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Species: Mus musculus
Genetic Insert: Olfr2 gRNA
Vector Backbone Description: Backbone Size:7099; Vector Backbone:pSIN; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_175935 Copy
Species: Mus musculus
Genetic Insert: Gapdh gRNA
Vector Backbone Description: Backbone Size:7097; Vector Backbone:pSIN; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_175938 Copy
Species: Mus musculus
Genetic Insert: rhotekin
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_176100 Copy
Species: Mus musculus
Genetic Insert: phosphatidylinositol 3-kinase
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://doi.org/10.1101/2022.10.17.512253 for bioRxiv preprint.
Proper citation: RRID:Addgene_176102 Copy
Species: Mus musculus
Genetic Insert: rhotekin
Vector Backbone Description: Vector Backbone:pEntr1A; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_176137 Copy
Species: Mus musculus
Genetic Insert: HES1
Vector Backbone Description: Backbone Marker:Linzhao Cheng Lab; Backbone Size:8200; Vector Backbone:EF.v-CMV.GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: The CMV promoter was inserted immediately upstream of GFP in the EF.GFP LV to make the intermediate LV, EF.V-CMV.GFP. mHES1 was cloned downstream of the EF-1a promoter.
Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes.
Proper citation: RRID:Addgene_17622 Copy
Species: Mus musculus
Genetic Insert: HES1
Vector Backbone Description: Backbone Size:11500; Vector Backbone:cFUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: mHES1 driven by the EF-1a promoter and a GFP reporter driven by the ubiquitin C (Ubc) promoter.
Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes.
Proper citation: RRID:Addgene_17625 Copy
Species: Mus musculus
Genetic Insert: TRIM46
Vector Backbone Description: Backbone Marker:unknown; Backbone Size:5962; Vector Backbone:pGW1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176266 Copy
Species: Mus musculus
Genetic Insert: Kir2.1MUT-P2A-EGFP
Vector Backbone Description: Backbone Marker:Vector Builder; Backbone Size:4638; Vector Backbone:pAAV-SynI; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
References:
Comments: Several differences were found flanking/near the lox and frt sites. These base changes do not affect the coding sequence or recombination sites.
Proper citation: RRID:Addgene_176279 Copy
Species: Mus musculus
Genetic Insert: t-complex-associated testis expressed 1
Vector Backbone Description: Backbone Size:5205; Vector Backbone:pCAG1.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176469 Copy
Species: Mus musculus
Genetic Insert: growth arrest specific 8
Vector Backbone Description: Backbone Size:5193; Vector Backbone:pCAG1.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176468 Copy
Species: Mus musculus
Genetic Insert: trim46
Vector Backbone Description: Vector Backbone:?pGW1-mCherry; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176401 Copy
Species: Mus musculus
Genetic Insert: Ascl1
Vector Backbone Description: Backbone Size:9471; Vector Backbone:Piggybac TetO - neomycin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176482 Copy
Species: Mus musculus
Genetic Insert: Tip47 (Delta 1-190)
Vector Backbone Description: Backbone Size:0; Vector Backbone:parallel HIS2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: The vector backbone, parallel HIS2, was made by replacing the polylinker of pET with the polylinker isolated from the baculovirus expression plasmid pFastBac (see PMID: 10024467).
Proper citation: RRID:Addgene_17640 Copy
Species: Mus musculus
Genetic Insert: RPA43
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4730; Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_17659 Copy
Species: Mus musculus
Genetic Insert: Cx43
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pGEM7Z; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: For in situ riboprobe.
Anti-sense: use T7; cut with BamHI or HindIII.
Sense: use Sp6; cut with XbaI.
Proper citation: RRID:Addgene_17668 Copy
Species: Mus musculus
Genetic Insert: Sam68
Vector Backbone Description: Backbone Marker:Amersham Pharmacia; Backbone Size:5000; Vector Backbone:pGEX2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: For additional information regarding the use of this plasmid, please see: Lin et al., J Biol Chem. 1997 Oct 24;272(43):27274-80. https://www.ncbi.nlm.nih.gov/pubmed/9341174
Proper citation: RRID:Addgene_17687 Copy
Species: Mus musculus
Genetic Insert: Sam68
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: pcDNA3 HA-Sam68 was generated by subcloning a BamHI/EcoRI insert from pGEX-Sam68 into pcDNA3-HA, which encodes amino-terminally hemagglutinin (HA) epitope-tagged proteins when cloned into its BamHI site.
Proper citation: RRID:Addgene_17688 Copy
Species: Mus musculus
Genetic Insert: Sam68
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_17689 Copy
Species: Mus musculus
Genetic Insert: Integrin-linked kinase
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.30.437490v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_176900 Copy
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