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Species: Homo sapiens
Genetic Insert: BRD4 long isoform without its C-terminal, P-TEFb interacting domain
Vector Backbone Description: Backbone Size:8400; Vector Backbone:pCW57-GFP-2A-MCS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32966794
Proper citation: RRID:Addgene_137722 Copy
Species: Homo sapiens
Genetic Insert: CCR5 Promoter
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL-3 Basic Vector; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28331180
Comments: pGL-3 basic vector (Promega) can be used as a control to provide baseline luciferase activity
Proper citation: RRID:Addgene_137699 Copy
Species: Homo sapiens
Genetic Insert: U1A (1-94)
Vector Backbone Description: Backbone Size:4967; Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11142374
Comments: CEN plasmid, GAL1 promoter
Proper citation: RRID:Addgene_1376 Copy
Species: Homo sapiens
Genetic Insert: MAP1LC3C
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31971854
Proper citation: RRID:Addgene_137758 Copy
Species: Homo sapiens
Genetic Insert: MAP1LC3B
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31971854
Comments: 3' codons have been altered from original reference sequence, while preserving the amino acid sequence.
Proper citation: RRID:Addgene_137757 Copy
Species: Homo sapiens
Genetic Insert: GABARAPL1
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31971854
Proper citation: RRID:Addgene_137759 Copy
Species: Homo sapiens
Genetic Insert: GABARAP
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31971854
Proper citation: RRID:Addgene_137760 Copy
Species: Homo sapiens
Genetic Insert: CALCOCO1
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31971854
Proper citation: RRID:Addgene_137765 Copy
Species: Homo sapiens
Genetic Insert: CALCOCO1
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31971854
Proper citation: RRID:Addgene_137766 Copy
Species: Homo sapiens
Genetic Insert: CALCOCO1 W47A
Vector Backbone Description: Vector Backbone:pCMV6-Entry; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31971854
Proper citation: RRID:Addgene_137769 Copy
Species: Homo sapiens
Genetic Insert: ITPKA
Vector Backbone Description: Vector Backbone:pEGFP-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_137811 Copy
Species: Homo sapiens
Genetic Insert: CD44v3 (-cyt)
Vector Backbone Description: Backbone Marker:lab Guenthert; Backbone Size:4320; Vector Backbone:mini pBR322; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16167069
Proper citation: RRID:Addgene_137813 Copy
Species: Homo sapiens
Genetic Insert: CD44s (fl)
Vector Backbone Description: Backbone Marker:lab Guenthert; Backbone Size:4320; Vector Backbone:mini pBR322; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16167069
Comments: Addgene NGS result found mutations I186T and I253V when compared to CD44 [XP_016874074.1]. Depositor confirmed that this does not affect the function of the plasmid.
Proper citation: RRID:Addgene_137812 Copy
Species: Homo sapiens
Genetic Insert: CD44v3 (fl)
Vector Backbone Description: Backbone Marker:lab Guenthert; Backbone Size:4320; Vector Backbone:mini pBR322; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16167069
Proper citation: RRID:Addgene_137814 Copy
Species: Homo sapiens
Genetic Insert: CD44v6 (fl)
Vector Backbone Description: Backbone Marker:lab Guenthert; Backbone Size:4320; Vector Backbone:mini pBR322; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16167069
Comments: R296K in the V6 region is not a concern to the plasmid function.
Proper citation: RRID:Addgene_137816 Copy
Species: Homo sapiens
Genetic Insert: Human GRK6[L66A E514A]
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32095688
Proper citation: RRID:Addgene_137781 Copy
Species: Homo sapiens
Genetic Insert: Human GRK6
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32095688
Proper citation: RRID:Addgene_137780 Copy
Species: Homo sapiens
Genetic Insert: mir30 cassette
Vector Backbone Description: Backbone Size:5555; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17209010
Comments: Cre recombinase-dependent inducible RNAi vector. shRNA is expressed only in the presence of Cre.
Haipin DNA can be cloned into the XhoI/EcoRI site. A protocol for hairpin DNA design and cloning was reported by Gregory Hannon lab http://hannonlab.cshl.edu/publications/shrt_hrpin_RNA.pdf (Paddison et al. Nat. Methods 1, 163-167 (2004)).
Addgene's quality control sequencing finds a few nucleotides of discrepancy with the depositor's provided full plasmid sequence. These changes are not thought to affect plasmid function.
Proper citation: RRID:Addgene_13786 Copy
Species: Homo sapiens
Genetic Insert: Human beta2-adrenergic receptor
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32095688
Proper citation: RRID:Addgene_137785 Copy
Species: Homo sapiens
Genetic Insert: CD44v6-10 (fl)
Vector Backbone Description: Backbone Marker:lab Guenthert; Backbone Size:4320; Vector Backbone:mini pBR322; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16167069
Proper citation: RRID:Addgene_137820 Copy
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