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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
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|---|---|---|---|---|---|---|---|---|---|---|
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pLexm-gfp-Pept2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_167988 | PepT2 | Rattus norvegicus | Ampicillin | PMID:34433568 | Backbone Size:5400; Vector Backbone:pLexM-GFPHis8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-12 02:35:34 | 1 | ||
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AAV2-hSyn-G-Flamp1 Resource Report Resource Website |
RRID:Addgene_188569 | G-Flamp1 | Synthetic | Ampicillin | PMID:36097007 | Please visit https://www.biorxiv.org/content/10.1101/2022.02.27.482140v1 for bioRxiv preprint. | Backbone Size:4495; Vector Backbone:pAAV-hSyn; Vector Types:AAV; Bacterial Resistance:Ampicillin | 2026-09-12 02:35:35 | 0 | |
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pDEST26-his-hSFRP1 Resource Report Resource Website |
RRID:Addgene_189539 | secreted frizzled related protein 1 | Homo sapiens | Ampicillin | Vector Backbone:pDEST26; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-12 02:35:35 | 0 | |||
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pcDNA3.2-V5-DEST-hSFRP1-GST Resource Report Resource Website |
RRID:Addgene_189543 | secreted frizzled related protein 1 | Homo sapiens | Ampicillin | Backbone Marker:Customized backbone from Fu lab ; Vector Backbone:pcDNA3.2-V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-12 02:35:35 | 0 | |||
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pJEC726 Resource Report Resource Website |
RRID:Addgene_190814 | dCas9 | Synthetic | Apramycin | PMID:35801861 | Backbone Marker:Addgene; Backbone Size:6573; Vector Backbone:pCRISPomyces-2; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin | S. pyogenes dCas9 codon optimized for Streptomyces expression. The D10A and H840A mutations were introduced to convert Cas9 to dCas9. | 2026-09-12 02:35:34 | 0 | |
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pJEC819 Resource Report Resource Website |
RRID:Addgene_190815 | dCas9-XTEN-rpoA | Synthetic | Apramycin | PMID:35801861 | Backbone Size:6578; Vector Backbone:pCRISPomyces-2; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin | 2026-09-12 02:35:34 | 0 | ||
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pET-Duet1_6xHis-TEV-eGFP-P62 Resource Report Resource Website |
RRID:Addgene_190929 | P62 | Homo sapiens | Ampicillin | PMID:34471133 | https://hub.asap.science/shared-research/1823e9d4-44a1-4cc7-9cc7-f9557ff1f152 | Backbone Marker:EMD Biosciences; Backbone Size:5420; Vector Backbone:pET-Duet1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-09-12 02:35:34 | 0 | |
|
pEYFP-C1-NuMA Resource Report Resource Website 1+ mentions |
RRID:Addgene_28238 | Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1) | Homo sapiens | Kanamycin | NuMA insert contains E873K and L1575F mutations compared to best match reference sequence (NP_001273490.1). | Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-09-12 02:35:34 | 2 | ||
|
pET21a-cys-His6-OtUBD Resource Report Resource Website 1+ mentions |
RRID:Addgene_190091 | OtUBD | Orientia Tsutsugamushi | Ampicillin | PMID:35771886 | Backbone Size:5443; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-09-12 02:35:34 | 2 | ||
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ReactionC_pBIG1a_ATG7_ATG10 Resource Report Resource Website |
RRID:Addgene_190860 | ATG7 | Homo sapiens | Ampicillin | PMID:32437499 | https://hub.asap.science/shared-research/3633829c-35dd-43f5-b9ce-bcb709126a0f | Backbone Marker:Jan-Michael Peters; Backbone Size:5915; Vector Backbone:pBIG1a; Vector Types:Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin | 2026-09-12 02:35:34 | 0 | |
|
p663-UBC-miniTurbo-V5-NEK5_IDG-K Resource Report Resource Website |
RRID:Addgene_189876 | NEK5 | Homo sapiens | Ampicillin | These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. | Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin | 2026-09-12 02:35:35 | 0 | ||
|
dTAG-IRF8 Resource Report Resource Website |
RRID:Addgene_190620 | IRF8 | Homo sapiens | Ampicillin | PMID:34358447 | Vector Backbone:LentiV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | synonymous mutation for sgRNA resistance for sgIRF8_1 | 2026-09-12 02:35:34 | 0 | |
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pGEX-4T1_ATG4B_BamHI_NotI Resource Report Resource Website 1+ mentions |
RRID:Addgene_190862 | ATG4B | Homo sapiens | Ampicillin | PMID:32437499 | https://hub.asap.science/shared-research/c0b4a3f9-816a-485f-951c-e1435f469be2 | Backbone Marker:Amersham; Backbone Size:4969; Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-09-12 02:35:34 | 1 | |
|
p667-UBC-NUAK2-V5-miniTurbo_IDG-K Resource Report Resource Website |
RRID:Addgene_189910 | NUAK2 | Homo sapiens | Ampicillin | These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. | Backbone Marker:PEL; Backbone Size:9014; Vector Backbone:pDest667; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin | 2026-09-12 02:35:35 | 0 | ||
|
p663-UBC-miniTurbo-V5-STK17B_IDG-K Resource Report Resource Website |
RRID:Addgene_189878 | STK17B | Homo sapiens | Ampicillin | These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. | Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin | 2026-09-12 02:35:34 | 0 | ||
|
pLIB_BSSHI_6xHisTEV-WIPI2d Resource Report Resource Website |
RRID:Addgene_190863 | WIPI2 | Homo sapiens | Ampicillin | https://hub.asap.science/shared-research/5cc86719-88d5-48ac-b648-c96aecd5afb6 | Backbone Marker:Jan-Michael Peters; Backbone Size:4970; Vector Backbone:pLIB; Vector Types:Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin | SNP C to G at position 4562 (Val) | 2026-09-12 02:35:34 | 0 | |
|
p667-UBC-SCYL1-V5-miniTurbo_IDG-K Resource Report Resource Website |
RRID:Addgene_189911 | SCYL1 | Homo sapiens | Ampicillin | These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. | Backbone Marker:PEL; Backbone Size:9014; Vector Backbone:pDest667; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin | 2026-09-12 02:35:35 | 0 | ||
|
p667-UBC-SCYL2-V5-miniTurbo_IDG-K Resource Report Resource Website |
RRID:Addgene_189912 | SCYL2 | Homo sapiens | Ampicillin | These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. | Backbone Marker:PEL; Backbone Size:9014; Vector Backbone:pDest667; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin | 2026-09-12 02:35:34 | 0 | ||
|
pXJ40-Flag-Ferritin-iBox-PAK4cat Resource Report Resource Website 1+ mentions |
RRID:Addgene_190180 | Ferritin-iBox-PAK4cat | Homo sapiens | Ampicillin | PMID:31552740 | Vector Backbone:pXJ40; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-12 02:35:34 | 1 | ||
|
pET-Duet1-6xHis-TEV-LC3BGlydelta5C Resource Report Resource Website 1+ mentions |
RRID:Addgene_190237 | MAP1LC3B | Homo sapiens | Ampicillin | PMID:32437499 | https://hub.asap.science/shared-research/1e41d81c-1a19-464c-bdec-522ee6220c8e | Backbone Marker:EMD Biosciences; Backbone Size:5420; Vector Backbone:pET Duet1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-09-12 02:35:34 | 1 |
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