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Species: Homo sapiens
Genetic Insert: F-CDK8L
Vector Backbone Description: Backbone Marker:Addgene plasmid 15439; Backbone Size:5600; Vector Backbone:pcDNA3.1 N-FLAG/Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15680972
Proper citation: RRID:Addgene_24762 Copy
Species: Homo sapiens
Genetic Insert: Pirh2
Vector Backbone Description: Backbone Size:0; Vector Backbone:pGEX-2TKm; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19043414
Comments: E. coli strain for expression: BL21DE3
Expression conditions: Pirh2 proteins need Zn2+ to fold properly, so 50 uM of ZnCl2 is added to the growth media and purification buffers.
Induction conditions: 0.5mM-1.0mM IPTG at RT for 3.5-4 hours.
For pGEX-2TKm vector, the kinase site RRASV is removed and NdeI site is inserted at 5' of BamHI.
Proper citation: RRID:Addgene_24882 Copy
Species: Homo sapiens
Genetic Insert: TAZ
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCMV5-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18568018
Proper citation: RRID:Addgene_24814 Copy
Species: Homo sapiens
Genetic Insert: hRab8A
Vector Backbone Description: Backbone Size:6100; Vector Backbone:pDEST53; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17574030
Proper citation: RRID:Addgene_24898 Copy
Species: Homo sapiens
Genetic Insert: hRab8A[T22N]
Vector Backbone Description: Backbone Size:6100; Vector Backbone:pDEST53; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17574030
Proper citation: RRID:Addgene_24899 Copy
Species: Homo sapiens
Genetic Insert: p35
Vector Backbone Description: Backbone Size:4969; Vector Backbone:pGEX6P-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11813240
Proper citation: RRID:Addgene_24895 Copy
Species: Homo sapiens
Genetic Insert: shRNA-GFP
Vector Backbone Description: Backbone Marker:Halo-Bio RNAi Therapeutics; Backbone Size:3176; Vector Backbone:pSUPER; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16611886
Comments: shRNA oligo sequence is - 5′-GCAAGCTGACCCTGAAGTTC
Proper citation: RRID:Addgene_24747 Copy
Species: Homo sapiens
Genetic Insert: p53
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11524676
Comments: E.Coli strain for epression: BL21DE3-pLysS
p53 proteins are expressed better and more stable at low temperature
Proper citation: RRID:Addgene_24868 Copy
Species: Homo sapiens
Genetic Insert: (sex determining region Y)-box 10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2545; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19422606
Proper citation: RRID:Addgene_24749 Copy
Species: Homo sapiens
Genetic Insert: USP5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5300; Vector Backbone:pcDNA-4C; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19553310
Proper citation: RRID:Addgene_24744 Copy
Species: Homo sapiens
Genetic Insert: p53
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11524676
Comments: E.Coli strain for epression: BL21DE3-pLysS
p53 proteins are expressed better and more stable at low temperature
Proper citation: RRID:Addgene_24864 Copy
Species: Homo sapiens
Genetic Insert: p53
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11524676
Comments: E.Coli strain for epression: BL21DE3
Induction condition: 0.3 mM IPTG at RT for 3 hours or 15 degrees Celsius, overnight.
NdeI site is inserted at 5'-end of BamHI site on pGEX-2TK vector.
Proper citation: RRID:Addgene_24860 Copy
Species: Homo sapiens
Genetic Insert: Sirt3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5075; Vector Backbone:pcDNA4/myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18794531
Comments: Catalytically inactive mutant
Proper citation: RRID:Addgene_24917 Copy
Species: Homo sapiens
Genetic Insert: Rabin8
Vector Backbone Description: Backbone Size:2500; Vector Backbone:pDONR; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17574030
Proper citation: RRID:Addgene_24910 Copy
Species: Homo sapiens
Genetic Insert: p53
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11524676
Comments: E.Coli strain for epression: BL21DE3
Induction condition: 0.3 mM IPTG at RT for 3 hours or 15 degrees Celsius, overnight.
NdeI site is inserted at 5'-end of BamHI site on pGEX-2TK vector.
Proper citation: RRID:Addgene_24870 Copy
Species: Homo sapiens
Genetic Insert: (sex determining region Y)-box 10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2545; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19422606
Proper citation: RRID:Addgene_24738 Copy
Species: Homo sapiens
Genetic Insert: p53
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11524676
Comments: E.Coli strain for epression: BL21DE3-pLysS
p53 proteins are expressed better and more stable at low temperature
Proper citation: RRID:Addgene_24859 Copy
Species: Homo sapiens
Genetic Insert: (sex determining region Y)-box 10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2545; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19422606
Proper citation: RRID:Addgene_24735 Copy
Species: Homo sapiens
Genetic Insert: BRaf-ER
Vector Backbone Description: Backbone Size:11100; Vector Backbone:LZRS no LacZ; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11896581
Proper citation: RRID:Addgene_24949 Copy
Species: Homo sapiens
Genetic Insert: GADD45
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5300; Vector Backbone:pcDNA3-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10973963
Comments: Plasmid size: 5867 bp
The HA sequence is between XbaI and ApaI restriction enzyme sites.
Proper citation: RRID:Addgene_24929 Copy
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