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Species: E. coli
Genetic Insert: tdNfsB(F124W)-mCherry
Vector Backbone Description: Backbone Marker:Scott Sternson; Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30111097
Comments: Sternson Lab recommendations for propagation of AAV-FLEX plasmids: "These vectors are prone to recombination. This is a well known issue with these AAV vectors and is due to the inverted terminal repeats (ITRs) required for AAV production. To minimize recombination, we propagate these plasmids in NEB Stable cells. Also, to minimize recombination, cells should be cultured at 30 C.
Note that these cultures will grow slowly (20 h for minipreps). Better yields and culture times are obtained with 2xYT as the media. This is strongly recommended.
Because recombination may still happen occasionally, we do a panel of restriction digestions to assess whether the ITRs are in tact. Separate digestions with PvuII, Sma1, and SnaB1 should be performed."
Proper citation: RRID:Addgene_113762 Copy
Species: Homo sapiens
Genetic Insert: ZBTB12
Vector Backbone Description: Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pZBTB12-donor
Proper citation: RRID:Addgene_113769 Copy
Species: Homo sapiens
Genetic Insert: ZBTB12 homology arms flanking EGFP-IRES-Neo cassette
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Cloning source: Likely LCL.
Use with plasmid pZBTB12.1.0-gDNA
Proper citation: RRID:Addgene_113802 Copy
Species: Homo sapiens
Genetic Insert: ZNF561
Vector Backbone Description: Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pZNF561-donor
Proper citation: RRID:Addgene_113768 Copy
Species: P. putida
Genetic Insert: β subunit of Protocatechuate 3,4- Dioxygenase
Vector Backbone Description: Backbone Marker:DNASU plasmid repository, Arizona State University Biodesign Institute; Backbone Size:4010; Vector Backbone:pVP91A vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25548185
Comments: Please ensure that the chosen expression cell line expresses the T5 RNA polymerase.
Senavirathne, G., et al., Expression and purification of nuclease-free protocatechuate 3,4-dioxygenase for prolonged single-molecule fluorescence imaging. Anal Biochem, 2018. 556: p. 78-84.
Proper citation: RRID:Addgene_113766 Copy
Species: Homo sapiens
Genetic Insert: ZNF215 homology arms flanking EGFP-IRES-Neo cassette
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Cloning source: K562.
Use with plasmid pZNF215.1.0-gDNA
Proper citation: RRID:Addgene_113804 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: sup35 delta 2-5 mutant, 5' and 3' flanking regions
Vector Backbone Description: Backbone Size:4299; Vector Backbone:pRS306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10448860
Proper citation: RRID:Addgene_1138 Copy
Species: Homo sapiens
Genetic Insert: miR-30
Vector Backbone Description: Backbone Size:6963; Vector Backbone:MDH1-PGK-GFP 2.0; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14657504
Proper citation: RRID:Addgene_11379 Copy
Species: Mus musculus
Genetic Insert: miR-223
Vector Backbone Description: Backbone Size:6963; Vector Backbone:MDH1-PGK-GFP 2.0; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14657504
Proper citation: RRID:Addgene_11378 Copy
Species: Homo sapiens
Genetic Insert: PTCH1 (hedgehog dependent promoter)
Vector Backbone Description: Backbone Size:2775; Vector Backbone:pMuLE ENTR MCS R4-R3; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31860685
Comments: Winklmayr M, Schmid C, Laner-Plamberger S, Kaser A, Aberger F, Eichberger T, et al. Non-consensus GLI binding sites in Hedgehog target gene regulation. BMC molecular biology. 2010;11(1):2
Proper citation: RRID:Addgene_113864 Copy
Genetic Insert: CMV-eGFP
Vector Backbone Description: Backbone Size:9237; Vector Backbone:pMuLE Lenti Dest Neo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31860685
Proper citation: RRID:Addgene_113862 Copy
Species: Homo sapiens
Genetic Insert: ADP Ribosylation Factor 6
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16669702
Comments: Encodes a CFP fusion to the C-terminus of constitutively-inactive ARF6. CFP is the monomeric (A207K) version of ECFP.
Proper citation: RRID:Addgene_11386 Copy
Species: Homo sapiens
Genetic Insert: ADP Ribosylation Factor 1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16669702
Comments: Encodes a CFP fusion to the C-terminus of constitutively-active ARF1. CFP is the monomeric (A207K) version of ECFP.
Proper citation: RRID:Addgene_11385 Copy
Species: Homo sapiens
Genetic Insert: ADP Ribosylation Factor 6
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16669702
Comments: Encodes a CFP fusion to the C-terminus of ARF6. CFP is the monomeric (A207K) version of ECFP.
Proper citation: RRID:Addgene_11382 Copy
Species: Other
Genetic Insert: sfGFP-T2A-tdTomato
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_113853 Copy
Species: Other
Genetic Insert: sfGFP-T2A-tdTomato
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_113852 Copy
Species: Other
Genetic Insert: sfGFP-T2A-tdTomato
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_113851 Copy
Species: Other
Genetic Insert: sfGFP-T2A-tdTomato
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_113850 Copy
Species: Homo sapiens
Genetic Insert: T1R3
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:10393; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30723160
Proper citation: RRID:Addgene_113961 Copy
Species: Homo sapiens
Genetic Insert: T1R3
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:10393; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30723160
Proper citation: RRID:Addgene_113960 Copy
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