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Species: Other
Genetic Insert: ecTadA(WT)-ecTadA(8e)-nSaCas9
Vector Backbone Description: Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32433547
Proper citation: RRID:Addgene_138501 Copy
Species: Other
Genetic Insert: ecTadA(8e)-dAsCas12a (En)
Vector Backbone Description: Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32433547
Proper citation: RRID:Addgene_138506 Copy
Species: Other
Genetic Insert: T7-RNAP
Vector Backbone Description: Vector Backbone:pCMV with a BR322 origin; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32433547
Proper citation: RRID:Addgene_138514 Copy
Species: Other
Genetic Insert: T7-RNAP
Vector Backbone Description: Vector Backbone:pCMV with a BR322 origin; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32433547
Proper citation: RRID:Addgene_138517 Copy
Species: Other
Genetic Insert: T7-RNAP
Vector Backbone Description: Vector Backbone:pCMV with a BR322 origin; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32433547
Proper citation: RRID:Addgene_138519 Copy
Species: Other
Genetic Insert: T7-RNAP
Vector Backbone Description: Vector Backbone:pCMV with a BR322 origin; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32433547
Proper citation: RRID:Addgene_138518 Copy
Species: Other
Genetic Insert: T7-RNAP
Vector Backbone Description: Vector Backbone:pCMV with a BR322 origin; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32433547
Proper citation: RRID:Addgene_138520 Copy
Species: Other
Genetic Insert: phage genes
Vector Backbone Description: Vector Backbone:pCMV with a BR322 origin; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32433547
Proper citation: RRID:Addgene_138523 Copy
Species: Other
Genetic Insert: ecTadA(8e)-nSpCas9 (CP1028)
Vector Backbone Description: Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32433547
Proper citation: RRID:Addgene_138492 Copy
Species: Other
Genetic Insert: ecTadA(8e V82G)-nSpCas9
Vector Backbone Description: Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32433547
Proper citation: RRID:Addgene_138494 Copy
Species: Other
Genetic Insert: ecTadA(8e K20A R21A)-nSpCas9
Vector Backbone Description: Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32433547
Proper citation: RRID:Addgene_138496 Copy
Species: Other
Genetic Insert: ecTadA(8e V106W)-nSpCas9
Vector Backbone Description: Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32433547
Proper citation: RRID:Addgene_138495 Copy
Species: Other
Genetic Insert: ecTadA(WT)-ecTadA(8e)-nSpCas9 (CP1028)
Vector Backbone Description: Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32433547
Proper citation: RRID:Addgene_138498 Copy
Species: Other
Genetic Insert: ecTadA(WT)-ecTadA(8e)-nSpCas9 (CP1041)
Vector Backbone Description: Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32433547
Proper citation: RRID:Addgene_138499 Copy
Species: Other
Genetic Insert: Mus musculus Akap1(1-31) M16L: PTRSANS[GA]4ILSRM:TagBFP2: FKBP1A(3-108): [GGSA]4GG:L. monocytogenes PI-PLC(1-187)
Vector Backbone Description: Vector Backbone:pTagBFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32211893
Proper citation: RRID:Addgene_139309 Copy
Species: Other
Genetic Insert: SNIPR_GFP
Vector Backbone Description: Backbone Size:3224; Vector Backbone:pCOLAduet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32109416
Comments: SNIPR regulating GFPmut3b with an ASV degradation tag
Proper citation: RRID:Addgene_139463 Copy
Species: Other
Genetic Insert: SNIPR_lacZ
Vector Backbone Description: Backbone Size:3324; Vector Backbone:pCOLAduet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32109416
Comments: SNIPR regulating a lacZ reporter protein
Proper citation: RRID:Addgene_139464 Copy
Species: Other
Genetic Insert: Target_mutGU33
Vector Backbone Description: Backbone Size:5527; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32109416
Comments: Non-cognate target for the SNIPR with a mismatch at base 33 leading to a G-U base pair with SNIPR
Proper citation: RRID:Addgene_139468 Copy
Species: Other
Genetic Insert: loxp-DsRed-loxp-eGFP
Vector Backbone Description: Backbone Marker:Eric Campeau & Paul Kaufman (Addgene plasmid # 17294); Vector Backbone:pLenti CMV/TO Zeo DEST (644-1) ; Vector Types:Lentiviral; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:31649238
Comments: loxp-DsRed-loxp-eGFP was PCR amplified from pMSCV-loxp-DsRed-loxp-eGFP-Puro-WPRE (Addgene plasmid #32702) using primers containing Sal1 and Not1 restriction enzyme sites. The amplified PCR fragment was digested with Sal1 and Not1 and ligated into the pENTRA1 vector cut with Sal1 and Not1. The Gateway SystemTM (Invitrogen) was used to recombine the pENTR1A shuttle vector with pLenti CMV/TO Zeo DEST (644-1) (#17294, Addgene) generating lentiviral vector 1.
Proper citation: RRID:Addgene_141148 Copy
Species: Other
Genetic Insert: 4xHRE-MinTK-CRE
Vector Backbone Description: Backbone Marker:Didier Trono (Addgene plasmid # 12252); Vector Backbone:pRRLSIN.cPPT.PGK-GFP.WPRE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31649238
Proper citation: RRID:Addgene_141144 Copy
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