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Species: Homo sapiens
Genetic Insert: CasRx
Vector Backbone Description: Backbone Size:6487; Vector Backbone:Addgene #154398; Vector Types:Mammalian Expression, Unspecified, Piggybac transposon; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_155183 Copy
Species: Homo sapiens
Genetic Insert: PA1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_15559 Copy
Species: Homo sapiens
Genetic Insert: DPY30
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pWZL neo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: FLAG-tagged hDPY-30 was cloned by PCR from HeLaS cDNA using primers 5'-C AGT AGG ATC CTG GCC ACC ATG GAC TAC AAG GAC GAC GAT GAC AAG ATG GAG CCA GAG CAG ATG CTG G-3' and 5'-TCG CTG CTC GAG TCA GTT TCG ATC TTC AAA CTG TGC-3'.
Proper citation: RRID:Addgene_15555 Copy
Species: Homo sapiens
Genetic Insert: RbBP5
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4972; Vector Backbone:pGEX-5X1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_15551 Copy
Species: Homo sapiens
Genetic Insert: RbBP5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_15550 Copy
Species: Homo sapiens
Genetic Insert: YTHDF3(1-385)
Vector Backbone Description: Backbone Marker:New England Biolabs; Vector Backbone:pSNAPf; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_155353 Copy
Species: Homo sapiens
Genetic Insert: YTHDF1(264-559)
Vector Backbone Description: Vector Backbone:pFUGW-CRY2olig-BFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_155354 Copy
Species: Homo sapiens
Genetic Insert: YTHDF1 (1-359)
Vector Backbone Description: Backbone Marker:New England Biolabs; Vector Backbone:pSNAPf; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_155351 Copy
Species: Homo sapiens
Genetic Insert: htt 25Q Delta Pro
Vector Backbone Description: Backbone Size:5800; Vector Backbone:p416 GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: Figure 2, III in article.
Lindquist lab number: 7536
Proper citation: RRID:Addgene_15568 Copy
Species: Homo sapiens
Genetic Insert: FKBP12(V36)-p30Caspase9
Vector Backbone Description: Backbone Size:5300; Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: Based on L. Fan et al (99) Human Gene Therapy
Proper citation: RRID:Addgene_15567 Copy
Species: Homo sapiens
Genetic Insert: MRE11 shRNA
Vector Backbone Description: Backbone Size:3176; Vector Backbone:pSUPER; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
References:
Comments: Sense oligo sequence used for cloning: MRE11, 5'-GATCCCCGGC ACTGAGAAAC ATGCAATTCA AGAGATTGCA TGTTTCTCAG TGCCTTTTTG GAAA-3'.
Proper citation: RRID:Addgene_15566 Copy
Species: Homo sapiens
Genetic Insert: FOXC2
Vector Backbone Description: Backbone Marker:Available at Addgene (#1764); Backbone Size:5169; Vector Backbone:pBabe puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_15535 Copy
Species: Homo sapiens
Genetic Insert: Ash2L
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4972; Vector Backbone:pGEX-5X1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Full-length ASH2L cDNA was amplified by reverse transcription-PCR from HeLaS cells using primers 5'-TAT AGG ATC CTG GTG ATG GCG GCG GCA GGA G-3' and 5'-CAG CGA CTC GAG TCA GGG TTC CCA TGG GGG AC-3'.
Proper citation: RRID:Addgene_15549 Copy
Species: Homo sapiens
Genetic Insert: Ash2L
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Full-length ASH2L cDNA was amplified by reverse transcription-PCR from HeLaS cells using primers 5'-TAT AGG ATC CTG GTG ATG GCG GCG GCA GGA G-3' and 5'-CAG CGA CTC GAG TCA GGG TTC CCA TGG GGG AC-3'
Please note that mutation R622H in ASH2L was found during Addgene's quality control. The plasmid should function as described.
Proper citation: RRID:Addgene_15548 Copy
Species: Homo sapiens
Genetic Insert: htt 103Q Delta Pro
Vector Backbone Description: Backbone Size:0; Vector Backbone:p303 Met; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: Figure 1C in article.
Lindquist lab number: 8056
Due to slippage when polymerases go through them, the Q stretches often get shorter over time due to mistakes while they are replicated in the cells. The shortening should not have much of an effect until the Q stretches get into the 80s.
Proper citation: RRID:Addgene_15591 Copy
Species: Homo sapiens
Genetic Insert: htt 103Q Delta Pro
Vector Backbone Description: Backbone Size:0; Vector Backbone:p303 Gal; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: Figure 2, VI in article.
Lindquist lab number: 7704
Proper citation: RRID:Addgene_15579 Copy
Species: Homo sapiens
Genetic Insert: htt 25Q Delta Pro
Vector Backbone Description: Backbone Size:0; Vector Backbone:p303 Gal; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: Figure 2, VI in article.
Lindquist lab number: 7703
Proper citation: RRID:Addgene_15578 Copy
Species: Homo sapiens
Genetic Insert: htt25Q + Pro
Vector Backbone Description: Backbone Size:0; Vector Backbone:p303 Gal; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: Figure 1, I in article.
Lindquist lab number: 7570
Proper citation: RRID:Addgene_15574 Copy
Species: Homo sapiens
Genetic Insert: htt 25Q + Pro
Vector Backbone Description: Backbone Size:0; Vector Backbone:p303 Gal; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: Figure 2, V in article.
Lindquist lab number: 7699
Due to slippage when polymerases go through them, the Q stretches often get shorter over time due to mistakes while they are replicated in the cells. The shortening should not have much of an effect until the Q stretches get into the 80s.
Proper citation: RRID:Addgene_15570 Copy
Species: Homo sapiens
Genetic Insert: htt 103Q Delta Pro
Vector Backbone Description: Backbone Size:0; Vector Backbone:p303 Gal; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: Figure 1C in article.
Lindquist lab number: 7720
Due to slippage when polymerases go through them, the Q stretches often get shorter over time due to mistakes while they are replicated in the cells. The shortening should not have much of an effect until the Q stretches get into the 80s.
Proper citation: RRID:Addgene_15587 Copy
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