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  • Organism:homo sapiens (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pGEX6P1-HA-MEK1 C121S
 
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RRID:Addgene_53164 MEK1 Homo sapiens Ampicillin PMID:24717435 Backbone Marker:GE Healthcare; Backbone Size:4984; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin C121S 2026-07-25 12:48:27 0
pTRCHisB-TRF2∆B
 
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1+ mentions
RRID:Addgene_53208 TRF2 Delta Basic Homo sapiens Ampicillin PMID:25115914 Please note that the correct TERF2 reference sequence for this plasmid is Q15554.2 (not the more recent Q15554.3 version). Backbone Marker:Invitrogen; Backbone Size:4404; Vector Backbone:pTrcHisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin contains amino acids 47-500 2026-07-25 12:48:27 1
pTrcHisB-TRF1
 
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1+ mentions
RRID:Addgene_53209 TRF1 Homo sapiens Ampicillin PMID:25115914 Backbone Marker:Invitrogen; Backbone Size:4404; Vector Backbone:pTrcHisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 3-439 2026-07-25 12:48:27 3
pWZLblasti-HA-ERK2 R67S
 
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1+ mentions
RRID:Addgene_53172 ERK2 Homo sapiens Ampicillin PMID:24717435 Backbone Size:5100; Vector Backbone:pWZLblasti; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin R67S 2026-07-25 12:48:27 1
pWZLblasti-HA-ERK2 D321N
 
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RRID:Addgene_53173 ERK2 Homo sapiens Ampicillin PMID:24717435 The sequence discrepancies found during the QC process have no functional consequence. Backbone Size:5100; Vector Backbone:pWZLblasti; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin D321N 2026-07-25 12:48:27 0
pGEX6P1-HA-ERK5 K84R
 
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1+ mentions
RRID:Addgene_53171 ERK5 Homo sapiens Ampicillin PMID:24717435 Backbone Marker:GE Healthcare; Backbone Size:4984; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin K84R 2026-07-25 12:48:27 1
pBABEpuro-HA-MEK1-MEK5DD
 
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RRID:Addgene_53176 MEK1 (1-201)-MEK5 (498–1344) fusion Homo sapiens Ampicillin PMID:24717435 The sequence discrepancies found during the QC process have no functional consequence. Backbone Size:5169; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin MEK1 (1-201) MEK5(498–1344 with S311D/T315D) 2026-07-25 12:48:27 0
pWZLblasti-HA-MEK1-MEK5DD
 
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RRID:Addgene_53177 MEK1 (1-201)-MEK5 (498–1344) fusion Homo sapiens Ampicillin PMID:24717435 The sequence discrepancies found during the QC process have no functional consequence. Backbone Size:5100; Vector Backbone:pWZLblasti; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin MEK1 (1-201) MEK5(498–1344 with S311D/T315D) 2026-07-25 12:48:27 0
pWZLblasti-HA-ERK2 GOF
 
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1+ mentions
RRID:Addgene_53174 ERK2 Homo sapiens Ampicillin PMID:24717435 The sequence discrepancies found during the QC process have no functional consequence. Backbone Size:5100; Vector Backbone:pWZLblasti; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin R67S/D321N 2026-07-25 12:48:27 1
PCRT7 tetR pLtetO MBP-MEK1 XX Amp
 
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1+ mentions
RRID:Addgene_53225 MEK1 Homo sapiens Ampicillin Backbone Marker:Rinehart lab, Addgene plasmid 52053; Vector Backbone:pCRT7; Vector Types:Bacterial Expression, phosphoprotein synthesis; Bacterial Resistance:Ampicillin Codon optimized for E.coli, both codons for Ser218 and Ser222 were changed to amber codons 2026-07-25 12:48:27 1
SK-hPar3
 
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RRID:Addgene_53229 Par3 Homo sapiens Ampicillin Backbone Marker:Stratagene; Backbone Size:2958; Vector Backbone:pBluescript SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:48:27 0
pBABEpuro-HA-MEK1
 
