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  • Organism:homo sapiens (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pLpC-Cat-L-IFP-F[1]+Plum (mammalian)
 
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RRID:Addgene_52905 Cat-L-IFP-F[1]+Plum Homo sapiens Ampicillin PMID:24747815 Backbone Marker:Addgene; Vector Backbone:pLpC; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-01 01:14:38 0
pcDNA3-RPS6 (pDD1902)
 
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RRID:Addgene_52913 HAS6 Homo sapiens Ampicillin PMID:21734034 Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:14:38 0
pEF-Bos-FLAG MDA5 MI
 
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RRID:Addgene_52875 IFIH1 Homo sapiens Ampicillin PMID:19211564 Backbone Size:5500; Vector Backbone:pEF-Bos-Flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin K335A, E658K 2026-08-01 01:14:38 0
pLKO-shAUF1 hairpin 2
 
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RRID:Addgene_52922 shRNA targeting AUF1 Homo sapiens Ampicillin PMID:24670723 Vector Backbone:pLKO; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin 2026-08-01 01:14:38 0
pLKO-shp38
 
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1+ mentions
RRID:Addgene_52920 shRNA targeting mitogen-activated protein kinase p38 Homo sapiens Ampicillin PMID:24670723 Vector Backbone:pLKO; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin 2026-08-01 01:14:38 9
pSP64-hKCNQ1
 
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RRID:Addgene_53048 Potassium voltage-gated channel subfamily KQT member 1 Homo sapiens Ampicillin PMID:8900283 Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pSP64; Vector Types:Xenopus Oocyte Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:14:40 0
pSP64-hKCNE1
 
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RRID:Addgene_53049 Potassium voltage-gated channel subfamily E member 1 Homo sapiens Ampicillin PMID:8900283 Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pSP64; Vector Types:Xenopus Oocyte Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:14:40 0
pSP64-hERG1a
 
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1+ mentions
RRID:Addgene_53051 Potassium voltage-gated channel subfamily H member 2 Homo sapiens Ampicillin PMID:11005845 Backbone Marker:Promega; Backbone Size:3017; Vector Backbone:pSP64; Vector Types:Xenopus Oocyte Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:14:40 2
pCEP4-hKCNE1
 
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1+ mentions
RRID:Addgene_53050 Potassium voltage-gated channel subfamily E member 1 Homo sapiens Ampicillin PMID:8900283 Backbone Marker:Invitrogen; Backbone Size:10185; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:14:40 1
pSP64-hERG1a D540K
 
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RRID:Addgene_53057 Potassium voltage-gated channel subfamily H member 2 Homo sapiens Ampicillin PMID:9882738 Backbone Marker:Promega; Backbone Size:3017; Vector Backbone:pSP64; Vector Types:Xenopus Oocyte Expression; Bacterial Resistance:Ampicillin changed Aspartate 540 to Lysine 2026-08-01 01:14:40 0
pSP64-hERG1a S631A
 
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RRID:Addgene_53058 Potassium voltage-gated channel subfamily H member 2 Homo sapiens Ampicillin PMID:9547387 Backbone Marker:Promega; Backbone Size:3017; Vector Backbone:pSP64; Vector Types:Xenopus Oocyte Expression; Bacterial Resistance:Ampicillin changed Serine 631 to Alanine 2026-08-01 01:14:40 0
pSP64-hERG1a T623A
 
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1+ mentions
RRID:Addgene_53055 Potassium voltage-gated channel subfamily H member 2 Homo sapiens Ampicillin PMID:11005845 Backbone Marker:Promega; Backbone Size:3017; Vector Backbone:pSP64; Vector Types:Xenopus Oocyte Expression; Bacterial Resistance:Ampicillin changed Threonine 623 to Alanine 2026-08-01 01:14:40 1
pSP64-hERG1a F656A
 
