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Species: Mus musculus
Genetic Insert: FGFR1 kinase, FKBP12v36
Vector Backbone Description: Backbone Marker:GR Crabtree lab; Backbone Size:3500; Vector Backbone:pSH1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_15285 Copy
Species: Mus musculus
Genetic Insert: delPH-Akt
Vector Backbone Description: Backbone Marker:GR Crabtree lab; Backbone Size:3500; Vector Backbone:pSH1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_15288 Copy
Species: Mus musculus
Genetic Insert: FGFR2 kinase, FKBP12v36
Vector Backbone Description: Backbone Marker:GR Crabtree lab; Backbone Size:3500; Vector Backbone:pSH1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_15286 Copy
Species: Mus musculus
Genetic Insert: PRKACB
Vector Backbone Description: Backbone Marker:Dr. Richard Palmiter; Backbone Size:4100; Vector Backbone:Zem3 (pUC13 + MT prom + hGH polyA); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Zem3 is a pUC13 derivative that contains a mouse metallothionein-I promoter and the human growth hormone pol(A) region separated by a BglII restriction site. pCbeta EV was constructed by isolating the 1246bp SacI fragment of pMCbeta and inserting it into the BglII site of Zem3 after ligation of BamHI linkers onto the cDNA fragment.
Proper citation: RRID:Addgene_15311 Copy
Species: Mus musculus
Genetic Insert: murine CD3 delta-gamma-epsilon-zeta subunits
Vector Backbone Description: Backbone Marker:Maki Nakayama; Vector Backbone:pMI-LO; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: Second gene is LSSmOrange
Proper citation: RRID:Addgene_153418 Copy
Species: Mus musculus
Genetic Insert: murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2)
Vector Backbone Description: Backbone Marker:Maki Nakayama; Vector Backbone:pMI-Puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: Second gene is puromycin-resistant gene
Proper citation: RRID:Addgene_153420 Copy
Species: Mus musculus
Genetic Insert: Chr2-GFP-P2A-GFP
Vector Backbone Description: Vector Backbone:AAV-Dlx-GFP; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/808170v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_153437 Copy
Species: Mus musculus
Genetic Insert: Chr2-GFP-P2A-GFP
Vector Backbone Description: Vector Backbone:AAV-Dlx-GFP; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/808170v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_153434 Copy
Species: Mus musculus
Genetic Insert: Hes7
Vector Backbone Description: Backbone Size:2974; Vector Backbone:pBluescript; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_153528 Copy
Species: Mus musculus
Genetic Insert: Fusion of vhhGPF4 nanobody (intracellular) with mouse CD8 transmembrane protein and mCherry fluorophor
Vector Backbone Description: Backbone Marker:Kanca, O. et.al., Raeppli: a whole-tissue labeling tool for live imaging of Drosophila development. Development (2014); Backbone Size:8900; Vector Backbone:pUASTLOTattB; Vector Types:Insect Expression, Cre/Lox; Bacterial Resistance:Ampicillin
References:
Comments: pUASTLOTattB vector:
Kanca, O., Caussinus, E., Denes, A. S., Percival-Smith, A. & Affolter, M. Raeppli: a
whole-tissue labeling tool for live imaging of Drosophila development.
Development 141, 472–480 (2014).
vhhGFP4 plasmid:
Saerens D, Pellis M, Loris R, Pardon E, Dumoulin M, Matagne A, Wyns L, Muyldermans S, Conrath K
J Mol Biol. 2005 Sep 23; 352(3):597-607.
mCD8 plasmid:
Lee, T. & Luo, L. Mosaic analysis with a repressible cell marker for studies of
gene function in neuronal morphogenesis. Neuron 22, 451–461 (1999).
