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Species: Mus musculus
Genetic Insert: pAAV CAG iGluSnFR3 v857.GPI
Vector Backbone Description: Backbone Size:4836; Vector Backbone:pAAV CAG; Vector Types:Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_178335 Copy
Species: Mus musculus
Genetic Insert: pAAV GFAP iGluSnFR3 v857.PDGFR
Vector Backbone Description: Backbone Size:4852; Vector Backbone:pAAV GFAP; Vector Types:Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_178336 Copy
Species: Mus musculus
Genetic Insert: Twist
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_1783 Copy
Species: Mus musculus
Genetic Insert: siTwist
Vector Backbone Description: Backbone Size:9439; Vector Backbone:pSP-108; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Twist-siRNA3-targetting sequence is AAGCTGAGCAAGATTCAGACC
Proper citation: RRID:Addgene_1784 Copy
Species: Mus musculus
Genetic Insert: SHP2-EGFP
Vector Backbone Description: Backbone Marker:Chen lab; Backbone Size:7600; Vector Backbone:MDH1-454; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: This construct simultaneously expresses miR-181a and the SHP2 gene.
Proper citation: RRID:Addgene_17851 Copy
Species: Mus musculus
Genetic Insert: H-2Kb
Vector Backbone Description: Backbone Marker:Chen lab; Backbone Size:7200; Vector Backbone:MDH1-404; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: The original retroviral miRNA expression construct (Chen et al., 2004) was modified
to incorporate a truncated H-2Kb surface marker and a blasticidin drug resistance gene to allow for magnetic bead assisted sorting and drug selection. These
marker genes are driven by the PGK promoter and transcribed as a bicistronic
message, with the truncated H-2Kb gene placed after the PGK promoter and the
blasticidin resistance gene after the EMCV IRES.
MiRNA gene expression is driven by the human H1 pol III promoter. The truncated H-2Kb selection marker is non-functional since it is devoid of a cytoplasmic domain and is of a different MHC haplotype than the T cells in 5C.C7 transgenic mice (H-2Kk).
Proper citation: RRID:Addgene_17852 Copy
Species: Mus musculus
Genetic Insert: NFATc2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCR2.1-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Full-length mNFATc2
Proper citation: RRID:Addgene_17858 Copy
Species: Mus musculus
Genetic Insert: GlyCAM-1/IgG
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4356; Vector Backbone:pCDM8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_17841 Copy
Species: Mus musculus
Genetic Insert: Dusp6-EGFP
Vector Backbone Description: Backbone Marker:Chen lab; Backbone Size:7600; Vector Backbone:MDH1-454; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: This construct simultaneously expresses miR-181a and the DUSP6 gene.
Proper citation: RRID:Addgene_17849 Copy
Species: Mus musculus
Genetic Insert: Txnip
Vector Backbone Description: Vector Backbone:pFUW-TetO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: Please note: Plasmid contains a M172K mutation in mouse Txnip compared to the NCBI reference sequence NP_001009935.1. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_178437 Copy
Species: Mus musculus
Genetic Insert: Irf7
Vector Backbone Description: Vector Backbone:pFUW-TetO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_178445 Copy
Species: Mus musculus
Genetic Insert: Junb
Vector Backbone Description: Vector Backbone:pFUW-TetO; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_178441 Copy
Species: Mus musculus
Genetic Insert: CnB
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pBJ5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_17872 Copy
Species: Mus musculus
Genetic Insert: mCherry-Zdk1-Vav2(DH-PH-CRD)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4679; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_178875 Copy
Species: Mus musculus
Genetic Insert: PB1-AzamiGreen-FRB-TEVcs(ENLYFQL)-mTagBFP2-HA-Vav2(DH-PH-CRD)
Vector Backbone Description: Backbone Marker:MBL International; Backbone Size:4244; Vector Backbone:pAsh-MCL; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_178870 Copy
Species: Mus musculus
Genetic Insert: FKBP-mCherry-Vav2(DH-PH-CRD)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3940; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_178865 Copy
Species: Mus musculus
Genetic Insert: protein phosphatase 3, regulatory subunit B, beta isoform
Vector Backbone Description: Backbone Size:5142; Vector Backbone:pCAG1.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_179161 Copy
Species: Mus musculus
Genetic Insert: spermatogenesis associated 33
Vector Backbone Description: Backbone Size:5211; Vector Backbone:pCAG1.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_179129 Copy
Species: Mus musculus
Genetic Insert: spermatogenesis associated 33
Vector Backbone Description: Backbone Size:5211; Vector Backbone:pCAG1.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_179130 Copy
Species: Mus musculus
Genetic Insert: Histone H3
Vector Backbone Description: Vector Backbone:pECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: EcoRI-XhoI fragment of pZErO-histone H3 (Tachibana et al. 2001 https://doi.org/10.1074/jbc.m101914200)
Proper citation: RRID:Addgene_179698 Copy
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