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http://www.addgene.org/171693

Species: Mus musculus
Genetic Insert: ccdB-sgRNA scaffold-boxB-tBE-V5-mA3
Vector Backbone Description: Vector Backbone:pEF1a; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: The tBE system with gRNA could be constructed by linearing pU6-ccdB-boxB-tBE-V5-mA3 (backbone vector of tBE system) and inserting the gRNA sequences amplificating from pUC57-sgRNA-MS2-U6. The detailed protocol could be found in Supplementary Protocol in the publication.

Proper citation: RRID:Addgene_171693 Copy   

•   Source: Addgene

http://www.addgene.org/171791

Species: Mus musculus
Genetic Insert: ATP10B
Vector Backbone Description: Backbone Marker:Didier Nègre; Backbone Size:7445; Vector Backbone:SIV tranfserplasmide; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_171791 Copy   

•   Source: Addgene

http://www.addgene.org/171825

Species: Mus musculus
Genetic Insert: ATP10B
Vector Backbone Description: Backbone Marker:Didier Nègre; Backbone Size:7445; Vector Backbone:SIV tranfserplasmide; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_171825 Copy   

•   Source: Addgene

http://www.addgene.org/171771

Species: Mus musculus
Genetic Insert: ATP13A2
Vector Backbone Description: Backbone Marker:Didier Nègre; Backbone Size:7445; Vector Backbone:SIV tranfserplasmide; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_171771 Copy   

•   Source: Addgene

http://www.addgene.org/172006

Species: Mus musculus
Genetic Insert: Mouse PE sequence (mPE)
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5010; Vector Backbone:pGL3-Promoter; Vector Types:Mammalian Expression, Bacterial Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_172006 Copy   

•   Source: Addgene

http://www.addgene.org/171943

Species: Mus musculus
Genetic Insert: SNX9
Vector Backbone Description: Vector Backbone:pLenti6 V5-DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_171943 Copy   

•   Source: Addgene

http://www.addgene.org/16804

Species: Mus musculus
Genetic Insert: mFZ3-CS2+
Vector Backbone Description: Backbone Size:4095; Vector Backbone:CS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Insert: EcoRI to XbaI 2.3 kd clone mFZ3/pRK5 from Nathans lab. Contains full coding (with engineered optimal kozak sequence) sequence of mouse frizzled 3 (accession # U43205) Plasmid History: Constructed by: Rebecca Bates/Randall Moon. Notes for Use: Ref: Sheldahl et al., Current Biology, 9:695-698, 1999.

Proper citation: RRID:Addgene_16804 Copy   

•   Source: Addgene

http://www.addgene.org/16803

Species: Mus musculus
Genetic Insert: mFZ4-CS2+
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Insert: The clone mFZ4/pRK5 from Nathans lab (accession # U43317), was used as template to PCR amplify just the coding region 5' (methionine start) site is ClaI, and 3' stop site followed by XbaI. Constructed by: Rebecca Bates/Randall Moon. Ref: Sheldahl et al., Current Biology, 9:695-698, 1999. Addgene results indicate that the first 6aa are correct and there is a large gap from aa 7-143. This is not known to adversely affect function.

Proper citation: RRID:Addgene_16803 Copy   

•   Source: Addgene

http://www.addgene.org/16800

Species: Mus musculus
Genetic Insert: mFZ8-CS2+
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Insert: 2.2 Kb EcoR1 (5') - XbaI (3') insert of mFZ8/pRK5 (Nathans) with engineered optimal kozak sequence. Plasmid History: Constructed by: Rebecca Bates/Randall Moon. Ref: Sheldahl et al., Current Biology, 9:695-698, 1999.

Proper citation: RRID:Addgene_16800 Copy   

•   Source: Addgene

http://www.addgene.org/168280

Species: Mus musculus
Genetic Insert: Bin1
Vector Backbone Description: Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_168280 Copy   

•   Source: Addgene

http://www.addgene.org/168296

Species: Mus musculus
Genetic Insert: hU6-sgBmal1-1-hU6-sgBmal1-3
Vector Backbone Description: Backbone Size:5294; Vector Backbone:pAAV-hU6-gRNA-hSyn-mCherry-KASH; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments: When comparing to the depositor reference sequence, please note that the mutations K14E, N210D, and E249G in mCherry-KASH do not alter plasmid function.

