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Species: Escherichia coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
References:
Comments: This plasmid carries a UAG nonsense mutation near the 5' end of lacZ.
Proper citation: RRID:Addgene_63870 Copy
Species: Escherichia coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
References:
Comments: This plasmid carries a UAG nonsense mutation near the 5' end of lacZ.
Proper citation: RRID:Addgene_63871 Copy
Species: Escherichia coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
References:
Comments: This plasmid carries a UAA nonsense mutation near the 5' end of lacZ.
Proper citation: RRID:Addgene_63872 Copy
Species: Escherichia coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
References:
Comments: This plasmid carries a UAA nonsense mutation near the 5' end of lacZ.
Proper citation: RRID:Addgene_63873 Copy
Species: Escherichia coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
References:
Comments: AUG --> GUG start codon in lacZ
Proper citation: RRID:Addgene_63866 Copy
Species: Escherichia coli
Genetic Insert: phosphoribosylglycinamide synthetase
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Constructed by Danny Crawford and T. J. Kappock in the laboratory of JoAnne Stubbe (MIT).
In JM109 strain.
Proper citation: RRID:Addgene_75077 Copy
Species: Escherichia coli
Genetic Insert: NptII
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Backbone Size:6698; Vector Backbone:pDGB3omega1R; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_75471 Copy
Species: Escherichia coli
Genetic Insert: HpaB
Vector Backbone Description: Backbone Marker:Addgene Plasmid # 49795 ; Backbone Size:5155; Vector Backbone:pETM6; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_71260 Copy
Species: Escherichia coli
Genetic Insert: mutL E32K
Vector Backbone Description: Backbone Marker:Datta S. et al. (Gene 379 (2006) 109-115); Backbone Size:6248; Vector Backbone:pSIM8; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit the depositor's website: http://group.szbk.u-szeged.hu/sysbiol/pal-csaba-lab-resources.html for additional resource information.
Proper citation: RRID:Addgene_72680 Copy
Species: Escherichia coli
Genetic Insert: ORF17_DHNTPase_NUD
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET11b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_73154 Copy
Species: Escherichia coli
Genetic Insert: guanylate kinase
Vector Backbone Description: Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Source was E. coli K-12 MRE6000
Proper citation: RRID:Addgene_73631 Copy
Species: Escherichia coli
Genetic Insert: formylglycinamidine ribonucleotide synthetase
Vector Backbone Description: Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Repaired form of a plasmid described originally in Schultheisz HL, Szymczyna BR, Scott LG, Williamson JR (2008) Pathway engineered enzymatic de novo purine nucleotide synthesis. ACS Chem Biol. 3, 499-511. doi: 10.1021/cb800066p
Proper citation: RRID:Addgene_74277 Copy
Species: Escherichia coli
Genetic Insert: clpX
Vector Backbone Description: Vector Backbone:pJKR GFP; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_89657 Copy
Species: Escherichia coli
Genetic Insert: GTP-binding tubulin-like cell division protein
Vector Backbone Description: Vector Backbone:pJKR GFP; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_89656 Copy
Species: Escherichia coli
Genetic Insert: rpsA
Vector Backbone Description: Backbone Size:4520; Vector Backbone:pCA24N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_83585 Copy
Species: Escherichia coli
Genetic Insert: Tus
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5240; Vector Backbone:pET24b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: The plasmid backbone is originally pET24b-APEX2 (Plasmid #111702) acquired from Addgene. In this submission, the Apex2 insert has been replaced with NusA-Tus.
Proper citation: RRID:Addgene_165959 Copy
Species: Escherichia coli
Genetic Insert: tonB C18G, R204C
Vector Backbone Description: Vector Backbone:pPro33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Parent plasmid is pKP1362 in this collection. Please note that some Genbank listings and Snapgene's ORF recognition software may add additional amino acids to the TonB ORF. Postle and Skare, 1988, PMID: 2839513 demonstrated that the first two Met residues of the open reading frame are not translated and that TonB starts with the third ATG codon. The mutations listed above are numbered based on the functional starting codon.
Proper citation: RRID:Addgene_167620 Copy
Species: Escherichia coli
Genetic Insert: tonB C18G, H20A, N208C
Vector Backbone Description: Vector Backbone:pPro33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Parent plasmid is pKP1362 in this collection. Please note that some Genbank listings and Snapgene's ORF recognition software may add additional amino acids to the TonB ORF. Postle and Skare, 1988, PMID: 2839513 demonstrated that the first two Met residues of the open reading frame are not translated and that TonB starts with the third ATG codon. The mutations listed above are numbered based on the functional starting codon.
Proper citation: RRID:Addgene_167625 Copy
Species: Escherichia coli
Genetic Insert: tonB C18G, N208C
Vector Backbone Description: Vector Backbone:pPro33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Parent plasmid is pKP1362 in this collection. Please note that some Genbank listings and Snapgene's ORF recognition software may add additional amino acids to the TonB ORF. Postle and Skare, 1988, PMID: 2839513 demonstrated that the first two Met residues of the open reading frame are not translated and that TonB starts with the third ATG codon. The mutations listed above are numbered based on the functional starting codon.
Proper citation: RRID:Addgene_167624 Copy
Species: Escherichia coli
Genetic Insert: tonB C18G, H20A, R212C
Vector Backbone Description: Vector Backbone:pPro33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Parent plasmid is pKP1362 in this collection. Please note that some Genbank listings and Snapgene's ORF recognition software may add additional amino acids to the TonB ORF. Postle and Skare, 1988, PMID: 2839513 demonstrated that the first two Met residues of the open reading frame are not translated and that TonB starts with the third ATG codon. The mutations listed above are numbered based on the functional starting codon.
Proper citation: RRID:Addgene_167629 Copy
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