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Species: Homo sapiens
Genetic Insert: Rb
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Full length Rb coding sequence, amino acids 1-928. The Rb sequence comprised between sites BamHI and SalI was cloned between BglII and SalI sites in pEGFP-C1.
Proper citation: RRID:Addgene_16004 Copy
Species: Homo sapiens
Genetic Insert: Akt2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5600; Vector Backbone:pcDNA3.1(+)/Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: There is a silent mutation at Arg552 (CGA to CGG).
Proper citation: RRID:Addgene_16000 Copy
Species: Homo sapiens
Genetic Insert: CMV-OsTIR1(F74A)
Vector Backbone Description: Vector Backbone:pMK359; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_159992 Copy
Species: Homo sapiens
Genetic Insert: FLIP
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: A 1,460-bp sequence containing the promoter region of the FLIP gene was amplified using the BAC clone RP11-536118 (Children’s Hospital Oakland Research Institute, Oakland, Calif.) and inserted into the luciferase-expressing reporter plasmid pGL3-basic (Promega). The sequence corresponds to positions –1179 to +281 relative to the proposed transcriptional start site of CFLAR (FLIP) exon 1 (accession no. AB038965).
Proper citation: RRID:Addgene_16016 Copy
Species: Homo sapiens
Genetic Insert: CARP1
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_16014 Copy
Species: Homo sapiens
Genetic Insert: CARP2
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_16013 Copy
Species: Homo sapiens
Genetic Insert: KILLER/DR5
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5600; Vector Backbone:pGL2-basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: A 3.6 Kb Pst1 fragment from the KILLER/DR5 P1 clone which includes 1.6 Kb upstream of the ATG through Intron 2 in KILLER/DR5 genomic DNA.
Proper citation: RRID:Addgene_16012 Copy
Species: Homo sapiens
Genetic Insert: cmyc
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Contains cmyc with Kozak.
Proper citation: RRID:Addgene_16011 Copy
Species: Homo sapiens
Genetic Insert: ARHGEF9
Vector Backbone Description: Vector Backbone:mu6pro; Vector Types:RNAi; Bacterial Resistance:Ampicillin
References:
Comments: Targets collybistin MTL-CBSH3- mRNA (target is located in the protein coding region. Putative rat sequence based on human sequence - targets nucleotides 813-834 of human NM_001353924.2. Targets MTL collybistin isoforms without SH3 domain). There is also the mutated shRNA control for this isoform (Addgene #160211).
Proper citation: RRID:Addgene_160210 Copy
Species: Homo sapiens
Genetic Insert: PAK5
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pCMV6-myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Order of elements: SalI-Myc-XbaI-Pak5-EcoRI
Proper citation: RRID:Addgene_16020 Copy
Species: Homo sapiens
Genetic Insert: COL4A1
Vector Backbone Description: Backbone Marker:Clonetech; Vector Backbone:EGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Please note: Plasmid contains a T824P mutation in the insert (lies within the Mature Domain). This variant is not known to affect plasmid function.
Proper citation: RRID:Addgene_160317 Copy
Species: Homo sapiens
Genetic Insert: α5first concatemer
Vector Backbone Description: Backbone Size:4668; Vector Backbone:pKR5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_160269 Copy
Species: Homo sapiens
Genetic Insert: methyltransferase like 3
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_160250 Copy
Species: Homo sapiens
Genetic Insert: methyltransferase like 3
Vector Backbone Description: Backbone Size:6900; Vector Backbone:ePB-PURO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_160252 Copy
Species: Homo sapiens
Genetic Insert: beta4
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_16038 Copy
Species: Homo sapiens
Genetic Insert: alpha6
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Addgene's sequencing results indicate that this plasmid contains alpha-6 integrin isoform b.
Proper citation: RRID:Addgene_16036 Copy
Species: Homo sapiens
Genetic Insert: c-Fyn
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_16035 Copy
Species: Homo sapiens
Genetic Insert: c-Fyn
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_16034 Copy
Species: Homo sapiens
Genetic Insert: c-Fyn
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_16032 Copy
Species: Homo sapiens
Genetic Insert: GPR143
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pLJC5; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_160368 Copy
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