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Species: Homo sapiens
Genetic Insert: UbcH5B
Vector Backbone Description: Backbone Marker:Novagen, modified; Backbone Size:6000; Vector Backbone:PETSUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_60443 Copy
Species: Homo sapiens
Genetic Insert: human MSI2 (8-193)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pET-22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_60356 Copy
Species: Homo sapiens
Genetic Insert: Pak5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_60533 Copy
Species: Homo sapiens
Genetic Insert: Pak5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
References:
Comments: This plasmid is derived from Addgene plasmid #23850. We changed a1208c, g79c to align with pubmed sequence and added a stop codon.
Proper citation: RRID:Addgene_60532 Copy
Species: Homo sapiens
Genetic Insert: FGFR4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2546; Vector Backbone:pENTR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_60531 Copy
Species: Homo sapiens
Genetic Insert: Pak5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_60535 Copy
Species: Homo sapiens
Genetic Insert: Pak5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_60539 Copy
Species: Homo sapiens
Genetic Insert: Pak5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_60538 Copy
Species: Homo sapiens
Genetic Insert: ProtoSpacer AAVS1-T2
Vector Backbone Description: Backbone Marker:ClonTech; Backbone Size:4685; Vector Backbone:pEGFP C1; Vector Types:Mammalian Expression, CRISPR, Kan; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_60543 Copy
Species: Homo sapiens
Genetic Insert: ZKSCAN1
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:3900; Vector Backbone:pCRII-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_60643 Copy
Species: Homo sapiens
Genetic Insert: ZKSCAN1
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: A 51-nt artificial exon composed of restriction enzyme sites was inserted between the minimal introns that support ZKSCAN1 circular RNA production (as described in Figure 7A of Liang and Wilusz (2014) Genes Dev).
Proper citation: RRID:Addgene_60648 Copy
Species: Homo sapiens
Genetic Insert: Argonaute 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7481; Vector Backbone:pDEST 26; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_60651 Copy
Species: Homo sapiens
Genetic Insert: Argonaute 2
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4969; Vector Backbone:pGEX 4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_60656 Copy
Species: Homo sapiens
Genetic Insert: Argonaute 2
Vector Backbone Description: Backbone Marker:Richard Mulligan; Backbone Size:7000; Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_60655 Copy
Species: Homo sapiens
Genetic Insert: DJ-1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5708; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Internal NdeI site of human DJ-1 eliminated by silent mutation (198A->T)
Proper citation: RRID:Addgene_60678 Copy
Species: Homo sapiens
Genetic Insert: M26I DJ-1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: This construct contains a Tobacco etch virus (TEV) protease recognition sequence (ENLYFQS) after the Thrombin recognition site, and just before DJ-1. (Ex. LVPRGSHMENLYFQSASKRAL......) with the TEV site italicized. Internal NdeI site of human DJ-1 eliminated by silent mutation (198A->T).
Proper citation: RRID:Addgene_60685 Copy
Species: Homo sapiens
Genetic Insert: Beta-actin
Vector Backbone Description: Backbone Marker:Agilent; Vector Backbone:pSG5; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_60618 Copy
Species: Homo sapiens
Genetic Insert: VP64 human MyoD
Vector Backbone Description: Backbone Size:10000; Vector Backbone:pRRL; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_60629 Copy
Species: Homo sapiens
Genetic Insert: ZKSCAN1
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_60631 Copy
Species: Homo sapiens
Genetic Insert: Homeodomain-interacting protein kinase 3
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid is the same as "pcDNA3.1(+) HIPK3 Sense" except that the 2803-nt insert is in the antisense orientation (and thus no circular RNA is produced).
Proper citation: RRID:Addgene_60635 Copy
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