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Species: Mus musculus
Genetic Insert: Ten-Eleven Translocation 1
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3*; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: *modified pcDNA3 vector with a beta globulin intron inserted. This insertion can help stabilize the transcripts and is particularly useful for the expression of large genes, such as p300 and Tet proteins
Proper citation: RRID:Addgene_60938 Copy
Species: Mus musculus
Genetic Insert: mouse BIN1 with exon 13, excluding exon 7, 11, 14-17
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:8123; Vector Backbone:pDEST27; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_61087 Copy
Species: Mus musculus
Genetic Insert: mouse BIN1 with exon 13 and 17, excluding exon 7, 11, 14-16
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:8123; Vector Backbone:pDEST27; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_61097 Copy
Species: Mus musculus
Genetic Insert: Ngn3
Vector Backbone Description: Backbone Marker:lifetechnologies; Backbone Size:36686; Vector Backbone:pAd CMV/V5-DEST; Vector Types:Adenoviral; Bacterial Resistance:Ampicillin
References:
Comments: For more information, please see the associated article (Li, et al., Nat Biotechnol. 2014 PMID: 25402613.), as well as Li, et al., eLife. 2014, PMID: 24714494.
Note: The plasmid contains an N103Y amino acid residue substitution in the mouse Pdx1 gene. This residue change was present in the depositor's original plasmid stock as described in the associated publication and and should not affect plasmid function.
Proper citation: RRID:Addgene_61041 Copy
Species: Mus musculus
Genetic Insert: TUG
Vector Backbone Description: Backbone Marker:Bogan lab, unpublished; Backbone Size:7233; Vector Backbone:pBICD2; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: The CD2 transmembrane and extracellular sequence are encoded in the vector, after the IRES, and used for selection in FACS (Liu et al Analytical Biochem 2000, PMID 10805516).
Proper citation: RRID:Addgene_52873 Copy
Species: Mus musculus
Genetic Insert: Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3262; Vector Backbone:pSP64; Vector Types:Xenopus Oocyte Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53060 Copy
Species: Mus musculus
Genetic Insert: Lactaherin
Vector Backbone Description: Backbone Size:3648; Vector Backbone:pPD 49.83; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
References:
Comments: Mutations in Lactadherin described in
C. Shao, V.A. Novakovic, J.F. Head, B.A. Seaton, G.E. Gilbert
Crystal structure of lactadherin C2 domain at 1.7A resolution with mutational and computational analyses of its membrane-binding motif
J. Biol. Chem., 283 (2008), pp. 7230–7241
Proper citation: RRID:Addgene_53086 Copy
Species: Mus musculus
Genetic Insert: LOV(wt)-6aa-DAD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5238; Vector Backbone:pTriEx4; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: Expresses photoactivatable (PA) Diaphanous auto-regulatory domain (DAD) consisting of a fusion of mVenus, LOV2 Jα protein domain of Avena sativa Phototropin1, and Diaphanous auto-regulatory domain of mouse Diaphanous-related formin-1 (mDia1)
Proper citation: RRID:Addgene_53095 Copy
Species: Mus musculus
Genetic Insert: LOV(dark)-6aa-DAD
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5238; Vector Backbone:pTriEx4; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: Expresses photoactivatable (PA) Diaphanous auto-regulatory domain (DAD) consisting of a fusion of mVenus, dark LOV2 Jα protein domain of Avena sativa Phototropin1, and Diaphanous auto-regulatory domain of mouse Diaphanous-related formin-1 (mDia1)
Please note that the C450M mutation in LOV2 creates a "dark state" mutant. See Yi et al., ACS Synth Biol. 2014 Nov 21;3(11):788-95 for more information.
Proper citation: RRID:Addgene_53096 Copy
Species: Mus musculus
Genetic Insert: Par1
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescriptSK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53227 Copy
Species: Mus musculus
Genetic Insert: Par4
Vector Backbone Description: Vector Backbone:pBJKS(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53232 Copy
Species: Mus musculus
Genetic Insert: Par3
Vector Backbone Description: Backbone Marker:Mark Davis lab; Vector Backbone:pBJ1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53230 Copy
Species: Mus musculus
Genetic Insert: PRDM16
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4970; Vector Backbone:pGEX4T-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53346 Copy
Species: Mus musculus
Genetic Insert: muscle creatine kinase promoter/enhancer 1.35kb
Vector Backbone Description: Backbone Size:4361; Vector Backbone:pBR322; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53368 Copy
Species: Mus musculus
Genetic Insert: HA-tagged Mus musculus topoisomerase (DNA) II beta (Top2b) Y814F
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The Y814F mutation was introduced by PCR-mediated mutagenesis. The PCR primer contains the A to T point mutation at the nucleotide position 2441 of the mouse Top2b cDNA.
Addgene QC sequencing found an A253V mutation, it should not effect the function of the plasmid.
Proper citation: RRID:Addgene_53455 Copy
Species: Mus musculus
Genetic Insert: mitogen-activated protein kinase 8 interacting protein 3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcdna3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53458 Copy
Species: Mus musculus
Genetic Insert: Rab1
Vector Backbone Description: Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53518 Copy
Species: Mus musculus
Genetic Insert: Rab35
Vector Backbone Description: Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53516 Copy
Species: Mus musculus
Genetic Insert: Rab35
Vector Backbone Description: Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53512 Copy
Species: Mus musculus
Genetic Insert: Rab35
Vector Backbone Description: Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53513 Copy
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