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Species: Mus musculus
Genetic Insert: PGC1a
Vector Backbone Description: Backbone Marker:Jeng-Shin Lee, Harvard University; Vector Backbone:AAV-GFP; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments: Flag-PGC1a-6His sequence was obtained from Bruce Spiegelman Lab. Note that there is a Q339K mutation relative to the canonical GenBank sequence. This mutation has not been reported to affect function.
Proper citation: RRID:Addgene_67637 Copy
Species: Mus musculus
Genetic Insert: sgNeo
Vector Backbone Description: Vector Backbone:pLL3.3; Vector Types:Mammalian Expression, Mouse Targeting, Lentiviral, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_67594 Copy
Species: Mus musculus
Genetic Insert: SOD-2A-Catalase
Vector Backbone Description: Backbone Marker:Jeng-Shin Lee, Harvard University; Vector Backbone:AAV-MCS8; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_67635 Copy
Species: Mus musculus
Genetic Insert: 2 binding sites for miR-141
Vector Backbone Description: Backbone Size:6273; Vector Backbone:psiCHECK-2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_67632 Copy
Species: Mus musculus
Genetic Insert: MBII85 snoRNA
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_67631 Copy
Species: Mus musculus
Genetic Insert: MBII52 snoRNA
Vector Backbone Description: Vector Backbone:pCR4-TOPO; Vector Types:for in vitro transcription; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_67647 Copy
Species: Mus musculus
Genetic Insert: MBII85 snoRNA
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_67645 Copy
Species: Mus musculus
Genetic Insert: MBII85 snoRNA
Vector Backbone Description: Vector Backbone:pCRII-TOPO; Vector Types:for in vitro transcription; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_67646 Copy
Species: Mus musculus
Genetic Insert: MBII85 snoRNA
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_67643 Copy
Species: Mus musculus
Genetic Insert: ZFP809
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_67788 Copy
Species: Mus musculus
Genetic Insert: EKLF
Vector Backbone Description: Backbone Marker:GE Healthcare Life Sciences; Backbone Size:4969; Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Mutations G299S, and T305S have no functional consequences.
Proper citation: RRID:Addgene_67836 Copy
Species: Mus musculus
Genetic Insert: EKLF
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4076; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Mutations G299S, T305S have no functional consequences.
Proper citation: RRID:Addgene_67837 Copy
Species: Mus musculus
Genetic Insert: EKLF
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4076; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Entire EKLF open reading frame is defined as nucleotides 71-1215 and described in PMID 7682653. Mutations G299S and T305S have no functional consequences.
Proper citation: RRID:Addgene_67833 Copy
Species: Mus musculus
Genetic Insert: AAV-hsyn-flexed-dsRed-miR30-vgat2.shRNA
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC; Vector Types:Mammalian Expression, Mouse Targeting, AAV, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
References:
Comments: this plasmid is modified from the pRIME system. See:
Stegmeier, F., Hu, G., Rickles, R.J., Hannon, G.J., and Elledge, S.J. (2005). A
lentiviral microRNA-based system for single-copy polymerase II-regulated
RNA interference in mammalian cells. Proc. Natl. Acad. Sci. USA 102,
13212–13217
We used The pPRIME-CMV-dsRed-FF3 vector, a gift from Stephen Elledge (Addgene plasmid 11664), as the building block to make the AAV-hsyn-flex-dsRed-miR30-vgat2.shRNA plasmid.
transgene expression is Cre-dependent; contains flex switch
All the enzymes are unique cutters, except EcoRI and XhoI, the insert dsRed-shRNA can be retrieved using AscI and NheI, giving 1.2kb and 4.8kb bands.
The insert was read using primers in the dsred sequence:
dsred 410: CGTAATGCAGAAGAAGACTATG
dsred 530R: CCATGTAGATGGACTTGAACTC
Proper citation: RRID:Addgene_67845 Copy
Species: Mus musculus
Genetic Insert: Cav3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:peGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_67886 Copy
Species: Mus musculus
Genetic Insert: Vinculin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_67935 Copy
Species: Mus musculus
Genetic Insert: YC3.60
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4000; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_67899 Copy
Species: Mus musculus
Genetic Insert: nesprin-2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcdna 3.1 -; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_68128 Copy
Species: Mus musculus
Genetic Insert: mouse nesprin mini 2G
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcdna 3.1 +; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_68127 Copy
Species: Mus musculus
Genetic Insert: Semaphorin-4A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_68029 Copy
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