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1+ mentions
RRID:Addgene_53195 MEK1 Homo sapiens Ampicillin PMID:24717435 Backbone Size:5169; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-07-25 12:48:27 3
pBJ-FLAG-hPar4
 
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1+ mentions
RRID:Addgene_53231 Par4 Homo sapiens Ampicillin Backbone Marker:Mark Davis lab; Vector Backbone:pBJ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Deletion of aa1-24 (native signal peptide) 2026-07-25 12:48:27 1
pBABEpuro-HA-MEK5 CBM-E
 
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RRID:Addgene_53199 MEK5 Homo sapiens Ampicillin PMID:24717435 The sequence discrepancies found during the QC process have no functional consequence. Backbone Size:5169; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin L280A/H281A/M323A/Q332A 2026-07-25 12:48:27 0
pBABEpuro-HA-MEK1 CBM
 
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RRID:Addgene_53196 MEK1 Homo sapiens Ampicillin PMID:24717435 The N12D mutation found during the QC process has no functional consequence. Backbone Size:5169; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin M187A/H188A/M230A/H239A 2026-07-25 12:48:27 0
pBABEpuro-HA-MEK1 C121S
 
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RRID:Addgene_53197 MEK1 Homo sapiens Ampicillin PMID:24717435 The S241P mutation found during the QC process has no functional consequence. This plasmid is prone to recombination. We recommend screening 2-3 colonies to ensure that the full plasmid is selected. Backbone Size:5169; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin C121S 2026-07-25 12:48:27 0
pMyc4ElbLuc.
 
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1+ mentions
RRID:Addgene_53246 pMyc4ElbLuc Homo sapiens Ampicillin PMID:8386367 Vector Backbone:pG5ElbLuc; Vector Types:; Bacterial Resistance:Ampicillin To prepare plasmids with one, two, three, and four Myc binding sites (underlined), a 26-mer double-stranded oligonucleotide (5'-AGCTTAACTGACCACGTGGTCAACTA- 3' and 5'-AGCTTAGTTGACCACGTGGTCAGTTA- 3') was employed in a ligation reaction to obtain the plasmids pMyc4ElbLuc, 2026-07-25 12:48:27 2
pMyc2ElbLuc,
 
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RRID:Addgene_53244 pMyc2ElbLuc Homo sapiens Ampicillin PMID:8386367 Vector Backbone:pG5ElbLuc; Vector Types:; Bacterial Resistance:Ampicillin To prepare plasmids with one, two, three, and four Myc binding sites (underlined), a 26-mer double-stranded oligonucleotide (5'-AGCTTAACTGACCACGTGGTCAACTA- 3' and 5'-AGCTTAGTTGACCACGTGGTCAGTTA- 3') was employed in a ligation reaction to obtain the plasmids pMyc2ElbLuc, 2026-07-25 12:48:27 0
pMyc3ElbLuc
 
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RRID:Addgene_53245 pMyc3ElbLuc Homo sapiens Ampicillin PMID:8386367 Vector Backbone:pG5ElbLuc; Vector Types:; Bacterial Resistance:Ampicillin To prepare plasmids with one, two, three, and four Myc binding sites (underlined), a 26-mer double-stranded oligonucleotide (5'-AGCTTAACTGACCACGTGGTCAACTA- 3' and 5'-AGCTTAGTTGACCACGTGGTCAGTTA- 3') was employed in a ligation reaction to obtain the plasmids pMyc3ElbLuc, 2026-07-25 12:48:27 0
pMyc0ElbLuc
 
Resource Report
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RRID:Addgene_53242 pMycOElbLuc Homo sapiens Ampicillin PMID:8386367 Vector Backbone:pG5ElbLuc; Vector Types:; Bacterial Resistance:Ampicillin 2026-07-25 12:48:27 0

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