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Species: Synthetic
Genetic Insert: SaSp Cas9
Vector Backbone Description: Backbone Marker:Genscript; Vector Backbone:pUC57 ; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/2023.10.24.563270 for bioRxiv preprint.
Proper citation: RRID:Addgene_210742 Copy
Species: Synthetic
Genetic Insert: L3S1P13 strong terminator
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_204119 Copy
Species: Synthetic
Genetic Insert: Toxic levansucrase sacB
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_204113 Copy
Species: Synthetic
Genetic Insert: PlmJ ribozyme and MCD2 strong RBS
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_204084 Copy
Species: Synthetic
Genetic Insert: RiboJ ribozyme and MCD2 strong RBS
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_204082 Copy
Species: Synthetic
Genetic Insert: AraJ ribozyme and MCD2 strong RBS
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_204088 Copy
Species: Synthetic
Genetic Insert: L3S1P47 strong terminator
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_204121 Copy
Species: Synthetic
Genetic Insert: CchJ ribozyme MCD2 strong RBS
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_204087 Copy
Species: Synthetic
Genetic Insert: ECK120033736 strong terminator
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_204120 Copy
Species: Synthetic
Genetic Insert: MCD2 strong RBS
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_204090 Copy
Species: Synthetic
Genetic Insert: MCD15 medium RBS
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_204095 Copy
Species: Synthetic
Genetic Insert: MCD8 weak RBS
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_204097 Copy
Species: Synthetic
Genetic Insert: E2-Crimson cassette flanked with homology arms for recombination into the S. alvi wkB2 genome to replace clpA (SALWKB2_RS04465)
Vector Backbone Description: Backbone Size:1887; Vector Backbone:pYTK095; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:37786689
Comments: Please visit https://doi.org/10.1101/2023.09.19.558440 for bioRxiv preprint.
Proper citation: RRID:Addgene_209550 Copy
Species: Synthetic
Genetic Insert: Defective hygromycin resistant gene
Vector Backbone Description: Backbone Marker:Dirk Schnappinger; Backbone Size:3592; Vector Backbone:pDE43-MCZ; Vector Types:Bacterial Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:34235660
Comments: Defective hygromycin-resistant gene was generated by overlap PCR and cloned into the PvuI-HindIII sites of pDE43-MCZ, a mycobacterial phage L5 integrating vector.
Proper citation: RRID:Addgene_210788 Copy
Species: Synthetic
Genetic Insert: Pathways
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35366417
Proper citation: RRID:Addgene_200006 Copy
Species: Synthetic
Genetic Insert: deLACCO1
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37891202
Comments: Please visit https://doi.org/10.1101/2022.12.27.522013 for bioRxiv preprint.
Proper citation: RRID:Addgene_207990 Copy
Species: Synthetic
Genetic Insert: sgCASP8
Vector Backbone Description: Vector Backbone:pLentiCRISPR-v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39592843
Proper citation: RRID:Addgene_211519 Copy
Species: Synthetic
Genetic Insert: sgUCKL1
Vector Backbone Description: Vector Backbone:pLentiCRISPR-v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39592843
Proper citation: RRID:Addgene_211523 Copy
Species: Synthetic
Genetic Insert: sgUCK2
Vector Backbone Description: Vector Backbone:pLentiCRISPR-v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39592843
Proper citation: RRID:Addgene_211522 Copy
Species: Synthetic
Genetic Insert: sgUGP2
Vector Backbone Description: Vector Backbone:pLentiCRISPR-v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39592843
Proper citation: RRID:Addgene_211521 Copy
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