mCherry: Clonetech
Proper citation: RRID:Addgene_163930 Copy
Species: Mus musculus
Genetic Insert: kif13b
Vector Backbone Description: Backbone Marker:thermo fisher (Invitrogen); Backbone Size:4810; Vector Backbone:pFastBac HTa; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_163908 Copy
Species: Mus musculus
Genetic Insert: kif13b
Vector Backbone Description: Backbone Marker:thermo fisher (Invitrogen); Backbone Size:4812; Vector Backbone:pFastBac HTa; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_163907 Copy
Species: Mus musculus
Genetic Insert: Fusion of vhhGPF4 nanobody with mouse CD8 transmembrane protein and mCherry fluorophor
Vector Backbone Description: Backbone Marker:Kanca, O. et.al., Raeppli: a whole-tissue labeling tool for live imaging of Drosophila development. Development (2014); Backbone Size:8900; Vector Backbone:pUASTLOTattB; Vector Types:Insect Expression, Cre/Lox; Bacterial Resistance:Ampicillin
References:
Comments: For pUASTLOTattB_VHH-GFP4::CD8::mCherry, we inserted the VHH-GFP4 fragment after the signal peptide sequence of the mouse CD8 domain in the pUAS::CD8::GFP plasmid. We replaced the GFP by a mCherry (Clonetech) and finally cloned the VHH-GFP4::CD8::mCherry fragment into the pUASTLOTattB vector.
pUASTLOTattB vector:
Kanca, O., Caussinus, E., Denes, A. S., Percival-Smith, A. & Affolter, M. Raeppli: a
whole-tissue labeling tool for live imaging of Drosophila development.
Development 141, 472–480 (2014).
vhhGFP4 plasmid:
Saerens D, Pellis M, Loris R, Pardon E, Dumoulin M, Matagne A, Wyns L, Muyldermans S, Conrath K
J Mol Biol. 2005 Sep 23; 352(3):597-607.
mCD8 plasmid:
Lee, T. & Luo, L. Mosaic analysis with a repressible cell marker for studies of
gene function in neuronal morphogenesis. Neuron 22, 451–461 (1999).
mCherry: Clonetech
Proper citation: RRID:Addgene_163917 Copy
Species: Mus musculus
Genetic Insert: MeCP2 R106W
Vector Backbone Description: Vector Backbone:pAAV-EF1a; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164063 Copy
Species: Mus musculus
Genetic Insert: MeCP2 Isoform 1 R133C
Vector Backbone Description: Vector Backbone:pAAV-EF1a; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164064 Copy
Species: Mus musculus
Genetic Insert: Hq-ERV
Vector Backbone Description: Backbone Marker:Invitrogen/ThermoFisher; Backbone Size:3923; Vector Backbone:pCR-XL-2-TOPO; Vector Types:eukaryotic expression vector; Bacterial Resistance:Ampicillin
References:
Comments: The C1-20 insert was derived from the pcDNA-C120-empty plasmid (Motta-Mena and Gardner) and introduced into pTOPO-HqERV (Addgene plasmid #164088) to generate the current plasmid, pTOPO-HqERV(C1-20).
Proper citation: RRID:Addgene_164090 Copy
Species: Mus musculus
Genetic Insert: anti-TRIP8b (Rattus norvegicus) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Isotype: IgG2a.
A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_164154 Copy
Species: Mus musculus
Genetic Insert: pBS-Sema3C
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:In Situ Template; Bacterial Resistance:Ampicillin
References:
Comments: Antisense probes are generated by digesting with NotI and transcribing with T3 RNA Polymerase.
Proper citation: RRID:Addgene_16426 Copy
Species: Mus musculus
Genetic Insert: (Cyto)iGlucoSnFR-mRuby2
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments: The depositing lab recommends the updated pAAV.hSynap.(cyto).iGlucoSnFR2.mRuby3 (https://www.addgene.org/244092/) instead of this plasmid.
Please visit https://doi.org/10.1101/571422 for bioRxiv preprint.
Proper citation: RRID:Addgene_164510 Copy
Species: Mus musculus
Genetic Insert: CD200
Vector Backbone Description: Backbone Marker:Jeng-Shin Lee, Harvard University; Backbone Size:4768; Vector Backbone:AAV-MCS8; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164424 Copy
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