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Species: Homo sapiens
Genetic Insert: ubh12.672.793
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7328; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3FL2. http://www.thesgc.org/structures/3FL2/
Proper citation: RRID:Addgene_25359 Copy
Species: Homo sapiens
Genetic Insert: RNF128.038.204
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:0; Vector Backbone:pFHMSP-LIC-N; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: PDB: 3ICU. http://www.thesgc.org/structures/3ICU/
Proper citation: RRID:Addgene_25356 Copy
Species: Homo sapiens
Genetic Insert: LRRK2
Vector Backbone Description: Backbone Marker:Cookson Lab; Backbone Size:4300; Vector Backbone:pCMV-Tag 3B; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18397888
Comments: An additional Myc tag has been added to the original vector. The 2XMyc vector was adapted to the invitrogen gateway system by insertion of a gateway cloning cassette. LRRK2 was cloned by gateway recombination.
In order to provide these LRRK2 plasmids as a resource to the community, we depend on the contributing academic laboratory for quality control. Therefore, the full-length LRRK2 plasmids are distributed in the format provided by the contributing institution "as is" with no additional guarantee.
Addgene NGS detected additional S212L and I1122V mutations in this plasmid relative to AAV63975.1.
Addgene is not responsible for any errors in these constructs.
It is strongly encouraged that a diagnostic digest be performed on this plasmid before use in an experiment (see attached PDF).
If there is an issue with your stock, please contact the Cookson lab for a replacement.
Proper citation: RRID:Addgene_25366 Copy
Species: Homo sapiens
Genetic Insert: LRRK2
Vector Backbone Description: Backbone Marker:Cookson Lab; Backbone Size:4300; Vector Backbone:pCMV-Tag 3B; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18397888
Comments: An additional Myc tag has been added to the original vector. The 2XMyc vector was adapted to the invitrogen gateway system by insertion of a gateway cloning cassette. LRRK2 was cloned by gateway recombination.
In order to provide these LRRK2 plasmids as a resource to the community, we depend on the contributing academic laboratory for quality control. Therefore, the full-length LRRK2 plasmids are distributed in the format provided by the contributing institution "as is" with no additional guarantee.
Addgene is not responsible for any errors in these constructs.
It is strongly encouraged that a diagnostic digest be performed on this plasmid before use in an experiment (see attached PDF).
If there is an issue with your stock, please contact the Cookson lab for a replacement.
Proper citation: RRID:Addgene_25364 Copy
Species: Homo sapiens
Genetic Insert: ECT2:V237-E330
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3L46. http://www.thesgc.org/structures/3L46/
Proper citation: RRID:Addgene_25338 Copy
Species: Homo sapiens
Genetic Insert: CENTA1:M1-P374
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28GST-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3FM8. http://www.thesgc.org/structures/3FM8/
Proper citation: RRID:Addgene_25334 Copy
Species: Homo sapiens
Genetic Insert: GIMAP1:E25-R253
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: There is a V196E amino acid change compared to NCBI sequence for 3LXW.
PDB: 3LXW. http://www.thesgc.org/structures/3LXW/
Proper citation: RRID:Addgene_25336 Copy
Species: Homo sapiens
Genetic Insert: CBX6_02
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7328; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3GV6. http://www.thesgc.org/structures/3GV6/
Proper citation: RRID:Addgene_25296 Copy
Species: Homo sapiens
Genetic Insert: USP5.001.835
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7328; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3IHP. http://www.thesgc.org/structures/3IHP/
Proper citation: RRID:Addgene_25299 Copy
Species: Homo sapiens
Genetic Insert: rnf168.0001.0113
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7328; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3L11. http://www.thesgc.org/structures/3L11/
Proper citation: RRID:Addgene_25298 Copy
Species: Homo sapiens
Genetic Insert: RALGDS:N793-F914
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3KH0. http://www.thesgc.org/structures/3KH0/
Proper citation: RRID:Addgene_25331 Copy
Species: Homo sapiens
Genetic Insert: BAT8_08
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7328; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3K5K. http://www.thesgc.org/structures/3K5K/
Proper citation: RRID:Addgene_25295 Copy
Species: Homo sapiens
Genetic Insert: SETD2
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3H6L. http://www.thesgc.org/structures/3H6L/
Proper citation: RRID:Addgene_25348 Copy
Species: Homo sapiens
Genetic Insert: hecw1.0900.1035
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3L4H. http://www.thesgc.org/structures/3L4H/
Proper citation: RRID:Addgene_25347 Copy
Species: Homo sapiens
Genetic Insert: herc2.1203.1296
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 2KEO. http://www.thesgc.org/structures/2KEO/
Proper citation: RRID:Addgene_25346 Copy
Species: Homo sapiens
Genetic Insert: FXR1_25
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3KUF. http://www.thesgc.org/structures/3KUF/
PDB: 3O8V. http://www.thesgc.org/structures/3O8V/
Proper citation: RRID:Addgene_25341 Copy
Species: Homo sapiens
Genetic Insert: epha8.0603.0909
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3KUL. http://www.thesgc.org/structures/3KUL/
Proper citation: RRID:Addgene_25343 Copy
Species: Homo sapiens
Genetic Insert: MPP8_4
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB:
https://www.thesgc.org/structures/3LWE/
https://www.thesgc.org/structures/6V2S/
Proper citation: RRID:Addgene_25316 Copy
Species: Homo sapiens
Genetic Insert: SMYD3_26
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3MEK. http://www.thesgc.org/structures/3MEK/
Proper citation: RRID:Addgene_25318 Copy
Species: Homo sapiens
Genetic Insert: SGF29_6
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3MEU. http://www.thesgc.org/structures/3MEU/
Proper citation: RRID:Addgene_25317 Copy
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