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Species: Mus musculus
Genetic Insert: Unc5c.y
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Unc5c.y has a 57 nt insertion between exons 7 and 8.
Proper citation: RRID:Addgene_72181 Copy
Species: Mus musculus
Genetic Insert: Unc5d.a
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: We identified two previously-unreported isoforms and named them "y" and "z". As such, we designated this previously-reported isoform as isoform "a".
Proper citation: RRID:Addgene_72183 Copy
Species: Mus musculus
Genetic Insert: Ten-Eleven Translocation 3
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Modified pcDNA3 vector with a beta globulin intron inserted. This insertion can help stabilize the transcripts and is particularly useful for the expression of large genes, such as p300 and Tet proteins
Note that an additional mutation, D1507E, is present in the Tet3 insert in this plasmid. This additional mutation does not affect the property of the mutant protein (as it is catalytically dead).
For additional information about how this plasmid was created and used, please see Ito et al., Nature 2010 Pubmed ID: 20639862
Proper citation: RRID:Addgene_72222 Copy
Species: Mus musculus
Genetic Insert: MEK1/2
Vector Backbone Description: Backbone Size:7032; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_72570 Copy
Species: Mus musculus
Genetic Insert: MEK1/2
Vector Backbone Description: Backbone Size:7032; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_72568 Copy
Species: Mus musculus
Genetic Insert: mouse TNF-alpha shRNA
Vector Backbone Description: Backbone Size:7650; Vector Backbone:pLL3.7; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_72598 Copy
Species: Mus musculus
Genetic Insert: Gata 6
Vector Backbone Description: Backbone Size:11391; Vector Backbone:pSAM2_GW_mCherry; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_72694 Copy
Species: Mus musculus
Genetic Insert: Mesp 2
Vector Backbone Description: Backbone Size:11391; Vector Backbone:pSAM2_GW_mCherry; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_72693 Copy
Species: Mus musculus
Genetic Insert: PACSIN1
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pRK5; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_72577 Copy
Species: Mus musculus
Genetic Insert: PACSIN1
Vector Backbone Description: Backbone Marker:Oligoengine; Backbone Size:3176; Vector Backbone:pSuper; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_72576 Copy
Species: Mus musculus
Genetic Insert: Dub3 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4242; Vector Backbone:pGL4.10[luc]; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: Addgene NGS found a small number of single nucleotide discrepancies relative to Sanger sequencing results provided by the depositing laboratory. These do not affect plasmid function
Proper citation: RRID:Addgene_72684 Copy
Species: Mus musculus
Genetic Insert: Dub3
Vector Backbone Description: Vector Backbone:pcDNA3-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Addgene Sanger sequencing detected an S4A mutation within the Dub3 translation that should not affect protein function
Proper citation: RRID:Addgene_72682 Copy
Species: Mus musculus
Genetic Insert: Tbx 5
Vector Backbone Description: Backbone Size:11391; Vector Backbone:pSAM2_GW_mCherry; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_72688 Copy
Species: Mus musculus
Genetic Insert: Nkx 2.5
Vector Backbone Description: Backbone Size:11391; Vector Backbone:pSAM2_GW_mCherry; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_72689 Copy
Species: Mus musculus
Genetic Insert: FAM20C
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:5500; Vector Backbone:pcDNA3.1/V5-HisB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Full length mouse FAM20C extending 98 bp upstream of the ATG and excluding the stop codon, was amplified from FAM20C mouse BC025826 cDNA by PCR using primers FAM20C-HindIII-F and FAM20C-NotI-R and the resulting fragment was cloned into HindIII and NotI cut pcDNA3.1/V5-His-B to create above plasmid.
Proper citation: RRID:Addgene_63584 Copy
Species: Mus musculus
Genetic Insert: NFAT1
Vector Backbone Description: Backbone Marker:Anjana Rao lab; Backbone Size:6000; Vector Backbone:pRV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_63669 Copy
Species: Mus musculus
Genetic Insert: Gcn5
Vector Backbone Description: Backbone Size:6300; Vector Backbone:pMSCVpuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_63706 Copy
Species: Mus musculus
Genetic Insert: Nanog homologous arm, p2A, NLS, sfGFP, Nanog homologous arm
Vector Backbone Description: Backbone Size:2241; Vector Backbone:pUC19; Vector Types:Just a donor DNA with Nanog homologous arm for knock-in sfGFP; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_63709 Copy
Species: Mus musculus
Genetic Insert: sgNanog, CMV promoter, puro, mCherry
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pHR; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_63710 Copy
Species: Mus musculus
Genetic Insert: Tet2 Promoter fragment F
Vector Backbone Description: Backbone Size:4793; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_63879 Copy
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