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Species: Homo sapiens
Genetic Insert: EZH2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note that there is an additional mutation in EZH2 ORF, Q3R. The mutation does not affect plasmid function.
Proper citation: RRID:Addgene_42664 Copy
Species: Homo sapiens
Genetic Insert: EGFR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA6/myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_42665 Copy
Species: Homo sapiens
Genetic Insert: NKX3.1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This is a full length NKX3.1 cDNA construct.
Proper citation: RRID:Addgene_42897 Copy
Species: Homo sapiens
Genetic Insert: protein kinase R
Vector Backbone Description: Vector Backbone:pet11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_42934 Copy
Species: Homo sapiens
Genetic Insert: NKX3.1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET-30a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: This is a full length NKX3.1 cDNA construct. Protein expressed in E.coli BL21(DE3) competent cells
Proper citation: RRID:Addgene_42896 Copy
Species: Homo sapiens
Genetic Insert: PECAM
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pcDNAI Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_42902 Copy
Species: Homo sapiens
Genetic Insert: Cry2PHR-mCherry-LRP6c
Vector Backbone Description: Backbone Size:7788; Vector Backbone:CLPIT; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_42961 Copy
Species: Homo sapiens
Genetic Insert: Cry2PHR-mCherry-RhoA
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3991; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_42958 Copy
Species: Homo sapiens
Genetic Insert: Cry2PHR-mCherry-RhoA
Vector Backbone Description: Backbone Size:7821; Vector Backbone:CLPIT; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_42959 Copy
Species: Homo sapiens
Genetic Insert: Negative control Functionally Dead Human L1 element
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10410; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: These plasmids consist of the L1RP element tagged with an enhanced green fluorescent protein (EGFP) cassette (pL1RP-EGFP) in a pCEP4 backbone (Invitrogen). The L1 element is driven by its 5′-UTR and an upstream CMV MIE promoter. L1-EGFP (EF06R, Addgene plasmid #42940) was derived from L1RP-EGFP by blunt ligation of 1–1392 nt of pPur (BD/Clontech) into the NruI site of pCEP4.
A negative control ‘dead’ L1-EGFP (EF05J, Addgene plasmid #42941) was similarly derived from pL1RP(JM111)-EGFP, which contains disabling mutations in ORF1.
An EN− plasmid (EF13E, Addgene plasmid #42942) was created by swapping 1927–3708 nt (Age I–Bcl I) from JM102D205A, which contains an endonuclease function-abrogating D205A point mutation in the EN domain, into EF06R.
A control plasmid (EF12J, Addgene plasmid #42943) was similarly derived from JM102. JM102 and JM102D205A are derived from L1.3, which differs from L1RP by 10 base changes in the region swapped.
The A250V mutation found during Addgene QC is conservative and should not affect function.
Proper citation: RRID:Addgene_42941 Copy
Species: Homo sapiens
Genetic Insert: Human L1 element
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10410; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: These plasmids consist of the L1RP element tagged with an enhanced green fluorescent protein (EGFP) cassette (pL1RP-EGFP) in a pCEP4 backbone (Invitrogen). The L1 element is driven by its 5′-UTR and an upstream CMV MIE promoter. L1-EGFP (EF06R, Addgene plasmid #42940) was derived from L1RP-EGFP by blunt ligation of 1–1392 nt of pPur (BD/Clontech) into the NruI site of pCEP4.
A negative control ‘dead’ L1-EGFP (EF05J, Addgene plasmid #42941) was similarly derived from pL1RP(JM111)-EGFP, which contains disabling mutations in ORF1.
An EN− plasmid (EF13E, Addgene plasmid #42942) was created by swapping 1927–3708 nt (Age I–Bcl I) from JM102D205A, which contains an endonuclease function-abrogating D205A point mutation in the EN domain, into EF06R.
A control plasmid (EF12J, Addgene plasmid #42943) was similarly derived from JM102. JM102 and JM102D205A are derived from L1.3, which differs from L1RP by 10 base changes in the region swapped.
I->T substitution at the end of ORF 2 should not affect plasmid function.
Proper citation: RRID:Addgene_42940 Copy
Species: Homo sapiens
Genetic Insert: SMARCA4
Vector Backbone Description: Backbone Size:7582; Vector Backbone:PiggyBac transposon; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_153949 Copy
Species: Homo sapiens
Genetic Insert: SMARCA4
Vector Backbone Description: Backbone Size:7582; Vector Backbone:PiggyBac transposon; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_153948 Copy
Species: Homo sapiens
Genetic Insert: CD55 (a 1,016-bp fragment from intron 3, exon 4, and intron 4)
Vector Backbone Description: Backbone Marker:Masahito Ikawa; Backbone Size:6383; Vector Backbone:pCAG-EGxxFP (Addgene #50716); Vector Types:Mammalian Expression, A plasmid specific for EGxxFP assay; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_153959 Copy
Species: Homo sapiens
Genetic Insert: acGFP-Sec61
Vector Backbone Description: Vector Backbone:pLV-CMV-EF1a-IRES-Blast; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_154009 Copy
Species: Homo sapiens
Genetic Insert: PIGA (a 401-bp fragment from intron 5 and exon 6)
Vector Backbone Description: Backbone Marker:Masahito Ikawa; Backbone Size:6383; Vector Backbone:pCAG-EGxxFP (Addgene #50716); Vector Types:Mammalian Expression, A plasmid specific for EGxxFP assay; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_153958 Copy
Species: Homo sapiens
Genetic Insert: IRAK1
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pCMV6-Entry; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_153968 Copy
Species: Homo sapiens
Genetic Insert: Axin1
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:6716; Vector Backbone:pMBP-MG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_154012 Copy
Species: Homo sapiens
Genetic Insert: GSK3B
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:6716; Vector Backbone:pMBP-MG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_154010 Copy
Species: Homo sapiens
Genetic Insert: MED17
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5700; Vector Backbone:pET19b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_15412 Copy
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