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Species: Homo sapiens
Genetic Insert: PRAS40
Vector Backbone Description: Backbone Size:4754; Vector Backbone:pRK5-Myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_15476 Copy
Species: Homo sapiens
Genetic Insert: hNET
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_15475 Copy
Species: Homo sapiens
Genetic Insert: ZIM3
Vector Backbone Description: Backbone Marker:David Root lab; Addgene plasmid #25895; Vector Backbone:pLX301; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_154761 Copy
Species: Homo sapiens
Genetic Insert: coEPO
Vector Backbone Description: Backbone Marker:DNA2.0; Backbone Size:2703; Vector Backbone:pJ204; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
References:
Comments: This is a donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE) using poly(A)-trap.
Promoter 100RPU.2 was initially described in:
Brown et al. 2017 "In silico design of context-responsive mammalian promoters with user-defined functionality." DOI: 10.1093/nar/gkx768
A patent application has been filed by third parties on the promoter 100RPU.2 used in this plasmid, permission for deposition of plasmids containing the promoter sequence has been provided by AstraZeneca and The University of Sheffield.
The plasmid was assembled by USER cloning.
Proper citation: RRID:Addgene_154829 Copy
Species: Homo sapiens
Genetic Insert: Stx5 (Long isoform)
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRES_neo3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_154846 Copy
Species: Homo sapiens
Genetic Insert: PRAS40
Vector Backbone Description: Backbone Size:4754; Vector Backbone:pRK5-Myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_15479 Copy
Species: Homo sapiens
Genetic Insert: TSC2 shRNA
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
References:
Comments: Target sequence for human TSC2 is 5'-CACTGGCCTTGGACGGTATTG-3'.
Proper citation: RRID:Addgene_15478 Copy
Species: Homo sapiens
Genetic Insert: XYLT2 sgRNA
Vector Backbone Description: Vector Backbone:lentiCRISPR v2 (addgene# 52961); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_154862 Copy
Species: Homo sapiens
Genetic Insert: SLC35B2
Vector Backbone Description: Vector Backbone:pLJM1-EGFP (Addgene# 19319); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: Please note: Plasmid contains an A291V mutation in SLC35B2 compared to the NCBI reference sequence NM_178148. This mutation is not expected to affect plasmid function as the plasmid was still able to rescue the heparan synthesis and endocytosis defects of SLC35B2 knockout cells.
Proper citation: RRID:Addgene_154863 Copy
Species: Homo sapiens
Genetic Insert: MYO7B sgRNA
Vector Backbone Description: Vector Backbone:lentiCRISPR v2 (addgene# 52961); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_154861 Copy
Species: Homo sapiens
Genetic Insert: shRNA and amiRNA against YY1
Vector Backbone Description: Backbone Marker:Constructed from pGreenPuro (SBI) by Magnus Essand Lab; Vector Backbone:pBMN(CMV-copGFP-Puro); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_154943 Copy
Species: Homo sapiens
Genetic Insert: Phosphoglycerate dehydrogenase
Vector Backbone Description: Backbone Size:7943; Vector Backbone:pCW57-MCS1-P2A-MCS2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_154903 Copy
Species: Homo sapiens
Genetic Insert: SORL1
Vector Backbone Description: Backbone Marker:Addgene, #73638; Backbone Size:5211; Vector Backbone:pDEST-ORF-V2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_154900 Copy
Species: Homo sapiens
Genetic Insert: L1-W1036L
Vector Backbone Description: Backbone Size:5450; Vector Backbone:pcDNA3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_15488 Copy
Species: Homo sapiens
Genetic Insert: PHGDH, codon optimized
Vector Backbone Description: Backbone Size:5600; Vector Backbone:pMXS-IRES-blast; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_154915 Copy
Species: Homo sapiens
Genetic Insert: PHGDH
Vector Backbone Description: Backbone Size:15200; Vector Backbone:pCW57.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_154916 Copy
Species: Homo sapiens
Genetic Insert: KLF2-2A-NANOG
Vector Backbone Description: Vector Backbone:pCE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_154879 Copy
Species: Homo sapiens
Genetic Insert: PFAS
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_154912 Copy
Species: Homo sapiens
Genetic Insert: KDM4D, GLIS1
Vector Backbone Description: Vector Backbone:pCE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_154880 Copy
Species: Homo sapiens
Genetic Insert: ASCL1
Vector Backbone Description: Vector Backbone:CSIV-TRE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_154883 Copy
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