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http://www.addgene.org/128404

Species: P. aeruginosa
Genetic Insert: Exotoxin A
Vector Backbone Description: Backbone Size:4535; Vector Backbone:pTrc99S; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:

Proper citation: RRID:Addgene_128404 Copy   

•   Source: Addgene

http://www.addgene.org/128399

Species: H. influenzae
Genetic Insert: H. influenzae protein D
Vector Backbone Description: Backbone Size:4535; Vector Backbone:pTrc99S; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:

Proper citation: RRID:Addgene_128399 Copy   

•   Source: Addgene

http://www.addgene.org/119795

Species:
Genetic Insert: Synpcc7942_1849
Vector Backbone Description: Vector Backbone:Vector for targeting integration at neutral site 1 in ATCC PCC7942 genome; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:

Proper citation: RRID:Addgene_119795 Copy   

•   Source: Addgene

http://www.addgene.org/119796

Species:
Genetic Insert: Synpcc7942_1557
Vector Backbone Description: Vector Backbone:Vector for targeting integration at neutral site 1 in ATCC PCC7942 genome; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:

Proper citation: RRID:Addgene_119796 Copy   

•   Source: Addgene

http://www.addgene.org/120085

Species: Broad range of genetically tractable cyanobacteria
Genetic Insert: RSF1010 Y25F aadA PconII theophylline riboswitch F with myc-yfp reporter
Vector Backbone Description: Vector Backbone:RSF1010Y25F-AADA; Vector Types:Other; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:

Proper citation: RRID:Addgene_120085 Copy   

•   Source: Addgene

http://www.addgene.org/120087

Species: Synechococcus elongatus PCC 7942
Genetic Insert: pAM4788 where the promoter/reporter device (PconII-LTRBS-yemGFP) was replaced with a MCS from the pBluescript vector (pBS MCS).
Vector Backbone Description: Backbone Size:6936; Vector Backbone:pAM4788; Vector Types:other; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:

Proper citation: RRID:Addgene_120087 Copy   

•   Source: Addgene

http://www.addgene.org/123186

Species: Catharanthus roseus
Genetic Insert: Catharanthus roseus STR1 promoter and 5'UTR
Vector Backbone Description: Backbone Marker:Weber et al., 2011 (DOI:10.1371/journal.pone.0016765); Backbone Size:2247; Vector Backbone:pICH41295; Vector Types:Plant Expression, part for plant expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: Golden Gate (MoClo; PRO + 5U) compatible STR1 promoter from Catharanthus roseus. Can be used as a control for transactivation experiments. Confirmed activation through ORCA3 and repression through ZCT1

Proper citation: RRID:Addgene_123186 Copy   

•   Source: Addgene

http://www.addgene.org/123185

Species: Catharanthus roseus
Genetic Insert: ORCA3 from Catharanthus roseus
Vector Backbone Description: Backbone Marker:Weber et al., 2011 (DOI:10.1371/journal.pone.0016765); Backbone Size:2247; Vector Backbone:pICH41308; Vector Types:Plant Expression, part for plant expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: Golden Gate (MoClo - Weber et al., 2011), CDS1 position, Octadecanoid-Responsive Catharanthus AP2 (ORCA3, GenBank: EU072424.1); activator of the Catharanthus roseus STR1 promoter

Proper citation: RRID:Addgene_123185 Copy   

•   Source: Addgene

http://www.addgene.org/239459

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pMCY2; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: Two cloning steps are required for the generation of a "promoter:3xHA:VENUS:GENE:2A:mTurqN7" fragment: A) Cloning of promoter: Primer design: Forward Primer: 5’GATCGGGGCGCGCCCTCGAG+PROMOTER SPECIFIC SEQUENCE 3’ Reverse primer: 5’ GATGGGCATTAACATCTTTTAC+PROMOTER SPECIFIC SEQUENCE 3' Digest plasmid with SapI Clean Perform Gibson Assembly Transform E. coli and select positive colonies LB+Sp/Sm (blue/white selection) B) Cloning of gene fragment: Forward Primer: 5’GTTCTGGTGGGGGTGGCTCC+ GENE SPECIFIC SEQUENCE (starting from the ATG) 3’ Reverse primer:  5’ AACAGCTGGCCCGAGCCACC+GENE SPECIFIC SEQUENCE (without STOP Codon) 3' Digest plasmid with Bsu36I and perform gel purification Perform Gibson Assembly Transform E. coli and select positive colonies LB+Sp/Sm The discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences.

