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Species: Homo sapiens
Genetic Insert: RGS7
Vector Backbone Description: Backbone Size:6583; Vector Backbone:PCDF1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29330521
Proper citation: RRID:Addgene_113730 Copy
Species: Synthetic
Genetic Insert: SaCas9 gRNA Cassete
Vector Backbone Description: Backbone Marker:Alligent Technologies; Vector Backbone:AAV; Vector Types:AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30483052
Proper citation: RRID:Addgene_113696 Copy
Species: Other
Genetic Insert: PpU6P::sgRNA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2538; Vector Backbone:pDONR221-P1P2; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Proper citation: RRID:Addgene_113735 Copy
Species: Other
Genetic Insert: PpU6P::sgRNA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2604; Vector Backbone:pDONR221-P1P5r; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Proper citation: RRID:Addgene_113737 Copy
Species: Other
Genetic Insert: H2B-sfGFP-T2A-tdTomato
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_113841 Copy
Species: Other
Genetic Insert: H2B-sfGFP-T2A-tdTomato
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_113840 Copy
Species: Other
Genetic Insert: sfGFP-T2A-tdTomato
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_113846 Copy
Species: Other
Genetic Insert: pac-T2A-iCre
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please note that iCre in this plasmid has an extra Valine upstream of the SV40 nuclear localization signal that does not affect the function. We have also deposited a version of this plasmid without the extra Valine, plasmid #125821.
Proper citation: RRID:Addgene_113849 Copy
Species: Other
Genetic Insert: H2B-sfGFP-P2A-tdTomato
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please read a Figshare page (https://doi.org/10.6084/m9.figshare.13237328.v16) before ordering this plasmid. For unknown reasons, the P2A in this construct doesn't skip very well. The T2A versions do skip well (e.g., https://www.addgene.org/113839/).
Proper citation: RRID:Addgene_113847 Copy
Species: Homo sapiens
Genetic Insert: ZNF423
Vector Backbone Description: Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pZNF423-donor
Proper citation: RRID:Addgene_113790 Copy
Species: Homo sapiens
Genetic Insert: MSX2
Vector Backbone Description: Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pMSX2-donor
Proper citation: RRID:Addgene_113793 Copy
Species: Homo sapiens
Genetic Insert: TFAP2C
Vector Backbone Description: Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pTFAP2C-donor
Proper citation: RRID:Addgene_113792 Copy
Species: Homo sapiens
Genetic Insert: TCF15
Vector Backbone Description: Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pTCF15-donor
Proper citation: RRID:Addgene_113798 Copy
Species: Homo sapiens
Genetic Insert: TCF15 homology arms flanking EGFP-IRES-Neo cassette
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Cloning source: K562.
Use with plasmid pTCF15.1.0-gDNA
Proper citation: RRID:Addgene_113830 Copy
Species: Homo sapiens
Genetic Insert: SPDEF
Vector Backbone Description: Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pSPDEF-donor
Proper citation: RRID:Addgene_113796 Copy
Species: Homo sapiens
Genetic Insert: HOXC13
Vector Backbone Description: Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pHOXC13-donor
Proper citation: RRID:Addgene_113795 Copy
Species: Homo sapiens
Genetic Insert: DEAF1
Vector Backbone Description: Backbone Marker:Zhang lab (Addgene plasmid ID: 62987); Vector Backbone:pX462; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. This is a nickase guide and must be used with the additional nickase plasmid pDEAF1.2.1-gDNA and donor plasmid pDEAF1-donor.
Proper citation: RRID:Addgene_113799 Copy
Species: Synthetic
Genetic Insert: osTIR1-GFP
Vector Backbone Description: Vector Backbone:pbluescript; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29889212
Comments: This vector is good for targeting experiment in early embryos and mouse cell lines. Viability problem have been observed when generating knock-in mice.
Proper citation: RRID:Addgene_113832 Copy
Species: Other
Genetic Insert: H2B-sfGFP-T2A-tdTomato
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_113839 Copy
Species: Other
Genetic Insert: mTagBFP2-T2A-iCre
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please note that iCre in this plasmid has an extra Valine upstream of the SV40 nuclear localization signal that does not affect the function. We have also deposited a version of this plasmid without the extra Valine, plasmid #125822.
Proper citation: RRID:Addgene_113837 Copy
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