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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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|---|---|---|---|---|---|---|---|---|---|---|
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pLpC-Cat-L-IFP-F[1]+Plum (mammalian) Resource Report Resource Website |
RRID:Addgene_52905 | Cat-L-IFP-F[1]+Plum | Homo sapiens | Ampicillin | PMID:24747815 | Backbone Marker:Addgene; Vector Backbone:pLpC; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-01 01:14:38 | 0 | ||
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pcDNA3-RPS6 (pDD1902) Resource Report Resource Website |
RRID:Addgene_52913 | HAS6 | Homo sapiens | Ampicillin | PMID:21734034 | Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-01 01:14:38 | 0 | ||
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pEF-Bos-FLAG MDA5 MI Resource Report Resource Website |
RRID:Addgene_52875 | IFIH1 | Homo sapiens | Ampicillin | PMID:19211564 | Backbone Size:5500; Vector Backbone:pEF-Bos-Flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | K335A, E658K | 2026-08-01 01:14:38 | 0 | |
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pLKO-shAUF1 hairpin 2 Resource Report Resource Website |
RRID:Addgene_52922 | shRNA targeting AUF1 | Homo sapiens | Ampicillin | PMID:24670723 | Vector Backbone:pLKO; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin | 2026-08-01 01:14:38 | 0 | ||
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pLKO-shp38 Resource Report Resource Website 1+ mentions |
RRID:Addgene_52920 | shRNA targeting mitogen-activated protein kinase p38 | Homo sapiens | Ampicillin | PMID:24670723 | Vector Backbone:pLKO; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin | 2026-08-01 01:14:38 | 9 | ||
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pSP64-hKCNQ1 Resource Report Resource Website |
RRID:Addgene_53048 | Potassium voltage-gated channel subfamily KQT member 1 | Homo sapiens | Ampicillin | PMID:8900283 | Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pSP64; Vector Types:Xenopus Oocyte Expression; Bacterial Resistance:Ampicillin | 2026-08-01 01:14:40 | 0 | ||
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pSP64-hKCNE1 Resource Report Resource Website |
RRID:Addgene_53049 | Potassium voltage-gated channel subfamily E member 1 | Homo sapiens | Ampicillin | PMID:8900283 | Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pSP64; Vector Types:Xenopus Oocyte Expression; Bacterial Resistance:Ampicillin | 2026-08-01 01:14:40 | 0 | ||
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pSP64-hERG1a Resource Report Resource Website 1+ mentions |
RRID:Addgene_53051 | Potassium voltage-gated channel subfamily H member 2 | Homo sapiens | Ampicillin | PMID:11005845 | Backbone Marker:Promega; Backbone Size:3017; Vector Backbone:pSP64; Vector Types:Xenopus Oocyte Expression; Bacterial Resistance:Ampicillin | 2026-08-01 01:14:40 | 2 | ||
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pCEP4-hKCNE1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_53050 | Potassium voltage-gated channel subfamily E member 1 | Homo sapiens | Ampicillin | PMID:8900283 | Backbone Marker:Invitrogen; Backbone Size:10185; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-01 01:14:40 | 1 | ||
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pSP64-hERG1a D540K Resource Report Resource Website |
RRID:Addgene_53057 | Potassium voltage-gated channel subfamily H member 2 | Homo sapiens | Ampicillin | PMID:9882738 | Backbone Marker:Promega; Backbone Size:3017; Vector Backbone:pSP64; Vector Types:Xenopus Oocyte Expression; Bacterial Resistance:Ampicillin | changed Aspartate 540 to Lysine | 2026-08-01 01:14:40 | 0 | |
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pSP64-hERG1a S631A Resource Report Resource Website |
RRID:Addgene_53058 | Potassium voltage-gated channel subfamily H member 2 | Homo sapiens | Ampicillin | PMID:9547387 | Backbone Marker:Promega; Backbone Size:3017; Vector Backbone:pSP64; Vector Types:Xenopus Oocyte Expression; Bacterial Resistance:Ampicillin | changed Serine 631 to Alanine | 2026-08-01 01:14:40 | 0 | |
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pSP64-hERG1a T623A Resource Report Resource Website 1+ mentions |
RRID:Addgene_53055 | Potassium voltage-gated channel subfamily H member 2 | Homo sapiens | Ampicillin | PMID:11005845 | Backbone Marker:Promega; Backbone Size:3017; Vector Backbone:pSP64; Vector Types:Xenopus Oocyte Expression; Bacterial Resistance:Ampicillin | changed Threonine 623 to Alanine | 2026-08-01 01:14:40 | 1 | |
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pSP64-hERG1a F656A Resource Report Resource Website 1+ mentions |
