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Species: Homo sapiens
Genetic Insert: hnRNPF
Vector Backbone Description: Vector Backbone:pTHMT; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33349959
Proper citation: RRID:Addgene_139114 Copy
Species: Homo sapiens
Genetic Insert: hnRNPA2
Vector Backbone Description: Vector Backbone:pJ411; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33349959
Proper citation: RRID:Addgene_139118 Copy
Species: Homo sapiens
Genetic Insert: hnRNPA2
Vector Backbone Description: Vector Backbone:pJ411; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33349959
Proper citation: RRID:Addgene_139122 Copy
Species: Homo sapiens
Genetic Insert: hnRNPA2
Vector Backbone Description: Vector Backbone:pJ411; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33349959
Proper citation: RRID:Addgene_139123 Copy
Species: Homo sapiens
Genetic Insert: hnRNPA2
Vector Backbone Description: Vector Backbone:pJ411; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33349959
Proper citation: RRID:Addgene_139120 Copy
Species: Homo sapiens
Genetic Insert: FUS
Vector Backbone Description: Vector Backbone:pJ411; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33349959
Proper citation: RRID:Addgene_139126 Copy
Species: Homo sapiens
Genetic Insert: FUS
Vector Backbone Description: Vector Backbone:pJ411; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33349959
Proper citation: RRID:Addgene_139127 Copy
Species: Homo sapiens
Genetic Insert: hnRNPA2
Vector Backbone Description: Vector Backbone:pJ411; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33349959
Proper citation: RRID:Addgene_139125 Copy
Species: Homo sapiens
Genetic Insert: iRFP:MTOR(2021-2113):[GGSA]2:GOLGB1(3097-3226)
Vector Backbone Description: Vector Backbone:piRFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32211893
Proper citation: RRID:Addgene_139313 Copy
Species: Homo sapiens
Genetic Insert: AO7 aa 126-258
Vector Backbone Description: Backbone Marker:Millipore-Sigma; Backbone Size:5420; Vector Backbone:pETDUET-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26475854
Proper citation: RRID:Addgene_139319 Copy
Species: Homo sapiens
Genetic Insert: ID1
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:p15A-PB; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Kanamycin
Comments: Genomic sequence subcloned by recombineering from a clone of RPCI-11 human genomic DNA library. The exact DNA sequence in this plasmid differs somewhat from the reference human genome sequence because the reference sequence is a composite sequence, i.e., the reference ID1 region sequence may not be derived from the RPCI-11 BAC clone utilized for this construct.
The ID1 intron was placed after G198, i.e., after about a third of the sequence encoding the active site of the cre protein. See https://doi.org/10.1073/pnas.2011448117 and https://doi.org/10.1093%2Fnar%2Fgkq384. Thus, without splicing, no catalytically active cre protein sequence is expected to be translated from this sequence.
Please make sure the plasmid DNA preparation is pure by gel electrophoresis before using.
Proper citation: RRID:Addgene_139280 Copy
Species: Homo sapiens
Genetic Insert: sgRNA to insert BRCA2 V572I using base editing
Vector Backbone Description: Backbone Size:2633; Vector Backbone:B52 (Addgene #100708); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32160537
Proper citation: RRID:Addgene_139321 Copy
Species: Homo sapiens
Genetic Insert: sgRNA to insert BRCA2 S3070F using base editing
Vector Backbone Description: Backbone Size:2633; Vector Backbone:B52 (Addgene #100708); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32160537
Proper citation: RRID:Addgene_139325 Copy
Species: Homo sapiens
Genetic Insert: sgRNA to insert BRCA2 V2102I using base editing
Vector Backbone Description: Backbone Size:2633; Vector Backbone:B52 (Addgene #100708); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32160537
Proper citation: RRID:Addgene_139322 Copy
Species: Homo sapiens
Genetic Insert: sgRNA to insert BRCA2 E2772K using base editing
Vector Backbone Description: Backbone Size:2633; Vector Backbone:B52 (Addgene #100708); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32160537
Proper citation: RRID:Addgene_139323 Copy
Species: Homo sapiens
Genetic Insert: sgRNA to insert BRCA1 T922I using base editing
Vector Backbone Description: Backbone Size:2633; Vector Backbone:B52 (Addgene #100708); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32160537
Proper citation: RRID:Addgene_139328 Copy
Species: Homo sapiens
Genetic Insert: sgRNA to insert BRCA1 E638K using base editing
Vector Backbone Description: Backbone Size:2633; Vector Backbone:B52 (Addgene #100708); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32160537
Proper citation: RRID:Addgene_139327 Copy
Species: Homo sapiens
Genetic Insert: FLAG-tag NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform)
Vector Backbone Description: Backbone Marker:PMID: 24048477; Vector Backbone:RRL.sin.cPPT.CMV/IRES-puro.WPRE; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30478388
Proper citation: RRID:Addgene_139447 Copy
Species: Homo sapiens
Genetic Insert: sgRNA to insert BRCA1 Q1779Stop using base editing
Vector Backbone Description: Backbone Size:2633; Vector Backbone:B52 (Addgene #100708); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32160537
Proper citation: RRID:Addgene_139331 Copy
Species: Homo sapiens
Genetic Insert: sgRNA to insert FANCD2 Q223Stop using base editing
Vector Backbone Description: Backbone Size:2633; Vector Backbone:B52 (Addgene #100708); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32160537
Proper citation: RRID:Addgene_139332 Copy
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