Proper citation: RRID:Addgene_239459 Copy   

•   Source: Addgene

http://www.addgene.org/200213

Species: Pseudomonas aeruginosa UCBPP-PA14
Genetic Insert: PaCas1, PaCas2-3
Vector Backbone Description: Backbone Size:2819; Vector Backbone:2S; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:

Proper citation: RRID:Addgene_200213 Copy   

•   Source: Addgene

http://www.addgene.org/200214

Species: Pseudomonas aeruginosa UCBPP-PA14
Genetic Insert: PaCas1, PaCas2-3
Vector Backbone Description: Backbone Size:2819; Vector Backbone:2S; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:

Proper citation: RRID:Addgene_200214 Copy   

•   Source: Addgene

http://www.addgene.org/200215

Species: Pseudomonas aeruginosa UCBPP-PA14
Genetic Insert: PaCas1, PaCas2-3
Vector Backbone Description: Backbone Size:2819; Vector Backbone:2S; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:

Proper citation: RRID:Addgene_200215 Copy   

•   Source: Addgene

http://www.addgene.org/239460

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pMCY2; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: For cloning a promoter + gene fragment (without stop codon) via Gibson Assembly, we recommend the following approach: Primer design: Forward primer: 5’GATCGGGGCGCGCCCTCGAG+PROMOTER SPECIFIC PRIMER 3’ Reverse primer:  5’ CAGAACCACCACCTCCGGATCC+GENE SPECIFIC PRIMER W/O STOP CODON 3' Digest pMDS1 plasmid with SAP I enzyme Clean up Perform Gibson assembly reaction Transform E. coli (DH5alpha) Select on LB + Sp + Sm

Proper citation: RRID:Addgene_239460 Copy   

•   Source: Addgene

http://www.addgene.org/255796

Species: Xenorhabdus nematophila ATCC 19061
Genetic Insert: NrdJ-1C - XtpS
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: This plasmid encodes the termination unit of the non-ribosomal peptide synthetase (NRPS) called xenotetrapeptide synthetase (XtpS), split at an XUTII site and fused to the C-terminal part of the split intein NrdJ-1.When co-expressed with pIN092 and pIN201, the three fragments splice into a functional NRPS that produces the non-ribosomal peptide (NRP) called xenotetrapeptide (XTP; cyclo[Leu-D-Val-Val-D-Val]; CC(C)C[C@H]1C(=O)N[C@@H](C(=O)N[C@H](C(=O)N[C@@H](C(=O)N1)C(C)C)C(C)C)C(C)C; 410.6 g/mol). For successful NRP production, the three plasmids must be co-transformed into an E. coli strain that expresses a broad-spectrum phosphopantetheinyl transferase, such as MtaA (DH10B::mtaA) or Sfp (BAP1), which is required for the post-translational activation of the T domains. Expression is controlled by an araBAD promoter, inducible with 0.2% arabinose.

Proper citation: RRID:Addgene_255796 Copy   

•   Source: Addgene

http://www.addgene.org/255798

Species: Xenorhabdus nematophila ATCC 19061
Genetic Insert: SspGyrBC - XtpS
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: This plasmid encodes the termination unit of the non-ribosomal peptide synthetase (NRPS) called xenotetrapeptide synthetase (XtpS), split at an XU site and fused to the C-terminal part of the split intein SspGyrB.When co-expressed with pIN103 and pIN203, the three fragments splice into a functional NRPS that produces the non-ribosomal peptide (NRP) called xenotetrapeptide (XTP; cyclo[Leu-D-Val-Val-D-Val]; CC(C)C[C@H]1C(=O)N[C@@H](C(=O)N[C@H](C(=O)N[C@@H](C(=O)N1)C(C)C)C(C)C)C(C)C; 410.6 g/mol). For successful NRP production, the three plasmids must be co-transformed into an E. coli strain that expresses a broad-spectrum phosphopantetheinyl transferase, such as MtaA (DH10B::mtaA) or Sfp (BAP1), which is required for the post-translational activation of the T domains. Expression is controlled by an araBAD promoter, inducible with 0.2% arabinose.

Proper citation: RRID:Addgene_255798 Copy   

•   Source: Addgene

http://www.addgene.org/251363

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pDFC; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:

Proper citation: RRID:Addgene_251363 Copy   

•   Source: Addgene

http://www.addgene.org/247988

Species: Synthetic
Genetic Insert: CLOMELEON (CFP-YFP fusion with ENLYFQG-linker)
Vector Backbone Description: Backbone Marker:Andrew Gleave; Backbone Size:11612; Vector Backbone:pART27; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: pART27::CLOMELEON is a binary pART27 vector harbouring a NotI-fragment with a gene cassette subcloned from pART7::CLOMELEON to stably insert the CLOMELEON gene (Kuner, T. & Augustine, G.J.; PubMed: 11055428; doi: 10.1016/S0896-6273(00)00056-8) into the genome of plants by Agrobacterium-mediated gene transfer, e.g. by floral dip (Clough & Bent 1998; PubMed: 10069079; doi: 10.1007/BF00028910). See the Supplemental Document under Resource Information for more details, including annotated maps.

Proper citation: RRID:Addgene_247988 Copy   

•   Source: Addgene

http://www.addgene.org/190156

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:

Proper citation: RRID:Addgene_190156 Copy   

•   Source: Addgene

http://www.addgene.org/198421

Species: Synthetic
Genetic Insert: RedNTF
Vector Backbone Description: Vector Backbone:pGreen; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:

Proper citation: RRID:Addgene_198421 Copy   

•   Source: Addgene

http://www.addgene.org/193370

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pK7WG2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: Multisite Gateway destination vector to express ORFs from entry clones. RPS5A promoter and SV40 nuclear localization signal in front of attR1. HSP terminator at the downstream of attR5. Kanamycin-resistant gene and Ole1-GFP for screening of transformants. Please visit https://doi.org/10.1101/2023.02.27.530312 for bioRxiv preprint.

Proper citation: RRID:Addgene_193370 Copy   

•   Source: Addgene