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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pLexm-gfp-Pept2
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_167988 PepT2 Rattus norvegicus Ampicillin PMID:34433568 Backbone Size:5400; Vector Backbone:pLexM-GFPHis8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-12 02:35:34 1
AAV2-hSyn-G-Flamp1
 
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RRID:Addgene_188569 G-Flamp1 Synthetic Ampicillin PMID:36097007 Please visit https://www.biorxiv.org/content/10.1101/2022.02.27.482140v1 for bioRxiv preprint. Backbone Size:4495; Vector Backbone:pAAV-hSyn; Vector Types:AAV; Bacterial Resistance:Ampicillin 2026-09-12 02:35:35 0
pDEST26-his-hSFRP1
 
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RRID:Addgene_189539 secreted frizzled related protein 1 Homo sapiens Ampicillin Vector Backbone:pDEST26; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-12 02:35:35 0
pcDNA3.2-V5-DEST-hSFRP1-GST
 
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RRID:Addgene_189543 secreted frizzled related protein 1 Homo sapiens Ampicillin Backbone Marker:Customized backbone from Fu lab ; Vector Backbone:pcDNA3.2-V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-12 02:35:35 0
pJEC726
 
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RRID:Addgene_190814 dCas9 Synthetic Apramycin PMID:35801861 Backbone Marker:Addgene; Backbone Size:6573; Vector Backbone:pCRISPomyces-2; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin S. pyogenes dCas9 codon optimized for Streptomyces expression. The D10A and H840A mutations were introduced to convert Cas9 to dCas9. 2026-09-12 02:35:34 0
pJEC819
 
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RRID:Addgene_190815 dCas9-XTEN-rpoA Synthetic Apramycin PMID:35801861 Backbone Size:6578; Vector Backbone:pCRISPomyces-2; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin 2026-09-12 02:35:34 0
pET-Duet1_6xHis-TEV-eGFP-P62
 
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RRID:Addgene_190929 P62 Homo sapiens Ampicillin PMID:34471133 https://hub.asap.science/shared-research/1823e9d4-44a1-4cc7-9cc7-f9557ff1f152 Backbone Marker:EMD Biosciences; Backbone Size:5420; Vector Backbone:pET-Duet1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-09-12 02:35:34 0
pEYFP-C1-NuMA
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_28238 Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1) Homo sapiens Kanamycin NuMA insert contains E873K and L1575F mutations compared to best match reference sequence (NP_001273490.1). Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-09-12 02:35:34 2
pET21a-cys-His6-OtUBD
 
Resource Report
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1+ mentions
RRID:Addgene_190091 OtUBD Orientia Tsutsugamushi Ampicillin PMID:35771886 Backbone Size:5443; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-09-12 02:35:34 2
ReactionC_pBIG1a_ATG7_ATG10
 
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RRID:Addgene_190860 ATG7 Homo sapiens Ampicillin PMID:32437499 https://hub.asap.science/shared-research/3633829c-35dd-43f5-b9ce-bcb709126a0f Backbone Marker:Jan-Michael Peters; Backbone Size:5915; Vector Backbone:pBIG1a; Vector Types:Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin 2026-09-12 02:35:34 0
p663-UBC-miniTurbo-V5-NEK5_IDG-K
 
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RRID:Addgene_189876 NEK5 Homo sapiens Ampicillin These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin 2026-09-12 02:35:35 0
dTAG-IRF8
 
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RRID:Addgene_190620 IRF8 Homo sapiens Ampicillin PMID:34358447 Vector Backbone:LentiV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin synonymous mutation for sgRNA resistance for sgIRF8_1 2026-09-12 02:35:34 0
pGEX-4T1_ATG4B_BamHI_NotI
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_190862 ATG4B Homo sapiens Ampicillin PMID:32437499 https://hub.asap.science/shared-research/c0b4a3f9-816a-485f-951c-e1435f469be2 Backbone Marker:Amersham; Backbone Size:4969; Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-09-12 02:35:34 1
p667-UBC-NUAK2-V5-miniTurbo_IDG-K
 
Resource Report
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RRID:Addgene_189910 NUAK2 Homo sapiens Ampicillin These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. Backbone Marker:PEL; Backbone Size:9014; Vector Backbone:pDest667; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin 2026-09-12 02:35:35 0
p663-UBC-miniTurbo-V5-STK17B_IDG-K
 
Resource Report
Resource Website
RRID:Addgene_189878 STK17B Homo sapiens Ampicillin These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin 2026-09-12 02:35:34 0
pLIB_BSSHI_6xHisTEV-WIPI2d
 
Resource Report
Resource Website
RRID:Addgene_190863 WIPI2 Homo sapiens Ampicillin https://hub.asap.science/shared-research/5cc86719-88d5-48ac-b648-c96aecd5afb6 Backbone Marker:Jan-Michael Peters; Backbone Size:4970; Vector Backbone:pLIB; Vector Types:Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin SNP C to G at position 4562 (Val) 2026-09-12 02:35:34 0
p667-UBC-SCYL1-V5-miniTurbo_IDG-K
 
Resource Report
Resource Website
RRID:Addgene_189911 SCYL1 Homo sapiens Ampicillin These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. Backbone Marker:PEL; Backbone Size:9014; Vector Backbone:pDest667; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin 2026-09-12 02:35:35 0
p667-UBC-SCYL2-V5-miniTurbo_IDG-K
 
Resource Report
Resource Website
RRID:Addgene_189912 SCYL2 Homo sapiens Ampicillin These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. Backbone Marker:PEL; Backbone Size:9014; Vector Backbone:pDest667; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin 2026-09-12 02:35:34 0
pXJ40-Flag-Ferritin-iBox-PAK4cat
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_190180 Ferritin-iBox-PAK4cat Homo sapiens Ampicillin PMID:31552740 Vector Backbone:pXJ40; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-12 02:35:34 1
pET-Duet1-6xHis-TEV-LC3BGlydelta5C
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_190237 MAP1LC3B Homo sapiens Ampicillin PMID:32437499 https://hub.asap.science/shared-research/1e41d81c-1a19-464c-bdec-522ee6220c8e Backbone Marker:EMD Biosciences; Backbone Size:5420; Vector Backbone:pET Duet1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-09-12 02:35:34 1

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