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Species: Mus musculus
Genetic Insert: p21
Vector Backbone Description: Backbone Marker:Ivanova, NB; Backbone Size:10130; Vector Backbone:FUGW-H1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid targets the open reading frame of p21. The 19mer target sequence is: AGTAGCAGTTGTACAAGGA
Proper citation: RRID:Addgene_25869 Copy
Species: Mus musculus
Genetic Insert: Gq alpha
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Gz alpha residues (EYNLV to YIGLC). Works the same as qi5 and is the least popular since no one knows what Gz alpha really does in nature. Since qz5 is not sensitive to pertussis toxin, qz5 may be the only G protein activated by a Gi-coupled receptor in cells treated with pertussis toxin. This trick can be experimentally useful in settings where you want experimental control of the exact G protein and the receptor that is activated. It is theoretically possible that this construct will work better than the other constructs for particular receptors, but the Conklin lab has not seen this happen yet.
Contains an internal hemaglutinin epitope tag (DVPDYA) that has no effect on receptor coupling.
Please see supplemental document for G-protein chimera user manual.
Proper citation: RRID:Addgene_25867 Copy
Species: Mus musculus
Genetic Insert: Gata3
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2997; Vector Backbone:pGEM-7zf(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_25796 Copy
Species: Mus musculus
Genetic Insert: Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc
Vector Backbone Description: Backbone Size:10600; Vector Backbone:mCol.loxneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_25794 Copy
Species: Mus musculus
Genetic Insert: twisted gastrulation
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Xenopus chordin signal peptide N-epitope. Will homo-dimerize, binds covalently to Chordin and is recognized by R&D monoclonal anti-TSG antibody MAB756. Prevents the making of Ternary complex as published in Oelgeschlager M, et al., 2003b. Purified protein is readily ventralizing when injected in blastocoel.
Proper citation: RRID:Addgene_25778 Copy
Species: Mus musculus
Genetic Insert: cross veinless 2
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: For sense, linearize with NotI, transcribe with SP6.
Protein C Epitope tag sequence - EDQVDPRLIDGK
Proper citation: RRID:Addgene_25776 Copy
Species: Mus musculus
Genetic Insert: Mfn1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(-)/Myc-His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_26050 Copy
Species: Mus musculus
Genetic Insert: MyoD-E12 Heterodimer Fusion
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Baculovirus transfer Vector; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_25990 Copy
Species: Mus musculus
Genetic Insert: MyoD-MyoD homodimer
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Baculovirus transfer vector; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_25992 Copy
Species: Mus musculus
Genetic Insert: Mfn2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(-)/Myc-His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_26051 Copy
Species: Mus musculus
Genetic Insert: Kinase suppressor of Ras 1
Vector Backbone Description: Backbone Size:6500; Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_25979 Copy
Species: Mus musculus
Genetic Insert: Kinase suppressor of Ras 1
Vector Backbone Description: Backbone Size:6500; Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_25977 Copy
Species: Mus musculus
Genetic Insert: Kinase suppressor of Ras 1
Vector Backbone Description: Backbone Size:6500; Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_25973 Copy
Species: Mus musculus
Genetic Insert: spot14
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGEX; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_25980 Copy
Species: Mus musculus
Genetic Insert: MyoD(QQQ)-E12 Heterodimer Fusion
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Baculovirus transfer vector; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_26003 Copy
Species: Mus musculus
Genetic Insert: Synaptophysin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7500; Vector Backbone:pBi; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: There are 6 mismatches between author sequence and Addgene sequence but they do not alter the amino acid sequence.
Proper citation: RRID:Addgene_26121 Copy
Species: Mus musculus
Genetic Insert: Synaptophysin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4300; Vector Backbone:pBi; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_26084 Copy
Species: Mus musculus
Genetic Insert: mCD8::GFP
Vector Backbone Description: Backbone Size:7773; Vector Backbone:pJFRC-MUH; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_26218 Copy
Species: Mus musculus
Genetic Insert: mCD8::GFP
Vector Backbone Description: Backbone Size:7768; Vector Backbone:pJFRC-MUH; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_26217 Copy
Species: Mus musculus
Genetic Insert: mCD8::GFP
Vector Backbone Description: Backbone Size:7901; Vector Backbone:pJFRC-MUH; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_26214 Copy
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