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Species: Homo sapiens
Genetic Insert: LZIP
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:p3XFlag-CMV-7.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_32960 Copy
Species: Homo sapiens
Genetic Insert: Separase
Vector Backbone Description: Backbone Marker:RZPD; Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11747808
Proper citation: RRID:Addgene_33016 Copy
Species: Homo sapiens
Genetic Insert: MEF2 binding sites
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5000; Vector Backbone:pGL3-promoter modified; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Comments: This plasmid was cloned from pFOS WT-GL3 (Addgene #11983), where the human c-fos promoter was removed and replaced with 4 MEF2 sites. See author's map for restriction site/MCS details.
Proper citation: RRID:Addgene_32967 Copy
Species: Homo sapiens
Genetic Insert: Tbx5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2700; Vector Backbone:pEntr3c; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21415370
Proper citation: RRID:Addgene_32968 Copy
Species: Homo sapiens
Genetic Insert: H2B-PAGFP
Vector Backbone Description: Backbone Size:4771; Vector Backbone:N/A; Vector Types:Mammalian Expression, AAV, adeno associated virus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22144948
Comments: Plasmid containing the H2B-PAGFP fusion gene was a gift from André Nussenzweig (Kruhlak et al., 2006). The H2B-PAGFP gene was subcloned into the pACAGW-ChR2-Venus-AAV plasmid (Addgene # 20071).
Kruhlak, M. J., Celeste, A., Dellaire, G., Fernandez-Capetillo, O., Müller, W. G., McNally, J. G., Bazett-Jones, D. P., and Nussenzweig, A. (2006). Changes in chromatin structure and mobility in living cells at sites of DNA double-strand breaks. J. Cell Biol. 172, 823-34.
There is a 5bp deletion in Addgene's sequence compared to depositor's reference sequence. The deletion is in the vector sequence upstream of the ORF and does not affect plasmid function. There is also a mutation D26G in H2B; this mutation is found in depositor's reference sequence as well.
Proper citation: RRID:Addgene_33000 Copy
Species: Homo sapiens
Genetic Insert: ATF6
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11821395
Comments: During sequencing a change of Methionine 67 to Leucine, and Methionine 313 to Isoleucine was detected. This change in the basic region should not affect experiments with this construct, but could affect DNA binding by derivatives made from this plasmid.
Proper citation: RRID:Addgene_32956 Copy
Species: Homo sapiens
Genetic Insert: S2P
Vector Backbone Description: Backbone Marker:M. Tanaka; Backbone Size:7500; Vector Backbone:pCGN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12110171
Proper citation: RRID:Addgene_32957 Copy
Species: Homo sapiens
Genetic Insert: E6AP
Vector Backbone Description: Vector Backbone:pcDNA4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15263005
Proper citation: RRID:Addgene_32892 Copy
Species: Homo sapiens
Genetic Insert: E6AP
Vector Backbone Description: Vector Backbone:pcDNA4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15263005
Proper citation: RRID:Addgene_32890 Copy
Species: Homo sapiens
Genetic Insert: E6AP
Vector Backbone Description: Vector Backbone:pcDNA4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15263005
Proper citation: RRID:Addgene_32891 Copy
Species: Homo sapiens
Genetic Insert: Histone H1.1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15911621
Proper citation: RRID:Addgene_32894 Copy
Species: Homo sapiens
Genetic Insert: Brd4
Vector Backbone Description: Vector Backbone:MSCV-CMV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21555454
Proper citation: RRID:Addgene_32886 Copy
Species: Homo sapiens
Genetic Insert: SOS1
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCGT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17084704
Proper citation: RRID:Addgene_32923 Copy
Species: Homo sapiens
Genetic Insert: SOS2
Vector Backbone Description: Backbone Marker:M. Tanaka; Backbone Size:7500; Vector Backbone:pCGN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7629138
Proper citation: RRID:Addgene_32921 Copy
Species: Homo sapiens
Genetic Insert: HECW2
Vector Backbone Description: Backbone Marker:Structural Genomics Consortium (Addgene plasmid 26096); Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 2LFE. http://www.thesgc.org/structures/2LFE/
Proper citation: RRID:Addgene_32875 Copy
Species: Homo sapiens
Genetic Insert: RAB3B
Vector Backbone Description: Backbone Marker:Structural Genomics Consortium (Addgene plasmid 26096); Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3DZ8. http://www.thesgc.org/structures/3DZ8/
Proper citation: RRID:Addgene_32879 Copy
Species: Homo sapiens
Genetic Insert: Tubulin
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20133936
Comments: This is the acetylation-mimicking mutant.
Proper citation: RRID:Addgene_32912 Copy
Species: Homo sapiens
Genetic Insert: hG9a
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15269344
Proper citation: RRID:Addgene_33025 Copy
Species: Homo sapiens
Genetic Insert: hG9a
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15269344
Proper citation: RRID:Addgene_33022 Copy
Species: Homo sapiens
Genetic Insert: hG9a
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15269344
Proper citation: RRID:Addgene_33023 Copy
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