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Species: Mus musculus
Genetic Insert: Pyruvate Kinase M2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6800; Vector Backbone:pLHCX; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22052977
Comments: Plasmid constructed by Heather Christofk in Lew Cantley's Lab. Ref: Christofk, H. R., Vander Heiden, M. G., Harris, M. H., Ramanathan, A., Gerszten, R. E., Wei, R., Fleming, M. D., et al. (2008). The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth. Nature, 452(7184), 230–233. doi:10.1038/nature06734
Proper citation: RRID:Addgene_42512 Copy
Species: Mus musculus
Genetic Insert: Pyruvate Kinase M1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6800; Vector Backbone:pLHCX; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22052977
Comments: Plasmid constructed by Heather Christofk in Lew Cantley's Lab. Ref: Christofk, H. R., Vander Heiden, M. G., Harris, M. H., Ramanathan, A., Gerszten, R. E., Wei, R., Fleming, M. D., et al. (2008). The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth. Nature, 452(7184), 230–233. doi:10.1038/nature06734
The depositing scientist notes: NM_011099.3 is PKM2 and NM_001253883.1 is PKM1, based on sequence homology of the respective translated products to the human PKM isoforms. The isoform annotations in the corresponding GeneBank entries are incorrect.
Proper citation: RRID:Addgene_42511 Copy
Species: Mus musculus
Genetic Insert: Mns1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA 3.1/V5-His TOPO TA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22396656
Comments: The cDNA encoding full-length mouse Mns1, complete CDS with Kozak sequence, was cloned into pcDNA 3.1/V5-His TOPO TA (Invitrogen) for V5 tagging.
Proper citation: RRID:Addgene_42899 Copy
Species: Mus musculus
Genetic Insert: Ets-1 PNT domain (46-138)
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4970; Vector Backbone:pGEX-4T-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19681083
Comments: The gene fragment encoding residues 46-138 of Ets-1 (including the PNT domain) was amplified from an Ets-1 plasmid using primers to insert 5’ EcoR1 and 3’ Not1 restriction sites for incorporation into a pGEX-4T-2 vector. The primers also encoded an amino-terminal TEV protease cleavage site (ENLYFQ//C) and a carboxy-terminal hexahistidine tag. For this amplification the following PCR primers were used:
5’- GCC GGA ATT CGT GAA AAC CTG TAT TTT CAG TGC TCC CAA GCC TTG AAA
GCT -3’
5’- GCC CCC TTT TGC GGC CGC CTA GTG ATG GTG ATG GTG ATG ACC TTT CAC
ATC CTC TTT CTG -3’
The PCR-amplified fragments were digested with Not1 and EcoR1 and ligated to Not1/EcoR1- digested and CIP-treated pGEX-4T-2.
The protein coding sequence of the Ets-1 PNT domain is as follows:
SQALK ATFSGFTKEQ QRLGIPKDPR QWTETHVRDW VMWAVNEFSL KGVDFQKFCM NGAALCALGK DCFLELAPDF VGDILWEHLE ILQKEDVK
Alternate plasmid names: GST-TEV-PNT(46-138)-His6, GST-PNT, pGEX-PNT, GST-PNT-pGEX, GST-ENLYFQC-Ets-1(46-138)-His6, GST-TEV-Ets-1(46-138)-His6, GST-Ets-1(46-138), pGEX-Ets-1(46-138), GST-Ets-1(46-138)-pGEX
Proper citation: RRID:Addgene_42919 Copy
Species: Mus musculus
Genetic Insert: PrP guiding sequence
Vector Backbone Description: Backbone Marker:Joung lab MLM3636 ; Backbone Size:2278; Vector Backbone:MLM3636; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25490046
Proper citation: RRID:Addgene_61856 Copy
Species: Mus musculus
Genetic Insert: PrP guiding sequence
Vector Backbone Description: Backbone Marker:Joung lab MLM3636 ; Backbone Size:2278; Vector Backbone:MLM3636; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25490046
Proper citation: RRID:Addgene_61857 Copy
Species: Mus musculus
Genetic Insert: Sesn3
Vector Backbone Description: Backbone Size:13000; Vector Backbone:pInducer21; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25259925
Proper citation: RRID:Addgene_61866 Copy
Species: Mus musculus
Genetic Insert: Sesn2
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pLJM1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25259925
Proper citation: RRID:Addgene_61872 Copy
Species: Mus musculus
Genetic Insert: Sesn2
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25259925
Proper citation: RRID:Addgene_61873 Copy
Species: Mus musculus
Genetic Insert: Sesn3
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25259925
Proper citation: RRID:Addgene_61875 Copy
Species: Mus musculus
Genetic Insert: Sesn2
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pLJM1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25259925
Proper citation: RRID:Addgene_61871 Copy
Species: Mus musculus
Genetic Insert: Smad5 CDS
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25798844
Proper citation: RRID:Addgene_61800 Copy
Species: Mus musculus
Genetic Insert: Sesn2
Vector Backbone Description: Backbone Size:13000; Vector Backbone:pInducer21; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25259925
Proper citation: RRID:Addgene_61829 Copy
Species: Mus musculus
Genetic Insert: KHLH31
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20876400
Proper citation: RRID:Addgene_62032 Copy
Species: Mus musculus
Genetic Insert: NET29A
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: assembled from 2 pieces
Proper citation: RRID:Addgene_62045 Copy
Species: Mus musculus
Genetic Insert: NET29b
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_62046 Copy
Species: Mus musculus
Genetic Insert: Proinsulin-NanoLuc
Vector Backbone Description: Backbone Size:9377; Vector Backbone:pLX304; Vector Types:Mammalian Expression, Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25565210
Proper citation: RRID:Addgene_62057 Copy
Species: Mus musculus
Genetic Insert: p53
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5639; Vector Backbone:pET28A; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22195023
Comments: Includes a thrombin cleavage site.
Proper citation: RRID:Addgene_62061 Copy
Species: Mus musculus
Genetic Insert: Neurog1
Vector Backbone Description: Vector Backbone:pFU Teto; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25420069
Proper citation: RRID:Addgene_62222 Copy
Species: Mus musculus
Genetic Insert: Neurog2
Vector Backbone Description: Vector Backbone:pFU Teto; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25420069
Proper citation: RRID:Addgene_62223 Copy
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