Proper citation: RRID:Addgene_168296 Copy   

•   Source: Addgene

http://www.addgene.org/168281

Species: Mus musculus
Genetic Insert: hU6-sgPer1-3-hU6-sgPer2-1-hU6-sgPer2-2
Vector Backbone Description: Backbone Size:5294; Vector Backbone:pAAV-hU6-gRNA-hSyn-mCherry-KASH; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments: When comparing to the depositor reference sequence, please note that the mutations K14E, N210D, and E249G in mCherry-KASH do not alter plasmid function.

Proper citation: RRID:Addgene_168281 Copy   

•   Source: Addgene

http://www.addgene.org/168283

Species: Mus musculus
Genetic Insert: Bin1
Vector Backbone Description: Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_168283 Copy   

•   Source: Addgene

http://www.addgene.org/16823

Species: Mus musculus
Genetic Insert: mouse Chibby-pCS2+
Vector Backbone Description: Backbone Marker:RZPD; Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Description: The full coding sequence of mouse Chibby, ~0.4kb, was amplified by PCR omitting the UTRs, and subcloned into the BamH1 and Xho1 sites of pCS2+. Reference: K. Takemaru et al., Nature 422: 905 (2003)

Proper citation: RRID:Addgene_16823 Copy   

•   Source: Addgene

http://www.addgene.org/16850

Species: Mus musculus
Genetic Insert: Wnt-1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2P+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Description: Full coding sequence of mouse Wnt-1, untagged. ~1.5kb insert isolated from Ajamete Kaykas's 2294 mWnt1 plasmid, Not1filled/Spe1 fragment cloned into pCS2P+ Stu1/Xba1. Notes for Use: For in vitro transcription linearize with Asp718 and transcribe with SP6. Reference: Clone made by Gilbert Weidinger, Moon lab.

Proper citation: RRID:Addgene_16850 Copy   

•   Source: Addgene

http://www.addgene.org/16860

Species: Mus musculus
Genetic Insert: dnWnt-1
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Plasmid History: Constructed by Jeff Brown on date of 8/8/95. Transcription: For sense, linearize with Asp718 (BMB) and transcribe with SP6. PCR amplification from clone 286 (McMahon and Moon). Also referred to as int-1 spnfc or int-1 spnfcD. The Wnt-1 gene is truncated after the SPNFC peptide sequence. Ref: Hoppler et al., Genes & Development, 10:2805-2817, 1996.

Proper citation: RRID:Addgene_16860 Copy   

•   Source: Addgene

http://www.addgene.org/168510

Species: Mus musculus
Genetic Insert: Synaptophysin-pHluorin
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_168510 Copy   

•   Source: Addgene

http://www.addgene.org/16859

Species: Mus musculus
Genetic Insert: dnWnt-1 myc CS2+
Vector Backbone Description: Backbone Size:5010; Vector Backbone:pCS2P+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Transcription: For sense, linearize with Asp718 (BMB) and transcribe with. SP6. PCR amplified from myc tagged mouse Wnt-1 (McMahon and Moon). Also. referred to as int-1 spnfc or int-1 spnfcD in notebook #3. The Wnt-1 gene. is truncated acter the SPNFC peptide sequence. Notes for Use: Constructed by J. Brown.

Proper citation: RRID:Addgene_16859 Copy   

•   Source: Addgene

http://www.addgene.org/16854

Species: Mus musculus
Genetic Insert: Wnt1
Vector Backbone Description: Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression, Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Description: Full coding sequence of mouse Wnt-1 (int-1 in early literature), with one internal c-myc epitope, in the Stratagene vector, pcDNA3. Protein can be immunoprecipitated, recognized in whole mount immunostaining, and detected by western blot, using the anti-c-myc antibody 9E-10. Species of Sequence Origin: mouse. Notes for Use: Uncharacterized. Reference: The insert was published as p284 in AP McMahon and RT Moon, Cell 58: 1075-1084 (1989). This unpublished version was generated by Julia Yang-Snyder.

Proper citation: RRID:Addgene_16854 Copy   

•   Source: Addgene

http://www.addgene.org/16852

Species: Mus musculus
Genetic Insert: antisense Wnt-1
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCD-betaG-PS; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
References:
Comments: Description: Antisense orientation of full coding sequence of mouse Wnt-1 (int-1 in early literature), with one internal c-myc epitope, cloned into the SV40 expression vector pCD-PS, but providing Xenopus beta globin 5' and 3' UTRs. Species of Sequence Origin: mouse. Notes for Use: Uncharacterized. Reference: The insert was published as p284 in AP McMahon and RT Moon, Cell 58: 1075-1084 (1989). This unpublished construct was generated by Julia Yang-Snyder.

Proper citation: RRID:Addgene_16852 Copy   

•   Source: Addgene