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Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_190156 Copy
Species: Pseudomonas aeruginosa UCBPP-PA14
Genetic Insert: PaCas1, PaCas2-3
Vector Backbone Description: Backbone Size:2819; Vector Backbone:2S; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_200213 Copy
Species: Pseudomonas aeruginosa UCBPP-PA14
Genetic Insert: PaCas1, PaCas2-3
Vector Backbone Description: Backbone Size:2819; Vector Backbone:2S; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_200214 Copy
Species: Pseudomonas aeruginosa UCBPP-PA14
Genetic Insert: PaCas1, PaCas2-3
Vector Backbone Description: Backbone Size:2819; Vector Backbone:2S; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_200215 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pK7WG2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: Multisite Gateway destination vector to express ORFs from entry clones. RPS5A promoter and SV40 nuclear localization signal in front of attR1. HSP terminator at the downstream of attR5. Kanamycin-resistant gene and Ole1-GFP for screening of transformants. Please visit https://doi.org/10.1101/2023.02.27.530312 for bioRxiv preprint.
Proper citation: RRID:Addgene_193370 Copy
Species: Mus musculus
Genetic Insert: Dishevelled-2
Vector Backbone Description: Backbone Marker:Novagen/EMD Biosciences; Vector Backbone:pCDFduet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_216387 Copy
Species: Synthetic
Genetic Insert: RedNTF
Vector Backbone Description: Vector Backbone:pGreen; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_198421 Copy
Species: Xenorhabdus nematophila ATCC 19061
Genetic Insert: NrdJ-1C - XtpS
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: This plasmid encodes the termination unit of the non-ribosomal peptide synthetase (NRPS) called xenotetrapeptide synthetase (XtpS), split at an XUTII site and fused to the C-terminal part of the split intein NrdJ-1.When co-expressed with pIN092 and pIN201, the three fragments splice into a functional NRPS that produces the non-ribosomal peptide (NRP) called xenotetrapeptide (XTP; cyclo[Leu-D-Val-Val-D-Val]; CC(C)C[C@H]1C(=O)N[C@@H](C(=O)N[C@H](C(=O)N[C@@H](C(=O)N1)C(C)C)C(C)C)C(C)C; 410.6 g/mol).
For successful NRP production, the three plasmids must be co-transformed into an E. coli strain that expresses a broad-spectrum phosphopantetheinyl transferase, such as MtaA (DH10B::mtaA) or Sfp (BAP1), which is required for the post-translational activation of the T domains.
Expression is controlled by an araBAD promoter, inducible with 0.2% arabinose.
Proper citation: RRID:Addgene_255796 Copy
Species: Xenorhabdus nematophila ATCC 19061
Genetic Insert: SspGyrBC - XtpS
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: This plasmid encodes the termination unit of the non-ribosomal peptide synthetase (NRPS) called xenotetrapeptide synthetase (XtpS), split at an XU site and fused to the C-terminal part of the split intein SspGyrB.When co-expressed with pIN103 and pIN203, the three fragments splice into a functional NRPS that produces the non-ribosomal peptide (NRP) called xenotetrapeptide (XTP; cyclo[Leu-D-Val-Val-D-Val]; CC(C)C[C@H]1C(=O)N[C@@H](C(=O)N[C@H](C(=O)N[C@@H](C(=O)N1)C(C)C)C(C)C)C(C)C; 410.6 g/mol).
For successful NRP production, the three plasmids must be co-transformed into an E. coli strain that expresses a broad-spectrum phosphopantetheinyl transferase, such as MtaA (DH10B::mtaA) or Sfp (BAP1), which is required for the post-translational activation of the T domains.
Expression is controlled by an araBAD promoter, inducible with 0.2% arabinose.
Proper citation: RRID:Addgene_255798 Copy
Species:
Genetic Insert: Synpcc7942_1849
Vector Backbone Description: Vector Backbone:Vector for targeting integration at neutral site 1 in ATCC PCC7942 genome; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_119795 Copy
Species:
Genetic Insert: Synpcc7942_1557
Vector Backbone Description: Vector Backbone:Vector for targeting integration at neutral site 1 in ATCC PCC7942 genome; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_119796 Copy
Species: Broad range of genetically tractable cyanobacteria
Genetic Insert: RSF1010 Y25F aadA PconII theophylline riboswitch F with myc-yfp reporter
Vector Backbone Description: Vector Backbone:RSF1010Y25F-AADA; Vector Types:Other; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_120085 Copy
Species: Synechococcus elongatus PCC 7942
Genetic Insert: pAM4788 where the promoter/reporter device (PconII-LTRBS-yemGFP) was replaced with a MCS from the pBluescript vector (pBS MCS).
Vector Backbone Description: Backbone Size:6936; Vector Backbone:pAM4788; Vector Types:other; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_120087 Copy
Species: Synechococcus elongatus PCC 7942
Genetic Insert: One Step Cloneing Vector for Overexpression
Vector Backbone Description: Vector Backbone:pAM2990; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: pAM2990 was partially digested with SacI and re-ligatedto remove the antihel fragment. The net result is the insertion of the BglII fragment from pAM2255 into BamHI digested pAM1303 in the orientation that Ptrc goes away from the SacI site in pAM1303 (SacI digestion gives a 1.7 KB band). This plasmid can be used as a one-step cloning vector for overexpression.
Proper citation: RRID:Addgene_40248 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: Addgene NGS was unable to completely resolve all bases in the CloDF13 Origin. The plasmid has been successfully propagated at Addgene and these ambiguous bases do not appear to have a functional impact on the plasmid
Proper citation: RRID:Addgene_160171 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: Addgene NGS was unable to completely resolve all bases in the CloDF13 Origin. The plasmid has been successfully propagated at Addgene and these ambiguous bases do not appear to have a functional impact on the plasmid
Proper citation: RRID:Addgene_160169 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: Addgene NGS was unable to completely resolve all bases in the CloDF13 Origin. The plasmid has been successfully propagated at Addgene and these ambiguous bases do not appear to have a functional impact on the plasmid
Proper citation: RRID:Addgene_160261 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: Addgene NGS was unable to completely resolve all bases in the CloDF13 Origin. The plasmid has been successfully propagated at Addgene and these ambiguous bases do not appear to have a functional impact on the plasmid
Proper citation: RRID:Addgene_160262 Copy
Species: Other
Genetic Insert: aadA
Vector Backbone Description: Backbone Marker:-; Backbone Size:7000; Vector Backbone:pAM5406; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_132663 Copy
Species: Synthetic
Genetic Insert: sgRNA-FRT
Vector Backbone Description: Vector Backbone:pTargetF; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_113637 Copy
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