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Species: Homo sapiens
Genetic Insert: emiRFP703-tubulin
Vector Backbone Description: Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31932632
Proper citation: RRID:Addgene_136564 Copy
Species: Homo sapiens
Genetic Insert: HPV 16 E7 L67K
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4900; Vector Backbone:pGEX2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8445736
Proper citation: RRID:Addgene_13650 Copy
Species: Homo sapiens
Genetic Insert: vimentin-emiRFP703
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31932632
Proper citation: RRID:Addgene_136566 Copy
Species: Homo sapiens
Genetic Insert: H2B-emiRFP670
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31932632
Proper citation: RRID:Addgene_136571 Copy
Species: Homo sapiens
Genetic Insert: vimentin-emiRFP670
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31932632
Proper citation: RRID:Addgene_136572 Copy
Species: Homo sapiens
Genetic Insert: PDGFRbeta
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10381; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32559251
Proper citation: RRID:Addgene_136455 Copy
Species: Homo sapiens
Genetic Insert: NRF2
Vector Backbone Description: Backbone Marker:Addgene #1765; Backbone Size:5219; Vector Backbone:pBABE-hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32291314
Proper citation: RRID:Addgene_136579 Copy
Species: Homo sapiens
Genetic Insert: gRNA against human PLOD2
Vector Backbone Description: Backbone Size:9580; Vector Backbone:pSico; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34107376
Proper citation: RRID:Addgene_136459 Copy
Species: Homo sapiens
Genetic Insert: gRNA against human PLOD2
Vector Backbone Description: Backbone Size:9580; Vector Backbone:pSico; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34107376
Proper citation: RRID:Addgene_136460 Copy
Species: Homo sapiens
Genetic Insert: DNA Helicase B
Vector Backbone Description: Backbone Size:9136; Vector Backbone:CSII-EF-MCS; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24075009
Proper citation: RRID:Addgene_136461 Copy
Species: Homo sapiens
Genetic Insert: HPV 16 E7 Q mutant
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4900; Vector Backbone:pGEX2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8445736
Proper citation: RRID:Addgene_13648 Copy
Species: Homo sapiens
Genetic Insert: HPV 16 E7 L67R
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4900; Vector Backbone:pGEX2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8445736
Proper citation: RRID:Addgene_13649 Copy
Species: Homo sapiens
Genetic Insert: NRF2(1584A>C,1586A>T,1589A>G)
Vector Backbone Description: Backbone Marker:Addgene #83274; Backbone Size:4422; Vector Backbone:pEN_TT miRc2 3xFLAG; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:32291314
Proper citation: RRID:Addgene_136522 Copy
Species: Homo sapiens
Genetic Insert: p53(R280K)
Vector Backbone Description: Backbone Marker:Addgene #25752; Backbone Size:4422; Vector Backbone:pEN_TTmiRc2; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:32291314
Proper citation: RRID:Addgene_136525 Copy
Species: Homo sapiens
Genetic Insert: CHEK2
Vector Backbone Description: Backbone Marker:Addgene #83274; Backbone Size:4496; Vector Backbone:pEN_TT miRc2 3xFLAG; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:32291314
Proper citation: RRID:Addgene_136526 Copy
Species: Homo sapiens
Genetic Insert: NRF2
Vector Backbone Description: Backbone Marker:Addgene #83274; Backbone Size:4496; Vector Backbone:pEN_TT miRc2 3xFLAG; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:32291314
Proper citation: RRID:Addgene_136527 Copy
Species: Homo sapiens
Genetic Insert: CHEK2
Vector Backbone Description: Backbone Marker:Addgene #25735; Backbone Size:13290; Vector Backbone:pSLIK-Neo; Vector Types:Mammalian Expression, Lentiviral, destinatioin vector for gateway cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32291314
Proper citation: RRID:Addgene_136536 Copy
Species: Homo sapiens
Genetic Insert: Beclin-1 IDR + CC
Vector Backbone Description: Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29185708
Proper citation: RRID:Addgene_136686 Copy
Species: Homo sapiens
Genetic Insert: ZNF8
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: K562
Proper citation: RRID:Addgene_136689 Copy
Species: Homo sapiens
Genetic Insert: Beclin-1
Vector Backbone Description: Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29185708
Comments: Large-scale expression of the BECN1 fusion protein was performed by growing cultures in TB at 37 °C with orbital shaking at 180 rpm to mid-log phase (A600 ∼ 0.6−0.8), cooling cultures to 18 °C for 30 min before induction with 0.5 mM isopropyl β-D-1-thiogalactopyranoside, and growing the induced cultures for 18 h at 18 °C. Cell pellets were harvested via centrifugation and stored at −50 °C.
See Ranaghan et al. (2017) [DOI: 10.1021/acs.biochem.7b00936] for purification methods.
Proper citation: RRID:Addgene_136688 Copy
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