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1+ mentions
RRID:Addgene_53054 Potassium voltage-gated channel subfamily H member 2 Homo sapiens Ampicillin PMID:11005845 Backbone Marker:Promega; Backbone Size:3017; Vector Backbone:pSP64; Vector Types:Xenopus Oocyte Expression; Bacterial Resistance:Ampicillin changed Phenylalanine 656 to Alanine 2026-08-01 01:14:40 1
pLenti-OC-IRES-BSD
 
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RRID:Addgene_53118 OCT1 Homo sapiens Ampicillin PMID:24717434 Vector Backbone:pLenti-CMV-BSD; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-01 01:14:40 0
pGfa2-nLac
 
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RRID:Addgene_53126 Gfa2 Homo sapiens Ampicillin PMID:8120611 The plasmid is a pUC18 vector containing the human GFAP sequences from -2163 to +47 (the human gfa2 segment), with the initiating ATG mutated to TTG, placed in front of the nuclear targeted E. coli lacZ gene, which in turn is followed by a fragment of the mouse protamine-1 gene. The latter supplies an intron, stabilizing 3' UTR, and a polyadenylation signal. If cloning a cDNA, it is advisable to retain the mP-1 segment. The lacZ gene can be excised by digestion with BamHI, and replaced with your gene of interest. If cloning a genomic sequence that includes an intron and polyadenylation site (this may compromise astrocyte specificity--see Su et al. 2004), the mP-1 region can also be excised, or alternatively, a number of sites flank the gfa2 segment allowing its isolation. Restriction sites that may be useful are as follows: EcoRI: flanks the gfa2, lacZ, and mP-1 segments Bgl II: 5' flank of gfa2 & 3' flank of mP-1 Bam HI: flanks the lacZ gene Sal I: cuts uniquely between the gfa2 promoter and the LacZ gene Sph I and Hind III: cut uniquely just 3' of the mP-1 segment Although the GFAP promoter appears to be the best choice for targeting transgene expression to astrocytes in mice, please be aware that neuronal expression has also occasionally been observed (Su et al. 2004). Vector Backbone:pUC18; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin contains -2163 to +47 (the human gfa2 segment), with the initiating ATG mutated to TTG 2026-08-01 01:14:41 5
pGfa(B3)-nLac
 
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RRID:Addgene_53132 Gfa2 Homo sapiens Ampicillin PMID:16496373 This plasmid is a variant of pGfa2-nLac (Addgene plasmid #53126) constructed by inserting 3 copies of the gfa2 B segment (Besnard et al. 1991) into the Sma I site just upstream of the basal promoter. It has about 75 times higher activity than pGfa2-nLac in transfected cells, but does not work in transgenic mice; possibly the high level of expression it produces is toxic. Vector Backbone:pUC18; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin -2163 to +47, with 3 copies of the gfa2 B segment inserted into the Sma I site just upstream of the basal promoter, with the initiating ATG mutated to TTG 2026-08-01 01:14:41 0
CD59-bio-His
 
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RRID:Addgene_53154 CD59 Homo sapiens Ampicillin PMID:25713122 Article ref 86 Backbone Marker:Yves Durocher, NRC; Backbone Size:6651; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:14:41 0
pWZLblasti-HA-MEK1
 
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1+ mentions
RRID:Addgene_53161 MEK1 Homo sapiens Ampicillin PMID:24717435 Backbone Size:5100; Vector Backbone:pWZLblasti; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-01 01:14:41 1
pGEX4T3-HA-ERK2 D321N
 
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RRID:Addgene_53202 ERK2 Homo sapiens Ampicillin PMID:24717435 The sequence discrepancies found during the QC process have no functional consequence. Backbone Marker:GE Healthcare; Backbone Size:4968; Vector Backbone:pGEX4T3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin D321N 2026-08-01 01:14:42 0
pWZLblasti-HA-MEK5
 
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RRID:Addgene_53167 MEK5 Homo sapiens Ampicillin PMID:24717435 The sequence discrepancies found during the QC process have no functional consequence. Backbone Size:5100; Vector Backbone:pWZLblasti; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-01 01:14:42 0

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