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Species: Lachnospiraceae bacterium
Genetic Insert: dCas12a
Vector Backbone Description: Vector Backbone:LentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36162812
Comments: Stbl3 is also a suitable growth strain.
Proper citation: RRID:Addgene_188513 Copy
Species: Homo sapiens
Genetic Insert: epoxide hydrolase 1
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pcmv entry; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34342583
Proper citation: RRID:Addgene_169172 Copy
Species: Homo sapiens
Genetic Insert: epoxide hydrolase 1
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pcmv entry; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34342583
Proper citation: RRID:Addgene_169173 Copy
Species: Homo sapiens
Genetic Insert: OPTN
Vector Backbone Description: Backbone Marker:James Hurley Lab; Backbone Size:4905; Vector Backbone:pGST2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37207627
Proper citation: RRID:Addgene_187827 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Backbone Marker:Víctor de Lorenzo; Backbone Size:3730; Vector Backbone:pSEVA221; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36511859
Proper citation: RRID:Addgene_188965 Copy
Species: Homo sapiens
Genetic Insert: AKT1
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_189890 Copy
Species: Homo sapiens
Genetic Insert: KIF1C
Vector Backbone Description: Vector Backbone:pFastbacM13; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35961316
Proper citation: RRID:Addgene_185979 Copy
Species: Homo sapiens
Genetic Insert: ANKRD1 KO sgRNA Plasmid
Vector Backbone Description: Vector Backbone:pL-CRISPR.EFS.GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35639929
Comments: sgRNA sequence: GCTATATAAGCTGACCGGTG. The corresponding genome sequence is: GCTATATAAGCTGACCGGTG.
Please visit https://www.biorxiv.org/content/10.1101/2020.12.18.423517v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_186669 Copy
Vector Backbone Description: Backbone Marker:John Dueber; Vector Backbone:pYTK090; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37930278
Comments: RFP expression in E. coli. Plasmid yields may drop after multiple passages or delayed miniprepping after the culture has saturated. We suggest streaking onto solid media to pick fresh colonies every time.
Proper citation: RRID:Addgene_180729 Copy
Genetic Insert: KRAB-dCas9-MeCP2
Vector Backbone Description: Backbone Size:8800; Vector Backbone:pFUGW; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37141313
Comments: Please visit https://doi.org/10.1101/2021.04.07.438882 for bioRxiv preprint.
Proper citation: RRID:Addgene_170068 Copy
Species: Synthetic
Genetic Insert: 50%GC-40bp-hairpin-binding-site-reversed
Vector Backbone Description: Backbone Size:3956; Vector Backbone:pCR-XL-2-TOPO; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36063324
Comments: Because this sequence contains an intentional hairpin the plasmid should be cut with BstXI when sequencing this region.
Proper citation: RRID:Addgene_169198 Copy
Species: Synthetic
Genetic Insert: 66%GC-20bp-hairpin
Vector Backbone Description: Backbone Size:3519; Vector Backbone:pCR-XL-TOPO; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36063324
Comments: Because this sequence contains an intentional hairpin the plasmid should be cut with BstXI when sequencing this region.
Proper citation: RRID:Addgene_169199 Copy
Species: Synthetic
Genetic Insert: Luc-E2A-mCherry-T2A-PuroR
Vector Backbone Description: Backbone Marker:I. Verma, Salk; Backbone Size:9221; Vector Backbone:p.FUW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19723471
Proper citation: RRID:Addgene_183685 Copy
Species: Synthetic
Genetic Insert: yETSapphire
Vector Backbone Description: Vector Backbone:pM; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Please note: Plasmid contains a M233I mutation in yETSapphire. This mutation does not affect plasmid function.
Proper citation: RRID:Addgene_176569 Copy
Species: Synthetic
Genetic Insert: 50%GC-20bp-hairpin
Vector Backbone Description: Backbone Size:3519; Vector Backbone:pCR-XL-TOPO; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36063324
Comments: Because this sequence contains an intentional hairpin the plasmid should be cut with BstXI when sequencing this region.
Proper citation: RRID:Addgene_169196 Copy
Species: Synthetic
Genetic Insert: 50%GC-40bp-hairpin
Vector Backbone Description: Backbone Size:3519; Vector Backbone:pCR-XL-TOPO; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36063324
Comments: Because this sequence contains an intentional hairpin the plasmid should be cut with BstXI when sequencing this region.
Proper citation: RRID:Addgene_169197 Copy
Species: Synthetic
Genetic Insert: TagRFP657
Vector Backbone Description: Vector Backbone:pU; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_176562 Copy
Species: Lanchea thermotolerans
Genetic Insert: YLL058W-lt
Vector Backbone Description: Vector Backbone:pU; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_176564 Copy
Species: Synthetic
Genetic Insert: yETSapphire
Vector Backbone Description: Vector Backbone:pH; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Please note: Plasmid contains a M233I mutation in yETSapphire. This mutation does not affect plasmid function.
Proper citation: RRID:Addgene_176566 Copy
Species: Zygosaccharomyces rouxii
Genetic Insert: YLL058W-zr
Vector Backbone Description: Vector Backbone:pU; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_176565 Copy
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