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Species: Mus musculus
Genetic Insert: Klf4
Vector Backbone Description: Backbone Size:9941; Vector Backbone:FUdeltaGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18371448
Comments: From article: A tet-inducible lentivirus called LV-tetO was generated by removing the EcoRI/PacI fragment containing the ubiquitin promoter elements from the FUdeltaGW vector (modified from FUGW by removal of GFP with EcoRI-XbaI and re-creation of the EcoRI site) and replacing it with tetO sequences derived from pTet-Splice by EcoRI/XhoI digestion.cDNAs for c-Myc (T58A mutant), Klf4, Oct4, and Sox2 were isolated from pMIG or pMX vectors and cloned into LV-tetO using a unique EcoRI restriction site.
Proper citation: RRID:Addgene_19764 Copy
Species: Homo sapiens
Genetic Insert: c-myc
Vector Backbone Description: Backbone Size:9941; Vector Backbone:FUdeltaGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18371448
Comments: From article: A tet-inducible lentivirus called LV-tetO was generated by removing the EcoRI/PacI fragment containing the ubiquitin promoter elements from the FUdeltaGW vector (modified from FUGW by removal of GFP with EcoRI-XbaI and re-creation of the EcoRI site) and replacing it with tetO sequences derived from pTet-Splice by EcoRI/XhoI digestion.cDNAs for c-Myc (T58A mutant), Klf4, Oct4, and Sox2 were isolated from pMIG or pMX vectors and cloned into LV-tetO using a unique EcoRI restriction site.
Proper citation: RRID:Addgene_19763 Copy
Species: Mus musculus
Genetic Insert: Oct4
Vector Backbone Description: Backbone Size:9941; Vector Backbone:FUdeltaGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18371448
Comments: From article: A tet-inducible lentivirus called LV-tetO was generated by removing the EcoRI/PacI fragment containing the ubiquitin promoter elements from the FUdeltaGW vector (modified from FUGW by removal of GFP with EcoRI-XbaI and re-creation of the EcoRI site) and replacing it with tetO sequences derived from pTet-Splice by EcoRI/XhoI digestion.cDNAs for c-Myc (T58A mutant), Klf4, Oct4, and Sox2 were isolated from pMIG or pMX vectors and cloned into LV-tetO using a unique EcoRI restriction site.
Note that the region immediately upstream of the insert in the author's sequence is not 100% accurate. See Addgene's quality control sequence for this region.
Proper citation: RRID:Addgene_19766 Copy
Species: Mus musculus
Genetic Insert: Sox2
Vector Backbone Description: Backbone Size:9941; Vector Backbone:FUdeltaGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18371448
Comments: From article: A tet-inducible lentivirus called LV-tetO was generated by removing the EcoRI/PacI fragment containing the ubiquitin promoter elements from the FUdeltaGW vector (modified from FUGW by removal of GFP with EcoRI-XbaI and re-creation of the EcoRI site) and replacing it with tetO sequences derived from pTet-Splice by EcoRI/XhoI digestion.cDNAs for c-Myc (T58A mutant), Klf4, Oct4, and Sox2 were isolated from pMIG or pMX vectors and cloned into LV-tetO using a unique EcoRI restriction site.
Proper citation: RRID:Addgene_19765 Copy
Genetic Insert: scrambled shRNA
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pEGFP-U6; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18840295
Comments: The scrambled sequence for shR-Scr - 5'-GCGATGCTCTAAGGTTCTATCAA
Proper citation: RRID:Addgene_19800 Copy
Genetic Insert: shRNA
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pEGFP-U6; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18840295
Comments: Target sequence for shR-E1-5 - 5'-GATGATGCTCCGGTAACTGTTTC
Proper citation: RRID:Addgene_19805 Copy
Genetic Insert: shRNA
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pEGFP-U6; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18840295
Comments: Target sequence for shR-E1-4 - 5'-GCACAACCTAACGTCGACAAACT
Proper citation: RRID:Addgene_19804 Copy
Genetic Insert: shRNA
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pEGFP-U6; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18840295
Comments: Target sequence for shR-E1-7 - 5'-GCAATTAGGACGTTTCAACAGAT
Proper citation: RRID:Addgene_19807 Copy
Genetic Insert: shRNA
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pEGFP-U6; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18840295
Comments: Target sequence for shR-E1-6 - 5'-GATCTGGTGTGTTATCGACGAAA
Proper citation: RRID:Addgene_19806 Copy
Species: Homo sapiens
Genetic Insert: MKL1
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pCMV-3xFLAG-7.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18694962
Comments: The 3xFlag in the vector is from Sigma, p3xFlag-CMV-7.1 has variant flag sequences (DYKDHD) and is recognized well by their anti-flag monoclonal M2.
Proper citation: RRID:Addgene_19852 Copy
Species: Homo sapiens
Genetic Insert: RHEBL1 3'UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6249; Vector Backbone:psiCHECK-2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18978028
Comments: There are small differences between author's sequence and Addgene sequence. Depositor is aware of the changes. These changes in the sequence do not affect the function of the construct.
Proper citation: RRID:Addgene_19858 Copy
Species: Homo sapiens
Genetic Insert: GSC 3'UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6249; Vector Backbone:psiCHECK-2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18978028
Proper citation: RRID:Addgene_19859 Copy
Species: Mus musculus
Genetic Insert: RASSF1A
Vector Backbone Description: Backbone Size:4657; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11857081
Proper citation: RRID:Addgene_1980 Copy
Species: Homo sapiens
Genetic Insert: SIRT4 3' UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6249; Vector Backbone:psiCHECK-2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18978028
Proper citation: RRID:Addgene_19860 Copy
Species: Homo sapiens
Genetic Insert: IRF9 3'UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6249; Vector Backbone:psiCHECK-2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18978028
Comments: There are small differences between author's sequence and Addgene sequence. Depositor is aware of the changes. These changes in the sequence do not affect the function of the construct.
Proper citation: RRID:Addgene_19863 Copy
Species: Homo sapiens
Genetic Insert: hRpt6
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7700; Vector Backbone:pQCXIP; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18922472
Proper citation: RRID:Addgene_19793 Copy
Species: Homo sapiens
Genetic Insert: NFRKB
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5400; Vector Backbone:BacPAK8; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18922472
Proper citation: RRID:Addgene_19792 Copy
Species: Homo sapiens
Genetic Insert: CCDC95
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18922472
Proper citation: RRID:Addgene_19795 Copy
Species: Homo sapiens
Genetic Insert: TRF2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2820; Vector Backbone:pENTR/D-TOPO; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9476899
Comments: The missing codon is due to a reverse primer with a stop codon at 1474 of TRF2 gene: GAATTC TTA CTTCTGCTTTTTTGTTATATTGGTTGT. It generates a 50aa in frame deletion from c-terminal tail.
Proper citation: RRID:Addgene_19797 Copy
Species: Homo sapiens
Genetic Insert: Arp8
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18922472
Proper citation: RRID:Addgene_19796 Copy
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