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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
aav-CAG-FLEX-tdNfsB(F124W)-mCherry (eNTR)
 
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RRID:Addgene_113762 tdNfsB(F124W)-mCherry E. coli Ampicillin PMID:30111097 Sternson Lab recommendations for propagation of AAV-FLEX plasmids: "These vectors are prone to recombination. This is a well known issue with these AAV vectors and is due to the inverted terminal repeats (ITRs) required for AAV production. To minimize recombination, we propagate these plasmids in NEB Stable cells. Also, to minimize recombination, cells should be cultured at 30 C. Note that these cultures will grow slowly (20 h for minipreps). Better yields and culture times are obtained with 2xYT as the media. This is strongly recommended. Because recombination may still happen occasionally, we do a panel of restriction digestions to assess whether the ITRs are in tact. Separate digestions with PvuII, Sma1, and SnaB1 should be performed." Backbone Marker:Scott Sternson; Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin F124W 2026-08-01 01:00:00 0
pZBTB12.1.0-gDNA
 
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RRID:Addgene_113769 ZBTB12 Homo sapiens Ampicillin gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pZBTB12-donor Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-01 01:00:00 0
pZBTB12-donor
 
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RRID:Addgene_113802 ZBTB12 homology arms flanking EGFP-IRES-Neo cassette Homo sapiens Ampicillin Cloning source: Likely LCL. Use with plasmid pZBTB12.1.0-gDNA Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin rs9267664, rs9267663 2026-08-01 01:00:01 0
pZNF561.1.0-gDNA
 
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RRID:Addgene_113768 ZNF561 Homo sapiens Ampicillin gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pZNF561-donor Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-01 01:00:00 0
pVP91A-pcaHG
 
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1+ mentions
RRID:Addgene_113766 β subunit of Protocatechuate 3,4- Dioxygenase P. putida Ampicillin PMID:25548185 Please ensure that the chosen expression cell line expresses the T5 RNA polymerase. Senavirathne, G., et al., Expression and purification of nuclease-free protocatechuate 3,4-dioxygenase for prolonged single-molecule fluorescence imaging. Anal Biochem, 2018. 556: p. 78-84. Backbone Marker:DNASU plasmid repository, Arizona State University Biodesign Institute; Backbone Size:4010; Vector Backbone:pVP91A vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:00:00 2
pZNF215-donor
 
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RRID:Addgene_113804 ZNF215 homology arms flanking EGFP-IRES-Neo cassette Homo sapiens Ampicillin Cloning source: K562. Use with plasmid pZNF215.1.0-gDNA Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin rs2239733, rs2239732, hg38:11:6956348:T>G, rs149189074, rs2239730, hg38:11:6956685:T>A 2026-08-01 01:00:01 0
pJLI-SUP35 (delta)2-5
 
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RRID:Addgene_1138 sup35 delta 2-5 mutant, 5' and 3' flanking regions Saccharomyces cerevisiae Ampicillin PMID:10448860 Backbone Size:4299; Vector Backbone:pRS306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin last four repeats of sup35 N domain deleted, but rest of ORF intact. Also includes sup35 5' and 3' flanking regions 2026-08-01 01:00:01 0
MDH1-miR-30-PGK-GFP
 
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1+ mentions
RRID:Addgene_11379 miR-30 Homo sapiens Ampicillin PMID:14657504 Backbone Size:6963; Vector Backbone:MDH1-PGK-GFP 2.0; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin 2026-08-01 01:00:01 1
MDH1-miR-223-PGK-GFP
 
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RRID:Addgene_11378 miR-223 Mus musculus Ampicillin PMID:14657504 Backbone Size:6963; Vector Backbone:MDH1-PGK-GFP 2.0; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin 2026-08-01 01:00:00 0
pMuLE_ENTR_PTCH1-mTurquoise2_R4-R3
 
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RRID:Addgene_113864 PTCH1 (hedgehog dependent promoter) Homo sapiens Kanamycin PMID:31860685 Winklmayr M, Schmid C, Laner-Plamberger S, Kaser A, Aberger F, Eichberger T, et al. Non-consensus GLI binding sites in Hedgehog target gene regulation. BMC molecular biology. 2010;11(1):2 Backbone Size:2775; Vector Backbone:pMuLE ENTR MCS R4-R3; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin 2026-08-01 01:00:02 0
pMuLE_EXPR_CMV-eGFP_TOP-NLuc1.1_12GLI-FLuc_CBF-GLuc
 
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1+ mentions
RRID:Addgene_113862 CMV-eGFP Ampicillin PMID:31860685 Backbone Size:9237; Vector Backbone:pMuLE Lenti Dest Neo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-01 01:00:02 2
pARF6(T27N)-CFP
 
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1+ mentions
RRID:Addgene_11386 ADP Ribosylation Factor 6 Homo sapiens Kanamycin PMID:16669702 Encodes a CFP fusion to the C-terminus of constitutively-inactive ARF6. CFP is the monomeric (A207K) version of ECFP. Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin Changed Threonine 27 to Asparagine. 2026-08-01 01:00:02 7
pARF1(Q71L)-CFP
 
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1+ mentions
RRID:Addgene_11385 ADP Ribosylation Factor 1 Homo sapiens Kanamycin PMID:16669702 Encodes a CFP fusion to the C-terminus of constitutively-active ARF1. CFP is the monomeric (A207K) version of ECFP. Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin Changed Glutamine 71 to Leucine. 2026-08-01 01:00:01 1
pARF6-CFP
 
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10+ mentions
RRID:Addgene_11382 ADP Ribosylation Factor 6 Homo sapiens Kanamycin PMID:16669702 Encodes a CFP fusion to the C-terminus of ARF6. CFP is the monomeric (A207K) version of ECFP. Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-01 01:00:01 10
CAG-loxP-3xpA-sfGFP-tdTomato T2A V XE
 
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RRID:Addgene_113853 sfGFP-T2A-tdTomato Other Ampicillin Vector Backbone:Custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:00:02 0
CAG-loxP-3xpA-sfGFP-tdTomato T2A Q XE
 
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RRID:Addgene_113852 sfGFP-T2A-tdTomato Other Ampicillin Vector Backbone:Custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:00:02 0
CAG-loxP-3xpA-sfGFP-tdTomato T2A APGST XE
 
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RRID:Addgene_113851 sfGFP-T2A-tdTomato Other Ampicillin Vector Backbone:Custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:00:01 0
CAG-loxP-3xpA-sfGFP-tdTomato T2A APGST -XE
 
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RRID:Addgene_113850 sfGFP-T2A-tdTomato Other Ampicillin Vector Backbone:Custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:00:01 0
pCEP4-HLA-c-myc-optiT1R3 ECD-MHC
 
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RRID:Addgene_113961 T1R3 Homo sapiens Ampicillin PMID:30723160 Backbone Marker:invitrogen; Backbone Size:10393; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin residues 21-563 fused to a transmembrane tether 2026-08-01 01:00:03 0
pCEP4-HLA-c-myc-optiT1R3 ECD
 
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RRID:Addgene_113960 T1R3 Homo sapiens Ampicillin PMID:30723160 Backbone Marker:invitrogen; Backbone Size:10393; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin residues 21-563 2026-08-01 01:00:03 0

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