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Species: Homo sapiens
Genetic Insert: chimeric TRF
Vector Backbone Description: Vector Backbone:pBabe; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: TRF chimera with iDDR region of TRF2 (aa 407-431 compared to GenBank accession number NP_005643.1 - PKGKWNSSNGVEEKETWVEEDELFQ) replacing aa 346-364 of TRF1 (NP_059523.2).
Proper citation: RRID:Addgene_44576 Copy
Species: Homo sapiens
Genetic Insert: KRAS 3'UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4281; Vector Backbone:pGL4.75; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: A substitute for pGL3-KRAS LCS6m in Chin et al., 2008.
Proper citation: RRID:Addgene_44571 Copy
Species: Homo sapiens
Genetic Insert: Kif17
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4470; Vector Backbone:pDONR 201; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
References:
Comments: Kinesin 2 family protein KIF17, the construct contain the motor, neck and first coil-coil structure [1- 490 amino acids] of the protein.
Proper citation: RRID:Addgene_44706 Copy
Species: Homo sapiens
Genetic Insert: Rab3A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4470; Vector Backbone:pDONR 201; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_44698 Copy
Species: Homo sapiens
Genetic Insert: Rab5A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4470; Vector Backbone:pDONR 201; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_44699 Copy
Species: Homo sapiens
Genetic Insert: Maternally Expressed Gene 3
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_44732 Copy
Species: Homo sapiens
Genetic Insert: Maternally Expressed Gene 3
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_44727 Copy
Species: Homo sapiens
Genetic Insert: SC35
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1/myc-His (-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Both tags are in frame with the insert.
Proper citation: RRID:Addgene_44721 Copy
Species: Homo sapiens
Genetic Insert: Lacritin
Vector Backbone Description: Backbone Marker:New England Biolabs, INC; Backbone Size:7477; Vector Backbone:pTYB1; Vector Types:Bacterial Expression, Protein Expression Vector; Bacterial Resistance:Ampicillin
References:
Comments: Please note that Addgene's sequencing results identified a few differences in the vector backbone when compared to the full plasmid sequence. The depositing laboratory indicates that these differences are not a concern for the function of the plasmid.
Proper citation: RRID:Addgene_45072 Copy
Species: Homo sapiens
Genetic Insert: CENP-T (aa1-374) Laci-NLS
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pIC242; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_45109 Copy
Species: Homo sapiens
Genetic Insert: TfR
Vector Backbone Description: Backbone Size:6755; Vector Backbone:pBa-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination.
XL 10-Gold, Stbl3, OmniMax2, DH5alpha, and XL 1-Blue are suitable growth strains.
Proper citation: RRID:Addgene_45060 Copy
Species: Homo sapiens
Genetic Insert: ZNF668 Deletion11
Vector Backbone Description: Backbone Marker:sigma; Backbone Size:6400; Vector Backbone:p3XFLAG-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_45040 Copy
Species: Homo sapiens
Genetic Insert: ZNF668
Vector Backbone Description: Backbone Size:4968; Vector Backbone:pGEX-4T-3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_45042 Copy
Species: Homo sapiens
Genetic Insert: ZNF668 Deletion5
Vector Backbone Description: Backbone Marker:sigma; Backbone Size:6400; Vector Backbone:p3XFLAG-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_45034 Copy
Species: Homo sapiens
Genetic Insert: ZNF668 Deletion8
Vector Backbone Description: Backbone Marker:sigma; Backbone Size:6400; Vector Backbone:p3XFLAG-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_45037 Copy
Species: Homo sapiens
Genetic Insert: ZNF668 Deletion7
Vector Backbone Description: Backbone Marker:sigma; Backbone Size:6400; Vector Backbone:p3XFLAG-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_45036 Copy
Species: Homo sapiens
Genetic Insert: FUS (Full length)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_44985 Copy
Species: Homo sapiens
Genetic Insert: ZNF668 Deletion3
Vector Backbone Description: Backbone Marker:sigma; Backbone Size:6400; Vector Backbone:p3XFLAG-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_45032 Copy
Species: Homo sapiens
Genetic Insert: FUS (Full length)
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_44978 Copy
Species: Homo sapiens
Genetic Insert: H2B-GFP-UVR8(2x)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5308; Vector Backbone:pIRESneo2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_44970 Copy
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