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Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 Spike E484Q
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.medrxiv.org/content/10.1101/2024.03.05.24303815v1 for medRxiv preprint.
Proper citation: RRID:Addgene_212423 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Nsp15-H234A
Vector Backbone Description: Backbone Marker:purchased from GenScript; Backbone Size:5644; Vector Backbone:pET-11b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Protein sequence is MHHHHHHSSGVDLGTENLYFQSNAM + Nsp15-H234A (amino acid residues 6453-6798 of YP_009724389, with H234A mutation).
Confers ampicillin-resistance.
Backbone vector is pET-11b from GenScript.
Mutation design by Nirupa Nagaratnam.
Proper citation: RRID:Addgene_219529 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Nsp13_SARS2
Vector Backbone Description: Vector Backbone:pFBD-BirA; Vector Types:Baculovirus Expresssion; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_234375 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Nsp7_SARS2
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_234405 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Nsp3_SARS2
Vector Backbone Description: Vector Backbone:pNIC-Bio3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_234389 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Nsp14_SARS2
Vector Backbone Description: Vector Backbone:p28BIOH-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_234376 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 S HexaPro
Vector Backbone Description: Vector Backbone:AAVS1_Puro_PGK1_3xFLAG_Twin_Strep (Addgene plasmid # 68375); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: The promoter and insert elements were derived from SARS-CoV-2 S HexaPro, a gift from Jason McLellan (Addgene plasmid # 154754).
Proper citation: RRID:Addgene_164077 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Spike (S-RRAR-D614G variant)
Vector Backbone Description: Backbone Size:4746; Vector Backbone:pαH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164570 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Spike (S-GSAS-D614G variant)
Vector Backbone Description: Backbone Size:4746; Vector Backbone:pαH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164566 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Spike - Furin site changed for HRV3C site
Vector Backbone Description: Backbone Size:4746; Vector Backbone:pαH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164567 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Spike (S-RRAR variant)
Vector Backbone Description: Backbone Size:4746; Vector Backbone:pαH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164569 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: nsp12
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3829; Vector Backbone:pRSFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_165451 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 S B.1.351
Vector Backbone Description: Vector Backbone:pcDNA5/FRT/TO/intron; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.30.437622v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_172736 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 S HexaPro-D614G
Vector Backbone Description: Vector Backbone:YTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.30.437622v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_172733 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 S B.1.427/B.1.429
Vector Backbone Description: Vector Backbone:pcDNA5/FRT/TO/intron; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.30.437622v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_172738 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 S HexaPro-D614G
Vector Backbone Description: Vector Backbone:pcDNA5/FRT/TO/intron; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.30.437622v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_172725 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 S HexaPro-D614G
Vector Backbone Description: Vector Backbone:pcDNA5/FRT/TO/intron; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.30.437622v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_172723 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Spike (S-GSAS-B.1.351 variant version 3)
Vector Backbone Description: Backbone Size:4746; Vector Backbone:pαH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_173787 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Spike (S-GSAS-B.1.351 variant version 4)
Vector Backbone Description: Backbone Size:4746; Vector Backbone:pαH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_173788 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Spike S-GSAS- P-1-like variant (K417N)
Vector Backbone Description: Backbone Size:4746; Vector Backbone:pαH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_173789 Copy
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