Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Species: Pseudomonas aeruginosa
Genetic Insert: PilB
Vector Backbone Description: Backbone Size:3643; Vector Backbone:pUCP20; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Amber mutation was inserted concomitant to replacing the original UAG stop codon with UAA from pUCP20 PilB
Proper citation: RRID:Addgene_162852 Copy
Species: Pseudomonas aeruginosa
Genetic Insert: PilB
Vector Backbone Description: Backbone Size:3643; Vector Backbone:pUCP20; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Amber mutation was inserted concomitant to replacing the original UAG stop codon with UAA from pUCP20 PilB
Proper citation: RRID:Addgene_162855 Copy
Species: Pseudomonas aeruginosa
Genetic Insert: PilB
Vector Backbone Description: Backbone Size:3643; Vector Backbone:pUCP20; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Amber mutation was inserted concomitant to replacing the original UAG stop codon with UAA from pUCP20 PilB
Proper citation: RRID:Addgene_162856 Copy
Species: Pseudomonas aeruginosa
Genetic Insert: PilB
Vector Backbone Description: Backbone Size:3643; Vector Backbone:pUCP20; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Amber mutation was inserted concomitant to replacing the original UAG stop codon with UAA from pUCP20 PilB
Proper citation: RRID:Addgene_162859 Copy
Species: Pseudomonas aeruginosa
Genetic Insert: PilB
Vector Backbone Description: Backbone Size:3643; Vector Backbone:pUCP20; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Amber mutation was inserted concomitant to replacing the original UAG stop codon with UAA from pUCP20 PilB
Proper citation: RRID:Addgene_162858 Copy
Species: Pseudomonas aeruginosa
Genetic Insert: PilB
Vector Backbone Description: Backbone Size:3643; Vector Backbone:pUCP20; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Amber mutation was inserted concomitant to replacing the original UAG stop codon with UAA from pUCP20 PilB
Proper citation: RRID:Addgene_162849 Copy
Species: Pseudomonas aeruginosa
Genetic Insert: PilB
Vector Backbone Description: Backbone Size:3643; Vector Backbone:pUCP20; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Amber mutation was inserted concomitant to replacing the original UAG stop codon with UAA from pUCP20 PilB
Proper citation: RRID:Addgene_162846 Copy
Species: Pseudomonas aeruginosa
Genetic Insert: PilB
Vector Backbone Description: Backbone Size:3643; Vector Backbone:pUCP20; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: This gene was PCR amplified from PAK chromosome and a histag was added to the C-terminal end. The backbone was also amplifed into two fragments from promoter or terminator region to resistance marker.
Proper citation: RRID:Addgene_162845 Copy
Species: Pseudomonas aeruginosa
Genetic Insert: PilB
Vector Backbone Description: Backbone Size:3643; Vector Backbone:pUCP20; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Amber mutation was inserted concomitant to replacing the original UAG stop codon with UAA from pUCP20 PilB
Proper citation: RRID:Addgene_162847 Copy
Species: Synthetic
Genetic Insert: Puromycin resistant; mCherry linked to StrepTagII-ProtC-FLAG
Vector Backbone Description: Backbone Marker:Vectorbuilder; Vector Backbone:pLV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36240778
Comments: #12 out of 20 mCherry tagged combinations . Please visit https://doi.org/10.1101/2021.06.29.449991 for bioRxiv preprint.
Proper citation: RRID:Addgene_162110 Copy
Species: Synthetic
Genetic Insert: Puromycin resistant; mCherry linked to StrepTagII-HA-FLAG
Vector Backbone Description: Backbone Marker:Vectorbuilder; Vector Backbone:pLV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36240778
Comments: #14 out of 20 mCherry tagged combinations . Please visit https://doi.org/10.1101/2021.06.29.449991 for bioRxiv preprint.
Proper citation: RRID:Addgene_162112 Copy
Species: Synthetic
Genetic Insert: Puromycin resistant; mCherry linked to StrepTagII-FLAG-AU1
Vector Backbone Description: Backbone Marker:Vectorbuilder; Vector Backbone:pLV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36240778
Comments: #16 out of 20 mCherry tagged combinations . Please visit https://doi.org/10.1101/2021.06.29.449991 for bioRxiv preprint.
Proper citation: RRID:Addgene_162114 Copy
Species: Pseudomonas aeruginosa
Genetic Insert: phzSM
Vector Backbone Description: Backbone Marker:iGEM; Backbone Size:3339; Vector Backbone:pSB4A3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_165620 Copy
Species: Synthetic
Genetic Insert: Puromycin resistant; mCherry linked to StrepTagII-HA-AU1
Vector Backbone Description: Backbone Marker:Vectorbuilder; Vector Backbone:pLV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36240778
Comments: #15 out of 20 mCherry tagged combinations . Please visit https://doi.org/10.1101/2021.06.29.449991 for bioRxiv preprint.
Proper citation: RRID:Addgene_162113 Copy
Species: E. coli
Genetic Insert: LetAB
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3174; Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35077766
Proper citation: RRID:Addgene_170410 Copy
Species: Synthetic
Genetic Insert: Puromycin resistant; mCherry linked to ProtC-HA-FLAG
Vector Backbone Description: Backbone Marker:Vectorbuilder; Vector Backbone:pLV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36240778
Comments: #17 out of 20 mCherry tagged combinations . Please visit https://doi.org/10.1101/2021.06.29.449991 for bioRxiv preprint.
Proper citation: RRID:Addgene_162115 Copy
Species: E. coli
Genetic Insert: LetAB
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:4571; Vector Backbone:pBAD-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35077766
Proper citation: RRID:Addgene_170404 Copy
Species: E. coli
Genetic Insert: LetAB
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:4571; Vector Backbone:pBAD-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35077766
Proper citation: RRID:Addgene_170402 Copy
Species: Pseudomonas aeruginosa
Genetic Insert: PilB
Vector Backbone Description: Backbone Size:3643; Vector Backbone:pUCP20; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Amber mutation was inserted concomitant to replacing the original UAG stop codon with UAA from pUCP20 PilB
Proper citation: RRID:Addgene_162862 Copy
Species: Pseudomonas aeruginosa
Genetic Insert: PilB
Vector Backbone Description: Backbone Size:3643; Vector Backbone:pUCP20; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Amber mutation was inserted concomitant to replacing the original UAG stop codon with UAA from pUCP20 PilB
Proper citation: RRID:Addgene_162861 Copy
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the NIF Resources search. From here you can search through a compilation of resources used by NIF and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that NIF has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on NIF then you can log in from here to get additional features in NIF such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into NIF you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within NIF that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.