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Species: Mus musculus
Genetic Insert: Daxx
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19283 Copy
Species: Mus musculus
Genetic Insert: Daxx myc S669A
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19282 Copy
Species: Mus musculus
Genetic Insert: zeta globin
Vector Backbone Description: Backbone Size:3000; Vector Backbone:Sp64; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19262 Copy
Species: Mus musculus
Genetic Insert: GAL4
Vector Backbone Description: Backbone Size:7023; Vector Backbone:MMTV-SV40-Bssk; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19255 Copy
Species: Mus musculus
Genetic Insert: mKGFR (negative dominant)
Vector Backbone Description: Backbone Size:3000; Vector Backbone:BS KS+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19272 Copy
Species: Mus musculus
Genetic Insert: mouse Wrn cDNA
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note: Plasmid contains a F1400L mutation compared to the NCBI reference sequence AAF64490.1. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_19273 Copy
Species: Mus musculus
Genetic Insert: 12.4kb Villin promoter
Vector Backbone Description: Backbone Size:2686; Vector Backbone:puc18; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: This is an updated version of the pBSII-12.4kbVillin promoter used in the cited publication. This vector is optimized with a larger, more useful MCS and easy linearization for transgenic injection. 12.4kb villin is a promoter to drive intestinal-specific expression of transgenes in the mouse intestinal epithelium. This construct was created by addition of two polylinker oligos between the HindIII and EcoR1 sites (replacing the MCS) in the puc18 vector, and then addition of the 12.4kb villin promoter (between EcoR1 and Sma1) and an SV40Pa (between HindIII and EcoRV) of the resulting MCS. Unique sites between the 12.4kb Villin promoter and SV40Pa are (from 5' to 3'): Sma1, Xho1, Mlu1, Cla1, SacII, Age1, Kpn1. Pre-transgene injection linearization of the plasmid can be obtained by digestion with PmeI, which removes the puc18 sequences.
Proper citation: RRID:Addgene_19358 Copy
Species: Mus musculus
Genetic Insert: formin1 iso1b
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_19320 Copy
Species: Mus musculus
Genetic Insert: FmnI Iso4 cDNA
Vector Backbone Description: Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19321 Copy
Species: Mus musculus
Genetic Insert: DnaJ (Hsp40) homolog, subfamily B, member 3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Part of the HSP40 gene family
Disclaimer:
For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene.
Proper citation: RRID:Addgene_19525 Copy
Species: Mus musculus
Genetic Insert: DNAJB10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Part of the HSP40 gene family
Disclaimer:
For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene.
Proper citation: RRID:Addgene_19507 Copy
Species: Mus musculus
Genetic Insert: neurogenin 3
Vector Backbone Description: Backbone Size:34864; Vector Backbone:pAd CMV/V5 DEST; Vector Types:Adenoviral, Gateway Cloning; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19410 Copy
Species: Mus musculus
Genetic Insert: DNAJB3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Part of the HSP40 gene family
Disclaimer:
For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene.
Proper citation: RRID:Addgene_19498 Copy
Species: Mus musculus
Genetic Insert: M33
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: (Kingston #1220)
Proper citation: RRID:Addgene_1951 Copy
Species: Mus musculus
Genetic Insert: pancreatic and duodenal homeobox 1
Vector Backbone Description: Backbone Size:34864; Vector Backbone:pAd CMV/V5 DEST; Vector Types:Adenoviral, Gateway Cloning; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19411 Copy
Species: Mus musculus
Genetic Insert: M33
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: (Kingston #1222)
Proper citation: RRID:Addgene_1952 Copy
Species: Mus musculus
Genetic Insert: neurogenic differentiation 1
Vector Backbone Description: Backbone Size:34864; Vector Backbone:pAd CMV/V5 DEST; Vector Types:Adenoviral, Gateway Cloning; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19414 Copy
Species: Mus musculus
Genetic Insert: 14-3-3 zeta
Vector Backbone Description: Backbone Size:6100; Vector Backbone:pEBG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_1942 Copy
Species: Mus musculus
Genetic Insert: DNAJB10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Part of the HSP40 gene family
Disclaimer:
For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene.
Proper citation: RRID:Addgene_19560 Copy
Species: Mus musculus
Genetic Insert: DNAJB10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Part of the HSP40 gene family
Disclaimer:
For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene.
Proper citation: RRID:Addgene_19533 Copy
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