generate linear IVT template, digest the plasmid with MluI. The vector encodes a segmented polyA track, obviating the need for polyadenylation after IVT (doi: 10.1261/rna.069286.118).
Due to the inability of nanopore sequencing to accurately read long polyN stretches and the possibility of polyA truncation, the actual length of the polyA tail may differ when propagating the vector. In our hands, we have not seen measurable differences in expression across different plasmid preparations.
Proper citation: RRID:Addgene_232751 Copy
Species: Mus musculus
Genetic Insert: mouse homeostatic iron regulator with H67D mutation
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_233040 Copy
Species: Mus musculus
Genetic Insert: ITGA5
Vector Backbone Description: Vector Backbone:pLentiCRISPR V2 Neo; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37577760
Proper citation: RRID:Addgene_233251 Copy
Species: Mus musculus
Genetic Insert: ITGA5
Vector Backbone Description: Vector Backbone:pLentiCRISPR V2 Neo; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37577760
Proper citation: RRID:Addgene_233252 Copy
Species: Mus musculus
Genetic Insert: ITGA5
Vector Backbone Description: Vector Backbone:pLentiCRISPR V2 Neo; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37577760
Proper citation: RRID:Addgene_233253 Copy
Species: Mus musculus
Genetic Insert: ITGB1
Vector Backbone Description: Vector Backbone:pLentiCRISPR V2 Neo; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37577760
Proper citation: RRID:Addgene_233250 Copy
Species: Mus musculus
Genetic Insert: ITGB1
Vector Backbone Description: Vector Backbone:pLentiCRISPR V2 Neo; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37577760
Proper citation: RRID:Addgene_233249 Copy
Species: Mus musculus
Genetic Insert: Flag-Halo-CTCF WT
Vector Backbone Description: Backbone Marker:Benoit Bruneau (Addgene #156432); Vector Backbone:pEN366; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_232930 Copy
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