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Species: Mus musculus
Genetic Insert: oligo targeting p53
Vector Backbone Description: Backbone Size:7926; Vector Backbone:MSCV FLIP; Vector Types:Mammalian Expression, Retroviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
References:
Comments: Constitutively expresses puromycin-resistance and the surface molecule Thy1.1. Recombines to express GFP and miR30-based RNAi.
There are minor discrepancies between the depositor's sequence and Addgene's sequencing results. The mismatches are either coding joints or a sequencing difference between the actual and the published miR30 vector sequence.
See FLIP vector protocols (PDF from this page) for more information.
Proper citation: RRID:Addgene_19745 Copy
Species: Mus musculus
Genetic Insert: Fly engrailed 36240
Vector Backbone Description: Backbone Size:4798; Vector Backbone:CAGEN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19714 Copy
Species: Mus musculus
Genetic Insert: Merlin I
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pXJ40-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19700 Copy
Species: Mus musculus
Genetic Insert: Nore1
Vector Backbone Description: Backbone Size:4657; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_1979 Copy
Species: Mus musculus
Genetic Insert: Oct4
Vector Backbone Description: Backbone Size:32900; Vector Backbone:pAd-ClaI; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
References:
Comments: From article: For the cloning of adenoviral vectors, EcoRI fragments containing the respective cDNAs
for c-myc (constitutively active human T58A variant), Klf4, Oct4 and Sox2 were
integrated into the EcoRI site of pHIHG-Ad2. A fragment containing the cDNA sequence
was isolated from the resulting plasmids by MfeI/PacI digestion and used for
electroporation of BJ5183 cells together with linearized vector pAd-ClaI. Correct clones
were identified by HindIII digestion.
Proper citation: RRID:Addgene_19768 Copy
Species: Mus musculus
Genetic Insert: Sox2
Vector Backbone Description: Backbone Size:32900; Vector Backbone:pAd-ClaI; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
References:
Comments: From article: For the cloning of adenoviral vectors, EcoRI fragments containing the respective cDNAs
for c-myc (constitutively active human T58A variant), Klf4, Oct4 and Sox2 were
integrated into the EcoRI site of pHIHG-Ad2. A fragment containing the cDNA sequence
was isolated from the resulting plasmids by MfeI/PacI digestion and used for
electroporation of BJ5183 cells together with linearized vector pAd-ClaI. Correct clones
were identified by HindIII digestion.
Proper citation: RRID:Addgene_19767 Copy
Species: Mus musculus
Genetic Insert: RASSF1C
Vector Backbone Description: Backbone Size:4657; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_1981 Copy
Species: Mus musculus
Genetic Insert: mPh1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: C-terminal flag-tagged full-length mouse PH1 cDNA for protein expression (kingston #1388)
Proper citation: RRID:Addgene_1985 Copy
Species: Mus musculus
Genetic Insert: M33
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: Kozak modified mouse M33 cDNA, untagged. (kingston #1389)
Proper citation: RRID:Addgene_1986 Copy
Species: Mus musculus
Genetic Insert: Telomere-Repeat binding Factor 1
Vector Backbone Description: Backbone Marker:Scott Lowe; Backbone Size:5600; Vector Backbone:pWzl-hygro; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19834 Copy
Species: Mus musculus
Genetic Insert: Oct3/4-2A-Klf4-2A-Sox2
Vector Backbone Description: Backbone Marker:Jun-ichi Miyazaki; Backbone Size:4778; Vector Backbone:pCX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Addgene has verified the insert size with an EcoRI digest. To see a picture of the digest, click on "Reviews" under Plasmid Links, to the right of this page.
Proper citation: RRID:Addgene_19771 Copy
Species: Mus musculus
Genetic Insert: Klf4
Vector Backbone Description: Backbone Size:32900; Vector Backbone:pAd-ClaI; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
References:
Comments: From article: For the cloning of adenoviral vectors, EcoRI fragments containing the respective cDNAs
for c-myc (constitutively active human T58A variant), Klf4, Oct4 and Sox2 were
integrated into the EcoRI site of pHIHG-Ad2. A fragment containing the cDNA sequence
was isolated from the resulting plasmids by MfeI/PacI digestion and used for
electroporation of BJ5183 cells together with linearized vector pAd-ClaI. Correct clones
were identified by HindIII digestion.
Proper citation: RRID:Addgene_19770 Copy
Species: Mus musculus
Genetic Insert: c-Myc
Vector Backbone Description: Backbone Marker:Jun-ichi Miyazaki; Backbone Size:4778; Vector Backbone:pCX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19772 Copy
Species: Mus musculus
Genetic Insert: M33
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: Kozak modified mouse M33 cDNA, C-terminal flag. (kingston #1391)
Proper citation: RRID:Addgene_1993 Copy
Species: Mus musculus
Genetic Insert: Ring1A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: Kozak modified mouse RING1A cDNA, untagged. (kingston #1392)
Proper citation: RRID:Addgene_1994 Copy
Species: Mus musculus
Genetic Insert: AID 3' UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2000; Vector Backbone:pGEM-luc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19988 Copy
Species: Mus musculus
Genetic Insert: AID 3' UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2000; Vector Backbone:pGEM-luc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19987 Copy
Species: Mus musculus
Genetic Insert: Il12b promoter
Vector Backbone Description: Backbone Size:5598; Vector Backbone:pGL2B; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: Luciferase Reporter driven by 400 base pair promoter of the Il12b gene.
Proper citation: RRID:Addgene_20020 Copy
Species: Mus musculus
Genetic Insert: Nfkb1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:cFlag pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: See cFlag pcDNA3 plasmid for backbone information. 5' insert restriction site flanked by kozak/start site (CCACCATG).
Proper citation: RRID:Addgene_20018 Copy
Species: Mus musculus
Genetic Insert: c-Rel
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:cFlag pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: See cFlag pcDNA3 plasmid for backbone information. 5' insert restriction site flanked by kozak/start site (CCACCATG).
Proper citation: RRID:Addgene_20016 Copy
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