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Species: Homo sapiens
Genetic Insert: ID1
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:p15A-PB; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Kanamycin
Comments: Genomic sequence subcloned by recombineering from a clone of RPCI-11 human genomic DNA library. The exact DNA sequence in this plasmid differs somewhat from the reference human genome sequence because the reference sequence is a composite sequence, i.e., the reference ID1 region sequence may not be derived from the RPCI-11 BAC clone utilized for this construct.
The ID1 intron was placed after G198, i.e., after about a third of the sequence encoding the active site of the cre protein. See https://doi.org/10.1073/pnas.2011448117 and https://doi.org/10.1093%2Fnar%2Fgkq384. Thus, without splicing, no catalytically active cre protein sequence is expected to be translated from this sequence.
Please make sure the plasmid DNA preparation is pure by gel electrophoresis before using.
Proper citation: RRID:Addgene_139280 Copy
Species: Homo sapiens
Genetic Insert: sgRNA to insert BRCA2 V572I using base editing
Vector Backbone Description: Backbone Size:2633; Vector Backbone:B52 (Addgene #100708); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32160537
Proper citation: RRID:Addgene_139321 Copy
Species: Homo sapiens
Genetic Insert: sgRNA to insert BRCA2 S3070F using base editing
Vector Backbone Description: Backbone Size:2633; Vector Backbone:B52 (Addgene #100708); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32160537
Proper citation: RRID:Addgene_139325 Copy
Species: Homo sapiens
Genetic Insert: sgRNA to insert BRCA2 V2102I using base editing
Vector Backbone Description: Backbone Size:2633; Vector Backbone:B52 (Addgene #100708); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32160537
Proper citation: RRID:Addgene_139322 Copy
Species: Homo sapiens
Genetic Insert: sgRNA to insert BRCA2 E2772K using base editing
Vector Backbone Description: Backbone Size:2633; Vector Backbone:B52 (Addgene #100708); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32160537
Proper citation: RRID:Addgene_139323 Copy
Species: Homo sapiens
Genetic Insert: sgRNA to insert BRCA1 T922I using base editing
Vector Backbone Description: Backbone Size:2633; Vector Backbone:B52 (Addgene #100708); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32160537
Proper citation: RRID:Addgene_139328 Copy
Species: Homo sapiens
Genetic Insert: sgRNA to insert BRCA1 E638K using base editing
Vector Backbone Description: Backbone Size:2633; Vector Backbone:B52 (Addgene #100708); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32160537
Proper citation: RRID:Addgene_139327 Copy
Species: Homo sapiens
Genetic Insert: FLAG-tag NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform)
Vector Backbone Description: Backbone Marker:PMID: 24048477; Vector Backbone:RRL.sin.cPPT.CMV/IRES-puro.WPRE; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30478388
Proper citation: RRID:Addgene_139447 Copy
Species: Homo sapiens
Genetic Insert: sgRNA to insert BRCA1 Q1779Stop using base editing
Vector Backbone Description: Backbone Size:2633; Vector Backbone:B52 (Addgene #100708); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32160537
Proper citation: RRID:Addgene_139331 Copy
Species: Homo sapiens
Genetic Insert: sgRNA to insert FANCD2 Q223Stop using base editing
Vector Backbone Description: Backbone Size:2633; Vector Backbone:B52 (Addgene #100708); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32160537
Proper citation: RRID:Addgene_139332 Copy
Species: Homo sapiens
Genetic Insert: TYK2 cDNA with 267nt of 5' UTR
Vector Backbone Description: Vector Backbone:pRC/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31961910
Proper citation: RRID:Addgene_139344 Copy
Species: Homo sapiens
Genetic Insert: TYK2 cDNA with 267nt of 5' UTR
Vector Backbone Description: Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11399767
Proper citation: RRID:Addgene_139354 Copy
Species: Homo sapiens
Genetic Insert: TYK2 cDNA with 267nt of 5' UTR
Vector Backbone Description: Vector Backbone:pRC/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23359498
Proper citation: RRID:Addgene_139351 Copy
Species: Homo sapiens
Genetic Insert: JAK1 cDNA with 300nt of 5' UTR
Vector Backbone Description: Vector Backbone:pRC/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23359498
Proper citation: RRID:Addgene_139358 Copy
Species: Homo sapiens
Genetic Insert: TYK2 cDNA with aa 1-16 of human Lck fused to the N-terminus
Vector Backbone Description: Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11399767
Proper citation: RRID:Addgene_139355 Copy
Species: Homo sapiens
Genetic Insert: JAK1 cDNA with 300nt of 5'UTR
Vector Backbone Description: Vector Backbone:pRC/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9733772
Proper citation: RRID:Addgene_139356 Copy
Species: Homo sapiens
Genetic Insert: RPB1
Vector Backbone Description: Backbone Size:5135; Vector Backbone:pFRT-TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32142654
Proper citation: RRID:Addgene_139406 Copy
Species: Homo sapiens
Genetic Insert: RPB1
Vector Backbone Description: Backbone Size:5135; Vector Backbone:pFRT-TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32142654
Proper citation: RRID:Addgene_139409 Copy
Species: Homo sapiens
Genetic Insert: RPB1
Vector Backbone Description: Backbone Size:5135; Vector Backbone:pFRT-TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32142654
Proper citation: RRID:Addgene_139407 Copy
Species: Homo sapiens
Genetic Insert: GR
Vector Backbone Description: Backbone Size:2618; Vector Backbone:pDONR-P2rP3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_139538 Copy
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