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http://www.addgene.org/166747

Species: Mus musculus
Genetic Insert: tBid-2E
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: BH3 region has hydrophobic positions H1 & H2 mutated to glutamic acid (E). Mutations: I27E, L31E

Proper citation: RRID:Addgene_166747 Copy   

•   Source: Addgene

http://www.addgene.org/16689

Species: Mus musculus
Genetic Insert: Frizzled-8
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This is the CRD domain of mouse Frizzled-8 as an IgG fusion.

Proper citation: RRID:Addgene_16689 Copy   

•   Source: Addgene

http://www.addgene.org/166954

Species: Mus musculus
Genetic Insert: KIF5A
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_166954 Copy   

•   Source: Addgene

http://www.addgene.org/166952

Species: Mus musculus
Genetic Insert: KIF5A
Vector Backbone Description: Vector Backbone:pLV-eF1a-IRES::Puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_166952 Copy   

•   Source: Addgene

http://www.addgene.org/166958

Species: Mus musculus
Genetic Insert: KIF5A
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_166958 Copy   

•   Source: Addgene

http://www.addgene.org/166965

Species: Mus musculus
Genetic Insert: KLC1
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_166965 Copy   

•   Source: Addgene

http://www.addgene.org/166964

Species: Mus musculus
Genetic Insert: KLC1
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_166964 Copy   

•   Source: Addgene

http://www.addgene.org/166963

Species: Mus musculus
Genetic Insert: KLC1-N343S
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_166963 Copy   

•   Source: Addgene

http://www.addgene.org/16691

Species: Mus musculus
Genetic Insert: sFRP-3
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_16691 Copy   

•   Source: Addgene

http://www.addgene.org/16701

Species: Mus musculus
Genetic Insert: CBP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5200; Vector Backbone:pRc/RSV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: HA-tagged mouse CREB-binding-protein.

Proper citation: RRID:Addgene_16701 Copy   

•   Source: Addgene

http://www.addgene.org/167344

Species: Mus musculus
Genetic Insert: Zinc finger matrin-type 3
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_167344 Copy   

•   Source: Addgene

http://www.addgene.org/16730

Species: Mus musculus
Genetic Insert: mouse Casein Kinase II alpha-pCS2P+
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2P+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Description: Subcloned from XE 163 into Stu/Xba sites of CS2P+ Linearize with ASP718 and transcribe SP6. Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT. Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes. Development. 2004 Dec;131(23):5909-21.

Proper citation: RRID:Addgene_16730 Copy   

•   Source: Addgene

http://www.addgene.org/167503

Species: Mus musculus
Genetic Insert: Ppp1r15b
Vector Backbone Description: Backbone Size:2773; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_167503 Copy   

•   Source: Addgene

http://www.addgene.org/167573

Species: Mus musculus
Genetic Insert: Ribo-GCaMP8f
Vector Backbone Description: Backbone Size:2868; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_167573 Copy   

•   Source: Addgene

http://www.addgene.org/167572

Species: Mus musculus
Genetic Insert: Ribo-GCaMP8s
Vector Backbone Description: Backbone Size:2868; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_167572 Copy   

•   Source: Addgene

http://www.addgene.org/167854

Species: Mus musculus
Genetic Insert: sgRNA targeting Zmat3
Vector Backbone Description: Vector Backbone:pLL3.3; Vector Types:Mammalian Expression, Mouse Targeting, Lentiviral, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_167854 Copy   

•   Source: Addgene

http://www.addgene.org/41183

Species: Mus musculus
Genetic Insert: Cytochrome C-GFP
Vector Backbone Description: Vector Backbone:pBabe (puro); Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_41183 Copy   

•   Source: Addgene

http://www.addgene.org/41182

Species: Mus musculus
Genetic Insert: Cytochrome C
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_41182 Copy   

•   Source: Addgene

http://www.addgene.org/41382

Species: Mus musculus
Genetic Insert: RIP3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_41382 Copy   

•   Source: Addgene

http://www.addgene.org/41663

Species: Mus musculus
Genetic Insert: Mus musculus GABA transporter 1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: To generate the fluorescent mutants mGAT10XFP and mGAT1XFP* through mGAT1XFP45, the wild-type mGAT1 open reading frame (ORF) was subcloned without its original stop codon into the HindIII and EcoRI sites of the pcDNA3.1(+) expression vector multiple cloning site (MCS). XFP ORFs were then subcloned downstream from and in frame with the mGAT1 ORF at the NotI and XbaI sites of the pcDNA3.1(+) MCS. This resulted in a 12–amino acid spacer between the end of the mGAT1 sequence and the beginning of the fluorophore. The depositors modified a method for the integration of PCR fragments without the use of restriction enzymes (Geiser et al., 2001) to add the final 3, 8, 20, 28, or 45 codons of the human GAT1 (hGAT1) ORF. These were amplified from a source plasmid using the proof-reading PfuTurbo Cx Hotstart polymerase with 5′ and 3′ extensions corresponding to the 20–22-nt regions that flanked the intended site of insertion, such that the PCR product integrated in-frame immediately after the fluorophore sequence when used as the primers in a subsequent QuikChange II XL mutagenesis PCR reaction. For mGAT1XFP*, the depositors simply added a GTC codon for Val after the fluorophore ORF. The attached image displays the protein sequences of the modified regions of mGAT1 for each fluorescent construct. mGAT10CFP and mGAT10YFP repeated the fusion design of mGAT10GFP but with the fluorophore exchanged as annotated. The three C-terminal residues of the mGAT0XFP fusions are -YKI-CO2−, which comprises a broadly defined consensus PDZ class II–interacting motif (X-φ-X-φ, where φ designates a hydrophobic residue and X any residue) (Sheng and Sala, 2001; Hung and Sheng, 2002). The depositors searched the Ensembl databases using Biomart (http://www.biomart.org/) (Spudich et al., 2007) and applied the GO:0005886 “plasma membrane” cellular component filter. The search identified no known membrane proteins possessing the -YKI-CO2− C-terminal sequence. In the mGAT1XFP* constructs, the depositors defined the terminal residue P(0) more narrowly, changing the terminal isoleucine residue present in mGAT10XFP to a valine in mGAT1XFP*. The resulting C-terminal sequence, -YKV-CO2−, reconstituted a functional PDZ class II–interacting motif present in Ephrin B receptors, a class that relies on interactions with the PDZ domain–containing proteins for clustering (Torres et al., 1998; Brückner et al., 1999; Lin et al., 1999; Madsen et al., 2005). Other constructs in the C-terminal XFP fusion series, mGAT1XFP3, mGAT1XFP8, mGAT1XFP20, mGAT1XFP28, and mGAT1XFP45, had the most C-terminal 3, 8, 20, 28, or 45 residues of the hGAT1 appended after the mGAT1XFP fusion. The differences in nucleotide sequence between the hGAT1 and mGAT1 C termini were a useful source of positive identification when the depositors analyzed the clones during construction. PCR integration was applied to amplify and insert EYFP or ECFP directly between residues R565 and L566, I570 and Q571, or V577 and R578 of mGAT1 to generate the mGAT15xxXFP5xxCT constructs. The site of XFP insertion in GAT1 is highlighted in the nomenclatures for these constructs by residue numbers flanking the fluorophore, and the “CT” denotes that the insertion occurs within the C terminus. Please see the associated article for more detailed information regarding construct creation and usage.

Proper citation: RRID:Addgene_41663 Copy   

•   Source: Addgene