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Species: Homo sapiens
Genetic Insert: beta2-adrenergic receptor
Vector Backbone Description: Backbone Size:5070; Vector Backbone:pCDNA5_FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Notes:
(1) The pCDNA/FRT vector contains an extra XbaI site.
(2) The XbaI restriction enzyme is sensitive to dam methylation.
Proper citation: RRID:Addgene_47438 Copy
Species: Homo sapiens
Genetic Insert: beta2-adrenergic receptor
Vector Backbone Description: Backbone Size:5070; Vector Backbone:pCDNA5_FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Notes:
(1) The pCDNA/FRT vector contains an extra XbaI site.
(2) The XbaI restriction enzyme is sensitive to dam methylation.
(3) No-pep sensor contains a short repeating GSG sequence at the C-terminus of mCerulean.
Proper citation: RRID:Addgene_47439 Copy
Species: Homo sapiens
Genetic Insert: beta2-adrenergic receptor
Vector Backbone Description: Backbone Size:5070; Vector Backbone:pCDNA5_FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Notes:
(1) The pCDNA/FRT vector contains an extra XbaI site.
(2) The XbaI restriction enzyme is sensitive to dam methylation.
Proper citation: RRID:Addgene_47437 Copy
Species: Homo sapiens
Genetic Insert: Intersectin short
Vector Backbone Description: Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_47392 Copy
Species: Homo sapiens
Genetic Insert: Clavesin1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4324; Vector Backbone:pCMV-Tag2B; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_47430 Copy
Species: Homo sapiens
Genetic Insert: intersectin long
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_47395 Copy
Species: Homo sapiens
Genetic Insert: Olfactomedin-like domain with V426F mutation
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:6645; Vector Backbone:pmalC4X; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: The Lieberman Lab recommends E.coli Rosetta Gami 2 competent cells for protein expression.
Proper citation: RRID:Addgene_47702 Copy
Species: Homo sapiens
Genetic Insert: Olfactomedin-like domain with I499F mutation
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:6645; Vector Backbone:pmalC4X; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: The Lieberman Lab recommends E.coli Rosetta Gami 2 competent cells for protein expression.
Proper citation: RRID:Addgene_47703 Copy
Species: Homo sapiens
Genetic Insert: CALML3
Vector Backbone Description: Backbone Marker:Pharmacia; Backbone Size:4580; Vector Backbone:pKK233-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_47599 Copy
Species: Homo sapiens
Genetic Insert: PMCA4b-D672E
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_47596 Copy
Species: Homo sapiens
Genetic Insert: PMCA4bct120
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: x/b splice variant of human PMCA4
Proper citation: RRID:Addgene_47593 Copy
Species: Homo sapiens
Genetic Insert: PMCA4bct125
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: x/b splice variant of human PMCA4
Proper citation: RRID:Addgene_47594 Copy
Species: Homo sapiens
Genetic Insert: PMCA2w/b delta6
Vector Backbone Description: Backbone Marker:Clontech; modified as described in Rogers et al JBC 2005; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_47588 Copy
Species: Homo sapiens
Genetic Insert: PMCA4x/b
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: HPMCA4x/b coding sequence starts with amino acid 2 (Thr) at pos 1354.
This plasmid carries two missense mutations that cause K442R and P663L in the amino acid sequence of hPMCA4b. The depositing lab did not notice any functional consequences in the assays they performed. However, these were mostly concerned with membrane localization and did not include any enzymatic functional studies. Thus, it is not know whether (and how) these two mutations affect the calcium-pumping enzymatic function of the encoded GFP-hPMCA4b protein.
Proper citation: RRID:Addgene_47589 Copy
Species: Homo sapiens
Genetic Insert: PMCA2w/b
Vector Backbone Description: Backbone Marker:Clontech; modified as described in Rogers et al JBC 2003; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: w/b splice variant of human PMCA2. PMCA2w/b inserted as SacII-XbaI fragment into multiple cloning site but is also flanked by NotI sites. PMCA2 start: 1396; stop: 5125, w-splice: 2303
Proper citation: RRID:Addgene_47586 Copy
Species: Homo sapiens
Genetic Insert: PMCA2z/b
Vector Backbone Description: Backbone Marker:Penniston Lab; Backbone Size:5145; Vector Backbone:pMM2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: z/b splice variant of human PMCA2. PMCA2 start: 1104; stop: 4700, z-splice: 2010/11; b-splice: 4389-4700
Proper citation: RRID:Addgene_47581 Copy
Species: Homo sapiens
Genetic Insert: pMCA2z/b
Vector Backbone Description: Backbone Marker:Clontech; modified as described in Rogers et al JBC 2001; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: z/b splice variant of human PMCA2
Proper citation: RRID:Addgene_47584 Copy
Species: Homo sapiens
Genetic Insert: PMCA2x/b
Vector Backbone Description: Backbone Marker:Penniston Lab; Backbone Size:5145; Vector Backbone:pMM2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: x/b splice variant of human PMCA2. PMCA2 start: 1090; stop: 4728, x-splice: 1997-2038; b-splice: starts at 4417.
Proper citation: RRID:Addgene_47582 Copy
Species: Homo sapiens
Genetic Insert: PMCA2w/b
Vector Backbone Description: Backbone Marker:Penniston Lab; Backbone Size:5145; Vector Backbone:pMM2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: w/b splice variant of human PMCA2. PMCA2 start: 1090; stop: 4821, w-splice: 1997-2131; b-splice: 4510-4821
Proper citation: RRID:Addgene_47583 Copy
Species: Homo sapiens
Genetic Insert: MKK4-EE
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5062; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_47577 Copy
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