RRID:Addgene_53054 | Potassium voltage-gated channel subfamily H member 2 | Homo sapiens | Ampicillin | PMID:11005845 | Backbone Marker:Promega; Backbone Size:3017; Vector Backbone:pSP64; Vector Types:Xenopus Oocyte Expression; Bacterial Resistance:Ampicillin | changed Phenylalanine 656 to Alanine | 2026-08-01 01:14:40 | 1 | |
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pLenti-OC-IRES-BSD Resource Report Resource Website |
RRID:Addgene_53118 | OCT1 | Homo sapiens | Ampicillin | PMID:24717434 | Vector Backbone:pLenti-CMV-BSD; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-01 01:14:40 | 0 | ||
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pGfa2-nLac Resource Report Resource Website 1+ mentions |
RRID:Addgene_53126 | Gfa2 | Homo sapiens | Ampicillin | PMID:8120611 | The plasmid is a pUC18 vector containing the human GFAP sequences from -2163 to +47 (the human gfa2 segment), with the initiating ATG mutated to TTG, placed in front of the nuclear targeted E. coli lacZ gene, which in turn is followed by a fragment of the mouse protamine-1 gene. The latter supplies an intron, stabilizing 3' UTR, and a polyadenylation signal. If cloning a cDNA, it is advisable to retain the mP-1 segment. The lacZ gene can be excised by digestion with BamHI, and replaced with your gene of interest. If cloning a genomic sequence that includes an intron and polyadenylation site (this may compromise astrocyte specificity--see Su et al. 2004), the mP-1 region can also be excised, or alternatively, a number of sites flank the gfa2 segment allowing its isolation. Restriction sites that may be useful are as follows: EcoRI: flanks the gfa2, lacZ, and mP-1 segments Bgl II: 5' flank of gfa2 & 3' flank of mP-1 Bam HI: flanks the lacZ gene Sal I: cuts uniquely between the gfa2 promoter and the LacZ gene Sph I and Hind III: cut uniquely just 3' of the mP-1 segment Although the GFAP promoter appears to be the best choice for targeting transgene expression to astrocytes in mice, please be aware that neuronal expression has also occasionally been observed (Su et al. 2004). | Vector Backbone:pUC18; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin | contains -2163 to +47 (the human gfa2 segment), with the initiating ATG mutated to TTG | 2026-08-01 01:14:41 | 5 |
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pGfa(B3)-nLac Resource Report Resource Website |
RRID:Addgene_53132 | Gfa2 | Homo sapiens | Ampicillin | PMID:16496373 | This plasmid is a variant of pGfa2-nLac (Addgene plasmid #53126) constructed by inserting 3 copies of the gfa2 B segment (Besnard et al. 1991) into the Sma I site just upstream of the basal promoter. It has about 75 times higher activity than pGfa2-nLac in transfected cells, but does not work in transgenic mice; possibly the high level of expression it produces is toxic. | Vector Backbone:pUC18; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin | -2163 to +47, with 3 copies of the gfa2 B segment inserted into the Sma I site just upstream of the basal promoter, with the initiating ATG mutated to TTG | 2026-08-01 01:14:41 | 0 |
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CD59-bio-His Resource Report Resource Website |
RRID:Addgene_53154 | CD59 | Homo sapiens | Ampicillin | PMID:25713122 | Article ref 86 | Backbone Marker:Yves Durocher, NRC; Backbone Size:6651; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-01 01:14:41 | 0 | |
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pWZLblasti-HA-MEK1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_53161 | MEK1 | Homo sapiens | Ampicillin | PMID:24717435 | Backbone Size:5100; Vector Backbone:pWZLblasti; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-01 01:14:41 | 1 | ||
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pGEX4T3-HA-ERK2 D321N Resource Report Resource Website |
RRID:Addgene_53202 | ERK2 | Homo sapiens | Ampicillin | PMID:24717435 | The sequence discrepancies found during the QC process have no functional consequence. | Backbone Marker:GE Healthcare; Backbone Size:4968; Vector Backbone:pGEX4T3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | D321N | 2026-08-01 01:14:42 | 0 |
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pWZLblasti-HA-MEK5 Resource Report Resource Website |
RRID:Addgene_53167 | MEK5 | Homo sapiens | Ampicillin | PMID:24717435 | The sequence discrepancies found during the QC process have no functional consequence. | Backbone Size:5100; Vector Backbone:pWZLblasti; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-01 01:14:42 | 0 |
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