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Species: Homo sapiens
Genetic Insert: His6-tev-Hs.CALM1(2-148)
Vector Backbone Description: Vector Backbone:pDest-527; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_159693 Copy
Species: Homo sapiens
Genetic Insert: Hs.KRAS4b(1-169)
Vector Backbone Description: Vector Backbone:pDest-8; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_159690 Copy
Species: Homo sapiens
Genetic Insert: spacer of sgRNA targeting HOXD13 gene
Vector Backbone Description: Vector Backbone:pGL3-U6-sgRNA-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_159793 Copy
Species: Homo sapiens
Genetic Insert: spacer of pegRNA_HOXD13_CtoG targeting HOXD13 gene
Vector Backbone Description: Vector Backbone:pGL3-U6-sgRNA-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Other insert name: RT&PBS of pegRNA_HOXD13_CtoG targting HOXD13 gene
Proper citation: RRID:Addgene_159791 Copy
Species: Homo sapiens
Genetic Insert: spacer of sgRNA targeting VEGFA gene
Vector Backbone Description: Vector Backbone:pGL3-U6-sgRNA-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_159790 Copy
Species: Homo sapiens
Genetic Insert: MAP7 (only amino acids 1-316)(includes MTBD and P123 domains)
Vector Backbone Description: Vector Backbone:pOmnibac; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_159728 Copy
Species: Homo sapiens
Genetic Insert: KIF5B (full length, 1-963 amino acids), K963
Vector Backbone Description: Vector Backbone:pOmnibac; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_159727 Copy
Species: Homo sapiens
Genetic Insert: DMD Homology Arms with linker-mEGFP and Cas9-excisable selection cassette
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 in a two-step editing process to add a mEGFP tag to the C-terminus of human DMD in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. After initial HDR, stable expression of CAGGS-driven mCherry will indicate cells with integrated donor sequence. This selection cassette is designed to be removed with subsequent CRISPR/Cas9-mediated excision as previously described (https://doi.org/10.1101/342881). A linker of 3-21 amino acids will be produced after excision. To enrich for edited cells, see our protocol for fluorescence-assisted cell sorting and subcloning of hiPSCs (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ . Please visit https://www.biorxiv.org/content/early/2018/06/09/342881 for BioRxiv preprint
Proper citation: RRID:Addgene_159744 Copy
Species: Homo sapiens
Genetic Insert: TFAM Homology Arms with linker-mEGFP
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the C-terminus of human TFAM in WTC-11 human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: GGGS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
Proper citation: RRID:Addgene_159745 Copy
Species: Homo sapiens
Genetic Insert: ACE2
Vector Backbone Description: Vector Backbone:pFUSE; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
References:
Comments: Please visit https://doi.org/10.1101/2020.07.31.231746 for bioRxiv preprint.
Proper citation: RRID:Addgene_159762 Copy
Species: Homo sapiens
Genetic Insert: spacer of sgRNA targeting FANCF gene
Vector Backbone Description: Vector Backbone:pGL3-U6-sgRNA-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_159786 Copy
Species: Homo sapiens
Genetic Insert: spacer of pegRNA targeting KCNA1 gene
Vector Backbone Description: Vector Backbone:pGL3-U6-sgRNA-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Other insert name: RT&PBS of pegRNA targting KCNA1 gene
Proper citation: RRID:Addgene_159787 Copy
Species: Homo sapiens
Genetic Insert: spacer of sgRNA targeting EMX1gene
Vector Backbone Description: Vector Backbone:pGL3-U6-sgRNA-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_159784 Copy
Species: Homo sapiens
Genetic Insert: spacer of sgRNA targeting SRD5A1 gene
Vector Backbone Description: Vector Backbone:pGL3-U6-sgRNA-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_159780 Copy
Species: Homo sapiens
Genetic Insert: spacer of pegRNA targeting SRD5A3 gene
Vector Backbone Description: Vector Backbone:pGL3-U6-sgRNA-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Other insert name: RT&PBS of pegRNA targting SRD5A3 gene
Proper citation: RRID:Addgene_159781 Copy
Species: Homo sapiens
Genetic Insert: GSK3beta
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pCR3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_15994 Copy
Species: Homo sapiens
Genetic Insert: CHAMP1
Vector Backbone Description: Backbone Marker:Clontech (TaKaRa); Backbone Size:4731; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Addgene QC identified a partial deletion of the SV40 promoter compared to the hypothetical plasmid sequence. The depositing lab does not consider this to be of concern in regard to the function of this plasmid
Proper citation: RRID:Addgene_159892 Copy
Species: Homo sapiens
Genetic Insert: NUP188
Vector Backbone Description: Backbone Marker:Clontech (TaKaRa); Backbone Size:3965; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Addgene NGS had amino acids RHPS (at position 1051 to 1054) deleted and K206A on EGFP, but depositor confirms that these discrepancies do not have any functional significance on the plasmid.
Proper citation: RRID:Addgene_159893 Copy
Species: Homo sapiens
Genetic Insert: RPL10a
Vector Backbone Description: Backbone Size:6909; Vector Backbone:pSicoR-eIF1a-mCherry; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_159890 Copy
Species: Homo sapiens
Genetic Insert: Akt1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6600; Vector Backbone:pLNCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: The PH domain of AKT1 has been deleted.
Proper citation: RRID:Addgene_15989 Copy
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