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Species: Homo sapiens
Genetic Insert: RepoMan
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_44213 Copy
Species: Homo sapiens
Genetic Insert: TAF5
Vector Backbone Description: Vector Backbone:pMX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_44336 Copy
Species: Homo sapiens
Genetic Insert: human GPR56 C-terminal fragment with signal peptide
Vector Backbone Description: Backbone Marker:unknown; Backbone Size:7500; Vector Backbone:Barcode; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_44201 Copy
Species: Homo sapiens
Genetic Insert: mitoDsRed
Vector Backbone Description: Backbone Size:6727; Vector Backbone:pLV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: Limitations of allotopic expression of mitochondrial genes in mammalian cells. Oca-Cossio J, Kenyon L, Hao H, Moraes CT.
Genetics. 2003 Oct;165(2):707-20. PMID: 14573482
Proper citation: RRID:Addgene_44386 Copy
Species: Homo sapiens
Genetic Insert: mitoGFP
Vector Backbone Description: Backbone Size:6727; Vector Backbone:pLV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_44385 Copy
Species: Homo sapiens
Genetic Insert: TOM1-VHS (1-152)
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGST1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_44423 Copy
Species: Homo sapiens
Genetic Insert: HRS-VHS (1-143)
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGST1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_44416 Copy
Species: Homo sapiens
Genetic Insert: GGA2-VHS
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGST1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_44419 Copy
Species: Homo sapiens
Genetic Insert: STAM1-VHS
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGST2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_44414 Copy
Species: Homo sapiens
Genetic Insert: hM4D(Gi)-mCherry
Vector Backbone Description: Backbone Size:4818; Vector Backbone:pAAV; Vector Types:AAV, Adeno Associated Viral Vector; Bacterial Resistance:Ampicillin
References:
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_44362 Copy
Species: Homo sapiens
Genetic Insert: TFEB
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_44446 Copy
Species: Homo sapiens
Genetic Insert: MAVS-Fis1 chimeric protein
Vector Backbone Description: Backbone Size:6600; Vector Backbone:pMSCV2.2 IRES GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_44556 Copy
Species: Homo sapiens
Genetic Insert: v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC)
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_44652 Copy
Species: Homo sapiens
Genetic Insert: v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC)
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_44654 Copy
Species: Homo sapiens
Genetic Insert: EF1a-GFP:2A:CHER(HA)
Vector Backbone Description: Backbone Marker:LJ Chang; Backbone Size:8680; Vector Backbone:pTY; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: GFP-2A-mCherH was created using a three-step PCR strategy described by Symczak et al [46] that included the addition of a GSG linker sequence upstream of the PTV1 2A peptide sequence.
mCherry was tagged with HA to enable detection using HA antibody.
Proper citation: RRID:Addgene_44597 Copy
Species: Homo sapiens
Genetic Insert: K8
Vector Backbone Description: Vector Backbone:pCIN4-Puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note that Addgene's sequencing results identified two silent mutations in K8 when compared to GenBank accession NM_002273.3. The protein sequence is not affected by these differences.
Proper citation: RRID:Addgene_44626 Copy
Species: Homo sapiens
Genetic Insert: K8
Vector Backbone Description: Vector Backbone:pECE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_44621 Copy
Species: Homo sapiens
Genetic Insert: K18
Vector Backbone Description: Vector Backbone:pGEM-1; Vector Types:Cloning; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_44623 Copy
Species: Homo sapiens
Genetic Insert: chimeric TRF
Vector Backbone Description: Vector Backbone:pBabe; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: TRF2 construct in which the DNA-binding domain (aa444-500 when compared to NP_005643.1) was replaced with the TRF1 MYB domain (aa337-439 when compared to NP_059523.2).
According to the depositing laboratory, the deletion of A434 does not affect TRF2 function.
Proper citation: RRID:Addgene_44575 Copy
Species: Homo sapiens
Genetic Insert: chimeric TRF
Vector Backbone Description: Vector Backbone:pBabe; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: TRF2 construct in which the Hinge domain (aa254-443 when compared to NP_005643.1) was replaced with Hinge domain of TRF1 (aa265-376 when compared to NP_059523.2)
Proper citation: RRID:Addgene_44574 Copy
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